Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India
The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and A...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/9/1449 |
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| author | Tushar Kumar Dey Johanna Frida Lindahl Åke Lundkvist Delia Grace Ram Pratim Deka Rajeswari Shome Samiran Bandyopadhyay Naresh Kumar Goyal Garima Sharma Bibek Ranjan Shome |
| author_facet | Tushar Kumar Dey Johanna Frida Lindahl Åke Lundkvist Delia Grace Ram Pratim Deka Rajeswari Shome Samiran Bandyopadhyay Naresh Kumar Goyal Garima Sharma Bibek Ranjan Shome |
| author_sort | Tushar Kumar Dey |
| collection | DOAJ |
| description | The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla<sub>CMY</sub>, bla<sub>MOX</sub>, bla<sub>FOX</sub>, bla<sub>EBC</sub>, and bla<sub>DHA</sub>, associated with AmpC production. Additionally, bla<sub>CTX-M1</sub>, bla<sub>SHV</sub>, and bla<sub>TEM</sub> were detected as ESBL producers, while bla<sub>VIM</sub>, bla<sub>IMP</sub>, bla<sub>SPM</sub>, bla<sub>SIM</sub>, and bla<sub>GIM</sub> were identified as MBL producers. Notably, <i>Shigella</i> spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance. |
| first_indexed | 2024-03-10T23:06:35Z |
| format | Article |
| id | doaj.art-84463de094ee427e8b67c8a66898336d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T23:06:35Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-84463de094ee427e8b67c8a66898336d2023-11-19T09:17:55ZengMDPI AGAntibiotics2079-63822023-09-01129144910.3390/antibiotics12091449Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in IndiaTushar Kumar Dey0Johanna Frida Lindahl1Åke Lundkvist2Delia Grace3Ram Pratim Deka4Rajeswari Shome5Samiran Bandyopadhyay6Naresh Kumar Goyal7Garima Sharma8Bibek Ranjan Shome9Department of Biosciences, International Livestock Research Institute, Nairobi 00100, KenyaDepartment of Biosciences, International Livestock Research Institute, Nairobi 00100, KenyaZoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, SwedenDepartment of Biosciences, International Livestock Research Institute, Nairobi 00100, KenyaInternational Livestock Research Institute, Regional Office for South Asia, New Delhi 110012, IndiaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaEastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata 700037, IndiaDairy Microbiology Division, National Dairy Research Institute, Karnal 132001, IndiaDepartment of Biosciences, International Livestock Research Institute, Nairobi 00100, KenyaICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, IndiaThe consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing β-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of β-lactamase genes was 10% (confidence interval (CI) (7–13)), with higher rates in Haryana (13%, CI (9–19)) compared to Assam (7%, CI (4–11)). The identified β-lactamase genes in isolates were bla<sub>CMY</sub>, bla<sub>MOX</sub>, bla<sub>FOX</sub>, bla<sub>EBC</sub>, and bla<sub>DHA</sub>, associated with AmpC production. Additionally, bla<sub>CTX-M1</sub>, bla<sub>SHV</sub>, and bla<sub>TEM</sub> were detected as ESBL producers, while bla<sub>VIM</sub>, bla<sub>IMP</sub>, bla<sub>SPM</sub>, bla<sub>SIM</sub>, and bla<sub>GIM</sub> were identified as MBL producers. Notably, <i>Shigella</i> spp. were the dominant β-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent β-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of β-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of β-lactam resistance.https://www.mdpi.com/2079-6382/12/9/1449antimicrobial resistanceAMRenterobacteriaceaeβ-lactamaseESBLMBL |
| spellingShingle | Tushar Kumar Dey Johanna Frida Lindahl Åke Lundkvist Delia Grace Ram Pratim Deka Rajeswari Shome Samiran Bandyopadhyay Naresh Kumar Goyal Garima Sharma Bibek Ranjan Shome Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India Antibiotics antimicrobial resistance AMR enterobacteriaceae β-lactamase ESBL MBL |
| title | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
| title_full | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
| title_fullStr | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
| title_full_unstemmed | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
| title_short | Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India |
| title_sort | analyses of extended spectrum β lactamase metallo β lactamase and ampc β lactamase producing enterobacteriaceae from the dairy value chain in india |
| topic | antimicrobial resistance AMR enterobacteriaceae β-lactamase ESBL MBL |
| url | https://www.mdpi.com/2079-6382/12/9/1449 |
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