Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants
Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01161/full |
| _version_ | 1818303587128705024 |
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| author | Aimee C. McKinnon Travis R. Glare Hayley J. Ridgway Artemio Mendoza-Mendoza Andrew Holyoake William K. Godsoe Jennifer L. Bufford |
| author_facet | Aimee C. McKinnon Travis R. Glare Hayley J. Ridgway Artemio Mendoza-Mendoza Andrew Holyoake William K. Godsoe Jennifer L. Bufford |
| author_sort | Aimee C. McKinnon |
| collection | DOAJ |
| description | Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroRespTM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization. |
| first_indexed | 2024-12-13T05:57:10Z |
| format | Article |
| id | doaj.art-84392a0cf20c4f57afb02070833f36ab |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T05:57:10Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-84392a0cf20c4f57afb02070833f36ab2022-12-21T23:57:24ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01161335261Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays PlantsAimee C. McKinnon0Travis R. Glare1Hayley J. Ridgway2Artemio Mendoza-Mendoza3Andrew Holyoake4William K. Godsoe5Jennifer L. Bufford6Bio-Protection Research Centre, Lincoln University, Christchurch, New ZealandBio-Protection Research Centre, Lincoln University, Christchurch, New ZealandThe New Zealand Institute for Plant & Food Research Limited, Christchurch, New ZealandBio-Protection Research Centre, Lincoln University, Christchurch, New ZealandBio-Protection Research Centre, Lincoln University, Christchurch, New ZealandBio-Protection Research Centre, Lincoln University, Christchurch, New ZealandBio-Protection Research Centre, Lincoln University, Christchurch, New ZealandEntomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroRespTM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.https://www.frontiersin.org/article/10.3389/fmicb.2018.01161/fullentomopathogenic fungiendophytesplant interactionagroecosystemsbiocontrolmultitrophic interactions |
| spellingShingle | Aimee C. McKinnon Travis R. Glare Hayley J. Ridgway Artemio Mendoza-Mendoza Andrew Holyoake William K. Godsoe Jennifer L. Bufford Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants Frontiers in Microbiology entomopathogenic fungi endophytes plant interaction agroecosystems biocontrol multitrophic interactions |
| title | Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants |
| title_full | Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants |
| title_fullStr | Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants |
| title_full_unstemmed | Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants |
| title_short | Detection of the Entomopathogenic Fungus Beauveria bassiana in the Rhizosphere of Wound-Stressed Zea mays Plants |
| title_sort | detection of the entomopathogenic fungus beauveria bassiana in the rhizosphere of wound stressed zea mays plants |
| topic | entomopathogenic fungi endophytes plant interaction agroecosystems biocontrol multitrophic interactions |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01161/full |
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