Conformation of G-quadruplex Controlled by Click Reaction
G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformatio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4339 |
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| author | Chao-Da Xiao Zhi-Yong He Chuan-Xin Guo Xiang-Chun Shen Yan Xu |
| author_facet | Chao-Da Xiao Zhi-Yong He Chuan-Xin Guo Xiang-Chun Shen Yan Xu |
| author_sort | Chao-Da Xiao |
| collection | DOAJ |
| description | G-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2′-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence. |
| first_indexed | 2024-03-10T16:08:47Z |
| format | Article |
| id | doaj.art-843c47c6b80e43bb97b105d655f177a6 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:08:47Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-843c47c6b80e43bb97b105d655f177a62023-11-20T14:37:35ZengMDPI AGMolecules1420-30492020-09-012518433910.3390/molecules25184339Conformation of G-quadruplex Controlled by Click ReactionChao-Da Xiao0Zhi-Yong He1Chuan-Xin Guo2Xiang-Chun Shen3Yan Xu4State Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guiyang 550025, ChinaMedical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, JapanNucleic Acid Division, Shanghai Cell Therapy Group Co. Ltd., Jiading, Shanghai 201805, ChinaState Key Laboratory of Functions and Applications of Medicinal Plants, School of Pharmaceutical Sciences, Guizhou Medical University, University Town, Guian New District, Guiyang 550025, ChinaMedical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, JapanG-quadruplexes are non-canonical four stranded secondary structures possessing great biological importance. Controlling G-quadruplex conformation for further regulating biological processes is both exciting and challenging. In this study, we described a method for regulating G-quadruplex conformation by click chemistry for the first time. 8-ethynyl-2′-deoxyguanosine was synthesized and incorporated into a 12-nt telomere DNA sequence. Such a sequence, at first, formed mixed parallel/anti-parallel G-quadruplexes, while it changed to anti-parallel after reaction with azidobenzene. Meanwhile, the click reaction can give the sequence intense fluorescence.https://www.mdpi.com/1420-3049/25/18/4339G-quadruplexconformation changeclick reactionNMRcircular dichroism |
| spellingShingle | Chao-Da Xiao Zhi-Yong He Chuan-Xin Guo Xiang-Chun Shen Yan Xu Conformation of G-quadruplex Controlled by Click Reaction Molecules G-quadruplex conformation change click reaction NMR circular dichroism |
| title | Conformation of G-quadruplex Controlled by Click Reaction |
| title_full | Conformation of G-quadruplex Controlled by Click Reaction |
| title_fullStr | Conformation of G-quadruplex Controlled by Click Reaction |
| title_full_unstemmed | Conformation of G-quadruplex Controlled by Click Reaction |
| title_short | Conformation of G-quadruplex Controlled by Click Reaction |
| title_sort | conformation of g quadruplex controlled by click reaction |
| topic | G-quadruplex conformation change click reaction NMR circular dichroism |
| url | https://www.mdpi.com/1420-3049/25/18/4339 |
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