Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles
This study demonstrates a novel colorimetric method for clenbuterol (CLB) detection based on the peroxidase-like activity of SiO2-coated Fe3O4 nanoparticles (Fe3O4@mSiO2) modified with montmorillonite (MMT) and sodium dodecyl sulfate (SDS), namely, Fe3O4@mSiO2@MMT-SDS. Fe3O4@mSiO2@MMT-SDS nanopartic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223008134 |
| _version_ | 1797615933797171200 |
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| author | Li Wu Qijie Mo Shoulian Wei Ruiping Gao Shuai Zhang Xiaohua Zhu |
| author_facet | Li Wu Qijie Mo Shoulian Wei Ruiping Gao Shuai Zhang Xiaohua Zhu |
| author_sort | Li Wu |
| collection | DOAJ |
| description | This study demonstrates a novel colorimetric method for clenbuterol (CLB) detection based on the peroxidase-like activity of SiO2-coated Fe3O4 nanoparticles (Fe3O4@mSiO2) modified with montmorillonite (MMT) and sodium dodecyl sulfate (SDS), namely, Fe3O4@mSiO2@MMT-SDS. Fe3O4@mSiO2@MMT-SDS nanoparticles were prepared by modifying Fe3O4@mSiO2 with MMT and SDS to impart negative charges to the particle surfaces. The structural properties, steady-state kinetics, catalytic performance, and reaction conditions of the as-prepared nanoparticles were analyzed. Fe3O4@mSiO2@MMT-SDS nanoparticles exhibited excellent dispersion and enzyme-like activity, which improved the affinity with substrate (TMB and H2O2) for the catalytic oxidation of CLB by H2O2, turning the solution blue in the presence of the 3,3ʹ, 5,5ʹ-tetramethylbenzidine chromogen. Under optimal reaction conditions, a strong linear correlation between the absorbance of solution and CLB concentration (R2 = 0.9983) was observed. Here, the limit of detection, spike recovery, and relative standard deviation were 9.6 × 10-9 mol·L-1, 94.2 %–102.2 %, and 2.2 %–4.4 %, respectively. The proposed method is a simple, fast, cost-efficient, and sensitive assay, which can be used to rapidly and accurately screen CLB in pork and animal feeds. |
| first_indexed | 2024-03-11T07:34:02Z |
| format | Article |
| id | doaj.art-8440cfefaf4443fb93c4d382ea4b82f3 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-11T07:34:02Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-8440cfefaf4443fb93c4d382ea4b82f32023-11-17T05:26:07ZengElsevierArabian Journal of Chemistry1878-53522023-12-011612105351Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticlesLi Wu0Qijie Mo1Shoulian Wei2Ruiping Gao3Shuai Zhang4Xiaohua Zhu5School of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, ChinaSchool of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, ChinaSchool of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing 526061, ChinaSchool of Environment and Resources, Chongqing Technology and Business University, Chongqing 400067, ChinaSchool of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing 526061, China; Corresponding author.Key Laboratory Marine Biological Waste and Comprehensive Utilization of Guangdong Province, Zhanjiang 524051, ChinaThis study demonstrates a novel colorimetric method for clenbuterol (CLB) detection based on the peroxidase-like activity of SiO2-coated Fe3O4 nanoparticles (Fe3O4@mSiO2) modified with montmorillonite (MMT) and sodium dodecyl sulfate (SDS), namely, Fe3O4@mSiO2@MMT-SDS. Fe3O4@mSiO2@MMT-SDS nanoparticles were prepared by modifying Fe3O4@mSiO2 with MMT and SDS to impart negative charges to the particle surfaces. The structural properties, steady-state kinetics, catalytic performance, and reaction conditions of the as-prepared nanoparticles were analyzed. Fe3O4@mSiO2@MMT-SDS nanoparticles exhibited excellent dispersion and enzyme-like activity, which improved the affinity with substrate (TMB and H2O2) for the catalytic oxidation of CLB by H2O2, turning the solution blue in the presence of the 3,3ʹ, 5,5ʹ-tetramethylbenzidine chromogen. Under optimal reaction conditions, a strong linear correlation between the absorbance of solution and CLB concentration (R2 = 0.9983) was observed. Here, the limit of detection, spike recovery, and relative standard deviation were 9.6 × 10-9 mol·L-1, 94.2 %–102.2 %, and 2.2 %–4.4 %, respectively. The proposed method is a simple, fast, cost-efficient, and sensitive assay, which can be used to rapidly and accurately screen CLB in pork and animal feeds.http://www.sciencedirect.com/science/article/pii/S1878535223008134Magnetic nanoparticleEnzyme-likeColorimetryClenbuterol |
| spellingShingle | Li Wu Qijie Mo Shoulian Wei Ruiping Gao Shuai Zhang Xiaohua Zhu Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles Arabian Journal of Chemistry Magnetic nanoparticle Enzyme-like Colorimetry Clenbuterol |
| title | Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles |
| title_full | Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles |
| title_fullStr | Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles |
| title_full_unstemmed | Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles |
| title_short | Colorimetric method for clenbuterol detection based on the enzyme-like activity of magnetic Fe3O4@mSiO2@MMT-SDS nanoparticles |
| title_sort | colorimetric method for clenbuterol detection based on the enzyme like activity of magnetic fe3o4 msio2 mmt sds nanoparticles |
| topic | Magnetic nanoparticle Enzyme-like Colorimetry Clenbuterol |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223008134 |
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