Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation
Abstract Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational mod...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-41482-5 |
| _version_ | 1797558458054082560 |
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| author | Ran Wang Xianfa Yang Jiehui Chen Lin Zhang Jonathan A. Griffiths Guizhong Cui Yingying Chen Yun Qian Guangdun Peng Jinsong Li Liantang Wang John C. Marioni Patrick P. L. Tam Naihe Jing |
| author_facet | Ran Wang Xianfa Yang Jiehui Chen Lin Zhang Jonathan A. Griffiths Guizhong Cui Yingying Chen Yun Qian Guangdun Peng Jinsong Li Liantang Wang John C. Marioni Patrick P. L. Tam Naihe Jing |
| author_sort | Ran Wang |
| collection | DOAJ |
| description | Abstract Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we constructed at single-cell resolution, a spatio-temporal transcriptome of cell populations in the germ-layers of gastrula-stage mouse embryos. This molecular atlas enables the inference of molecular network activity underpinning the specification and differentiation of the germ-layer tissue lineages. Heterogeneity analysis of cellular composition at defined positions in the epiblast revealed progressive diversification of cell types. The single-cell transcriptome revealed an enhanced BMP signaling activity in the right-side mesoderm of late-gastrulation embryo. Perturbation of asymmetric BMP signaling activity at late gastrulation led to randomization of left-right molecular asymmetry in the lateral mesoderm of early-somite-stage embryo. These findings indicate the asymmetric BMP activity during gastrulation may be critical for the symmetry breaking process. |
| first_indexed | 2024-03-10T17:31:42Z |
| format | Article |
| id | doaj.art-843d895fd4d14dc49ffa9a2deb1ffa4e |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:31:42Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-843d895fd4d14dc49ffa9a2deb1ffa4e2023-11-20T10:00:29ZengNature PortfolioNature Communications2041-17232023-09-0114111810.1038/s41467-023-41482-5Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulationRan Wang0Xianfa Yang1Jiehui Chen2Lin Zhang3Jonathan A. Griffiths4Guizhong Cui5Yingying Chen6Yun Qian7Guangdun Peng8Jinsong Li9Liantang Wang10John C. Marioni11Patrick P. L. Tam12Naihe Jing13State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesGuangzhou National LaboratoryState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesCancer Research UK Cambridge Institute, University of CambridgeGuangzhou National LaboratoryGuangzhou National LaboratoryState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesSchool of Mathematics, Northwest UniversityCancer Research UK Cambridge Institute, University of CambridgeEmbryology Research Unit, Children’s Medical Research Institute, University of SydneyState Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of SciencesAbstract Understanding of the molecular drivers of lineage diversification and tissue patterning during primary germ layer development requires in-depth knowledge of the dynamic molecular trajectories of cell lineages across a series of developmental stages of gastrulation. Through computational modeling, we constructed at single-cell resolution, a spatio-temporal transcriptome of cell populations in the germ-layers of gastrula-stage mouse embryos. This molecular atlas enables the inference of molecular network activity underpinning the specification and differentiation of the germ-layer tissue lineages. Heterogeneity analysis of cellular composition at defined positions in the epiblast revealed progressive diversification of cell types. The single-cell transcriptome revealed an enhanced BMP signaling activity in the right-side mesoderm of late-gastrulation embryo. Perturbation of asymmetric BMP signaling activity at late gastrulation led to randomization of left-right molecular asymmetry in the lateral mesoderm of early-somite-stage embryo. These findings indicate the asymmetric BMP activity during gastrulation may be critical for the symmetry breaking process.https://doi.org/10.1038/s41467-023-41482-5 |
| spellingShingle | Ran Wang Xianfa Yang Jiehui Chen Lin Zhang Jonathan A. Griffiths Guizhong Cui Yingying Chen Yun Qian Guangdun Peng Jinsong Li Liantang Wang John C. Marioni Patrick P. L. Tam Naihe Jing Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation Nature Communications |
| title | Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| title_full | Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| title_fullStr | Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| title_full_unstemmed | Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| title_short | Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| title_sort | time space and single cell resolved tissue lineage trajectories and laterality of body plan at gastrulation |
| url | https://doi.org/10.1038/s41467-023-41482-5 |
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