Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar
<p>Abstract</p> <p>Background</p> <p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (<it>Populus trichocarpa </it>× <it>P. deltoides) </it>using proteomics methods, and their sieve element localiz...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-08-01
|
| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/191 |
| _version_ | 1819086531853287424 |
|---|---|
| author | Dafoe Nicole J Gowen Brent E Constabel C Peter |
| author_facet | Dafoe Nicole J Gowen Brent E Constabel C Peter |
| author_sort | Dafoe Nicole J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (<it>Populus trichocarpa </it>× <it>P. deltoides) </it>using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</p> <p>Results</p> <p>Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</p> <p>Conclusions</p> <p>TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.</p> |
| first_indexed | 2024-12-21T21:21:44Z |
| format | Article |
| id | doaj.art-843a3bde39114af7a92024bfb351b589 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T21:21:44Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-843a3bde39114af7a92024bfb351b5892022-12-21T18:49:53ZengBMCBMC Plant Biology1471-22292010-08-0110119110.1186/1471-2229-10-191Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplarDafoe Nicole JGowen Brent EConstabel C Peter<p>Abstract</p> <p>Background</p> <p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (<it>Populus trichocarpa </it>× <it>P. deltoides) </it>using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</p> <p>Results</p> <p>Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</p> <p>Conclusions</p> <p>TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.</p>http://www.biomedcentral.com/1471-2229/10/191 |
| spellingShingle | Dafoe Nicole J Gowen Brent E Constabel C Peter Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar BMC Plant Biology |
| title | Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| title_full | Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| title_fullStr | Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| title_full_unstemmed | Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| title_short | Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| title_sort | thaumatin like proteins are differentially expressed and localized in phloem tissues of hybrid poplar |
| url | http://www.biomedcentral.com/1471-2229/10/191 |
| work_keys_str_mv | AT dafoenicolej thaumatinlikeproteinsaredifferentiallyexpressedandlocalizedinphloemtissuesofhybridpoplar AT gowenbrente thaumatinlikeproteinsaredifferentiallyexpressedandlocalizedinphloemtissuesofhybridpoplar AT constabelcpeter thaumatinlikeproteinsaredifferentiallyexpressedandlocalizedinphloemtissuesofhybridpoplar |