Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair
The self-healing potential of each tissue belongs to endogenous stem cells residing in the tissue; however, there are currently no reports mentioned for the isolation of human rotator cuff-derived mesenchymal stem cells (RC-MSCs) since. To isolate RC-MSCs, minced rotator cuff samples were first dige...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2013-03-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368912X656090 |
| _version_ | 1818149116914434048 |
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| author | Chih-Chien Tsai Tung-Fu Huang Hsiao-Li Ma En-Rung Chiang Shih-Chieh Hung M.D., Ph.D. |
| author_facet | Chih-Chien Tsai Tung-Fu Huang Hsiao-Li Ma En-Rung Chiang Shih-Chieh Hung M.D., Ph.D. |
| author_sort | Chih-Chien Tsai |
| collection | DOAJ |
| description | The self-healing potential of each tissue belongs to endogenous stem cells residing in the tissue; however, there are currently no reports mentioned for the isolation of human rotator cuff-derived mesenchymal stem cells (RC-MSCs) since. To isolate RC-MSCs, minced rotator cuff samples were first digested with enzymes and the single cell suspensions were seeded in plastic culture dishes. Twenty-four hours later, nonadherent cells were removed and the adherent cells were further cultured. The RC-MSCs had fibroblast-like morphology and were positive for the putative surface markers of MSCs, such as CD44, CD73, CD90, CD105, and CD166, and negative for the putative markers of hematopoietic cells, such as CD34, CD45, and CD133. Similar to BM-MSCs, RC-MSCs were demonstrated to have the potential to undergo osteogenic, adipogenic, and chondrogenic differentiation. Upon induction in the defined media, RC-MSCs also expressed lineage-specific genes, such as Runx 2 and osteocalcin in osteogenic induction, PPAR-γ and LPL in adipogenic differentiation, and aggrecan and Col2a1 in chondrogenic differentiation. The multipotent feature of RC-MSCs in the myogenic injury model was further strengthened by the increase in myogenic potential both in vitro and in vivo when compared with BM-MSCs. These results demonstrate the successful isolation of MSCs from human rotator cuffs and encourage the application of RC-MSCs in myogenic regeneration. |
| first_indexed | 2024-12-11T13:01:56Z |
| format | Article |
| id | doaj.art-843f614f7a6e4bc48d43a46ae1d61877 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-11T13:01:56Z |
| publishDate | 2013-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-843f614f7a6e4bc48d43a46ae1d618772022-12-22T01:06:27ZengSAGE PublishingCell Transplantation0963-68971555-38922013-03-012210.3727/096368912X656090Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon RepairChih-Chien Tsai0Tung-Fu Huang1Hsiao-Li Ma2En-Rung Chiang3Shih-Chieh Hung M.D., Ph.D.4Stem Cell Laboratory, Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei, TaiwanOrthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, TaiwanOrthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, TaiwanOrthopaedics and Traumatology, Taipei Veterans General Hospital, Taipei, TaiwanInstitute of Pharmacology, National Yang-Ming University, Taipei, TaiwanThe self-healing potential of each tissue belongs to endogenous stem cells residing in the tissue; however, there are currently no reports mentioned for the isolation of human rotator cuff-derived mesenchymal stem cells (RC-MSCs) since. To isolate RC-MSCs, minced rotator cuff samples were first digested with enzymes and the single cell suspensions were seeded in plastic culture dishes. Twenty-four hours later, nonadherent cells were removed and the adherent cells were further cultured. The RC-MSCs had fibroblast-like morphology and were positive for the putative surface markers of MSCs, such as CD44, CD73, CD90, CD105, and CD166, and negative for the putative markers of hematopoietic cells, such as CD34, CD45, and CD133. Similar to BM-MSCs, RC-MSCs were demonstrated to have the potential to undergo osteogenic, adipogenic, and chondrogenic differentiation. Upon induction in the defined media, RC-MSCs also expressed lineage-specific genes, such as Runx 2 and osteocalcin in osteogenic induction, PPAR-γ and LPL in adipogenic differentiation, and aggrecan and Col2a1 in chondrogenic differentiation. The multipotent feature of RC-MSCs in the myogenic injury model was further strengthened by the increase in myogenic potential both in vitro and in vivo when compared with BM-MSCs. These results demonstrate the successful isolation of MSCs from human rotator cuffs and encourage the application of RC-MSCs in myogenic regeneration.https://doi.org/10.3727/096368912X656090 |
| spellingShingle | Chih-Chien Tsai Tung-Fu Huang Hsiao-Li Ma En-Rung Chiang Shih-Chieh Hung M.D., Ph.D. Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair Cell Transplantation |
| title | Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair |
| title_full | Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair |
| title_fullStr | Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair |
| title_full_unstemmed | Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair |
| title_short | Isolation of Mesenchymal Stem Cells from Shoulder Rotator Cuff: A Potential Source for Muscle and Tendon Repair |
| title_sort | isolation of mesenchymal stem cells from shoulder rotator cuff a potential source for muscle and tendon repair |
| url | https://doi.org/10.3727/096368912X656090 |
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