Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels
The temperature- and pH-responsive poly(<i>N</i>-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone whic...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/3/426 |
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| author | Ivana Gajić Snežana Ilić-Stojanović Ana Dinić Aleksandar Zdravković Ljiljana Stanojević Vesna Nikolić Ljubiša Nikolić |
| author_facet | Ivana Gajić Snežana Ilić-Stojanović Ana Dinić Aleksandar Zdravković Ljiljana Stanojević Vesna Nikolić Ljubiša Nikolić |
| author_sort | Ivana Gajić |
| collection | DOAJ |
| description | The temperature- and pH-responsive poly(<i>N</i>-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to its poor aqueous solubility and extensive first-pass metabolism, biochanin A has low bioavailability. The aim of this work was to incorporate biochanin A into the synthesized p(NIPAM-co-AA) copolymer and to examine its release at the body temperature and pH values that correspond to pH values of vaginal and rectal cavities. The amount of released biochanin A was monitored by the ultra-visible spectroscopy (UV-Vis) method. The structure of synthesized p(NIPAM-co-AA) copolymer and copolymer with incorporated biochanin A were characterized by using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The content of residual monomers in the synthesized copolymer was analyzed by using the high-pressure liquid chromatography (HPLC) method. The swelling behavior of p(NIPAM-co-AA) copolymer was monitored in relation to the temperature and pH values of the surrounding medium. For modelling the process of p(NIPAM-co-AA) copolymer swelling, the full three-level factorial design was applied. |
| first_indexed | 2024-03-09T03:20:26Z |
| format | Article |
| id | doaj.art-84387947546b4c329b9990f90f671ee0 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T03:20:26Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-84387947546b4c329b9990f90f671ee02023-12-03T15:10:18ZengMDPI AGPolymers2073-43602021-01-0113342610.3390/polym13030426Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer HydrogelsIvana Gajić0Snežana Ilić-Stojanović1Ana Dinić2Aleksandar Zdravković3Ljiljana Stanojević4Vesna Nikolić5Ljubiša Nikolić6Faculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaFaculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaFaculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaDepartment of Technology and Art Studies, Academy of Professional Studies South Serbia, Leskovac, Vilema Pušmana 17, 16000 Leskovac, SerbiaFaculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaFaculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaFaculty of Technology, University of Niš, Bulevar Oslobodjenja 124, 16000 Leskovac, SerbiaThe temperature- and pH-responsive poly(<i>N</i>-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to its poor aqueous solubility and extensive first-pass metabolism, biochanin A has low bioavailability. The aim of this work was to incorporate biochanin A into the synthesized p(NIPAM-co-AA) copolymer and to examine its release at the body temperature and pH values that correspond to pH values of vaginal and rectal cavities. The amount of released biochanin A was monitored by the ultra-visible spectroscopy (UV-Vis) method. The structure of synthesized p(NIPAM-co-AA) copolymer and copolymer with incorporated biochanin A were characterized by using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The content of residual monomers in the synthesized copolymer was analyzed by using the high-pressure liquid chromatography (HPLC) method. The swelling behavior of p(NIPAM-co-AA) copolymer was monitored in relation to the temperature and pH values of the surrounding medium. For modelling the process of p(NIPAM-co-AA) copolymer swelling, the full three-level factorial design was applied.https://www.mdpi.com/2073-4360/13/3/426poly(<i>N</i>-isopropylacrylamide-co-acrylic acid)hydrogelswelling kineticslyophilizationresidual reactantsbiochanin A |
| spellingShingle | Ivana Gajić Snežana Ilić-Stojanović Ana Dinić Aleksandar Zdravković Ljiljana Stanojević Vesna Nikolić Ljubiša Nikolić Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels Polymers poly(<i>N</i>-isopropylacrylamide-co-acrylic acid) hydrogel swelling kinetics lyophilization residual reactants biochanin A |
| title | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
| title_full | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
| title_fullStr | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
| title_full_unstemmed | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
| title_short | Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels |
| title_sort | modified biochanin a release from dual ph and thermo responsive copolymer hydrogels |
| topic | poly(<i>N</i>-isopropylacrylamide-co-acrylic acid) hydrogel swelling kinetics lyophilization residual reactants biochanin A |
| url | https://www.mdpi.com/2073-4360/13/3/426 |
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