Light-Up Split Broccoli Aptamer as a Versatile Tool for RNA Assembly Monitoring in Cell-Free TX-TL Systems, Hybrid RNA/DNA Origami Tagging and DNA Biosensing

Light-Up Split Broccoli Aptamer as a Versatile Tool for RNA Assembly Monitoring in Cell-Free TX-TL Systems, Hybrid RNA/DNA Origami Tagging and DNA Biosensing

Binary light-up aptamers are intriguing and emerging tools with potential in different fields. Herein, we demonstrate the versatility of a split Broccoli aptamer system able to turn on the fluorescence signal only in the presence of a complementary sequence. First, an RNA three-way junction harbouri...

Full description

Bibliographic Details
Main Authors: Emanuela Torelli, Ben Shirt-Ediss, Silvia A. Navarro, Marisa Manzano, Priya Vizzini, Natalio Krasnogor
Format: Article
Language:English
Published: MDPI AG 2023-05-01
Series:International Journal of Molecular Sciences
Subjects:
light-up split Broccoli aptamer
RNA self-assembly monitoring
cell-free TX-TL system
hybrid RNA/DNA origami
target DNA detection
<i>Campylobacter</i> spp.
Online Access:https://www.mdpi.com/1422-0067/24/10/8483

Internet

https://www.mdpi.com/1422-0067/24/10/8483

Similar Items