Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity
Mona Al-Ahmad,1,2,* Asmaa Ali,2– 4,* Mohammad Z Haider5 1Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait; 2Department of Allergy, Al-Rashed Allergy Center, Ministry of Health, Kuwait City, Kuwait; 3Department of Laboratory Medicine, School of M...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2023-11-01
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| Series: | Journal of Asthma and Allergy |
| Subjects: | |
| Online Access: | https://www.dovepress.com/interleukin-4-c590t-gene-polymorphism-in-association-with-asthma-sever-peer-reviewed-fulltext-article-JAA |
| _version_ | 1797536459545116672 |
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| author | Al-Ahmad M Ali A Haider MZ |
| author_facet | Al-Ahmad M Ali A Haider MZ |
| author_sort | Al-Ahmad M |
| collection | DOAJ |
| description | Mona Al-Ahmad,1,2,* Asmaa Ali,2– 4,* Mohammad Z Haider5 1Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait; 2Department of Allergy, Al-Rashed Allergy Center, Ministry of Health, Kuwait City, Kuwait; 3Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, People’s Republic of China; 4Department of Pulmonary Medicine, Abbassia Chest Hospital, Ministry of Health, Cairo, Egypt; 5Department of Pediatrics, College of Medicine, Kuwait University, Kuwait City, Kuwait*These authors contributed equally to this workCorrespondence: Mona Al-Ahmad, Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait, Tel +965-24636515, Fax +965-25332719, Email mona.alahmad@ku.edu.kwBackground: A significant link between T allele of the IL-4 (C590T) gene and developing asthma in some populations was reported. However, no study discussed the link between IL-4 (C590T) gene polymorphism and asthma severity groups (mild and severe). This study investigated the link between IL-4 gene variation and asthma severity.Methods: The study included 215 asthmatic patients, of which 102 had mild asthma, and 126 participants were healthy controls. A previously published polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to identify various IL-4 (C590T) gene polymorphism genotypes.Results: The T allele frequency was higher in mild asthma (p=0.002) but not in severe asthma (p=0.12) compared to controls. In mild asthma, the CT genotype and (CT+TT versus CC) increased the likelihood of asthma threefold (p< 0.001, 0.001). However, no significant association with severe asthma was found in either genetic model. Stratification analysis showed that the C allele and CC genotype increased the risk of severe asthma (p=0.01). The recessive genetic model indicated a decrease in the risk of severe asthma (OR=0.5, p=0.01) in the non-adjusted regression analysis. Adjusting for age, sex, and other risk factors revealed that the IL-4 gene polymorphism did not influence the risk of severe asthma (OR=0.92, p=0.80); however, being an elderly female with a history of childhood-onset disease and associated nasal polyp (NP) increased the likelihood of severe asthma, OR=1.08, 2.01, 2.36, 8.42; p< 0.001, 0.05, 0.05, < 0.001, respectively.Conclusion: The T allele and CT genotype in the co-dominant genetic model and the (CT+TT) genotype in the recessive model were found to have a higher likelihood of developing mild asthma but not severe asthma; severe asthma was found to be higher in elderly females with a history of childhood-onset disease and associated nasal polyps.Keywords: IL-4 (C590T) gene polymorphism, mild and severe asthma, nasal polyp |
| first_indexed | 2024-03-10T11:59:59Z |
| format | Article |
| id | doaj.art-8445c554c3f54a20861a69712c8b73ca |
| institution | Directory Open Access Journal |
| issn | 1178-6965 |
| language | English |
| last_indexed | 2024-03-10T11:59:59Z |
| publishDate | 2023-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Asthma and Allergy |
| spelling | doaj.art-8445c554c3f54a20861a69712c8b73ca2023-11-21T17:01:05ZengDove Medical PressJournal of Asthma and Allergy1178-69652023-11-01Volume 161269127888362Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma SeverityAl-Ahmad MAli AHaider MZMona Al-Ahmad,1,2,* Asmaa Ali,2– 4,* Mohammad Z Haider5 1Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait; 2Department of Allergy, Al-Rashed Allergy Center, Ministry of Health, Kuwait City, Kuwait; 3Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, People’s Republic of China; 4Department of Pulmonary Medicine, Abbassia Chest Hospital, Ministry of Health, Cairo, Egypt; 5Department of Pediatrics, College of Medicine, Kuwait University, Kuwait City, Kuwait*These authors contributed equally to this workCorrespondence: Mona Al-Ahmad, Department of Microbiology, College of Medicine, Kuwait University, Kuwait City, Kuwait, Tel +965-24636515, Fax +965-25332719, Email mona.alahmad@ku.edu.kwBackground: A significant link between T allele of the IL-4 (C590T) gene and developing asthma in some populations was reported. However, no study discussed the link between IL-4 (C590T) gene polymorphism and asthma severity groups (mild and severe). This study investigated the link between IL-4 gene variation and asthma severity.Methods: The study included 215 asthmatic patients, of which 102 had mild asthma, and 126 participants were healthy controls. A previously published polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to identify various IL-4 (C590T) gene polymorphism genotypes.Results: The T allele frequency was higher in mild asthma (p=0.002) but not in severe asthma (p=0.12) compared to controls. In mild asthma, the CT genotype and (CT+TT versus CC) increased the likelihood of asthma threefold (p< 0.001, 0.001). However, no significant association with severe asthma was found in either genetic model. Stratification analysis showed that the C allele and CC genotype increased the risk of severe asthma (p=0.01). The recessive genetic model indicated a decrease in the risk of severe asthma (OR=0.5, p=0.01) in the non-adjusted regression analysis. Adjusting for age, sex, and other risk factors revealed that the IL-4 gene polymorphism did not influence the risk of severe asthma (OR=0.92, p=0.80); however, being an elderly female with a history of childhood-onset disease and associated nasal polyp (NP) increased the likelihood of severe asthma, OR=1.08, 2.01, 2.36, 8.42; p< 0.001, 0.05, 0.05, < 0.001, respectively.Conclusion: The T allele and CT genotype in the co-dominant genetic model and the (CT+TT) genotype in the recessive model were found to have a higher likelihood of developing mild asthma but not severe asthma; severe asthma was found to be higher in elderly females with a history of childhood-onset disease and associated nasal polyps.Keywords: IL-4 (C590T) gene polymorphism, mild and severe asthma, nasal polyphttps://www.dovepress.com/interleukin-4-c590t-gene-polymorphism-in-association-with-asthma-sever-peer-reviewed-fulltext-article-JAAil-4 (c590t) gene polymorphismmild and severe asthmanasal polyp |
| spellingShingle | Al-Ahmad M Ali A Haider MZ Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity Journal of Asthma and Allergy il-4 (c590t) gene polymorphism mild and severe asthma nasal polyp |
| title | Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity |
| title_full | Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity |
| title_fullStr | Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity |
| title_full_unstemmed | Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity |
| title_short | Interleukin-4 (C590T) Gene Polymorphism in Association with Asthma Severity |
| title_sort | interleukin 4 c590t gene polymorphism in association with asthma severity |
| topic | il-4 (c590t) gene polymorphism mild and severe asthma nasal polyp |
| url | https://www.dovepress.com/interleukin-4-c590t-gene-polymorphism-in-association-with-asthma-sever-peer-reviewed-fulltext-article-JAA |
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