Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens
Past immunological studies in broilers focused on juveniles within the rapid pre-slaughter growth period and may not reflect adult immune responses, particularly in breeders managed with chronic feed restriction (R). The study aimed to assess innate immune cell functions in respect to R vs. ad libit...
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/6/1742 |
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| author | Pao-Chia Chou Pei-Chi Lin Shu-Wei Wu Chien-Kai Wang Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen |
| author_facet | Pao-Chia Chou Pei-Chi Lin Shu-Wei Wu Chien-Kai Wang Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen |
| author_sort | Pao-Chia Chou |
| collection | DOAJ |
| description | Past immunological studies in broilers focused on juveniles within the rapid pre-slaughter growth period and may not reflect adult immune responses, particularly in breeders managed with chronic feed restriction (R). The study aimed to assess innate immune cell functions in respect to R vs. ad libitum (Ad) feed intake in breeder hens with and without dietary 25-hydroxycholecalciferol (25-OH-D<sub>3</sub>) supplementation. Ad-feed intake consistently suppressed IL-1β secretion, respiratory burst, and cell livability in peripheral heterophils and/or monocytes along the feeding trial from the age of 51 to 68 weeks. Supplemental 25-OH-D<sub>3</sub> repressed IL-1β secretion and respiratory burst of both cells mostly in R-hens, but promoted monocyte phagocytosis, chemotaxis, and bacterial killing activity in Ad-hens in accompany with relieved hyperglycemia, hyperlipidemia, and systemic inflammation. Overnight cultures with leukocytes from R-hens confirmed the differential effects of 25-OH-D<sub>3</sub> to rescue immune functions altered by glucose and/or palmitic acid exposure. Studies with specific inhibitors further manifested the operative mechanisms via glucolipotoxicity in a cell type- and function-dependent manner. The results concluded no predominant changes between R- vs. Ad-feed intake on leukocyte defense against pathogens despite some differential differences, but supplemental 25-OH-D<sub>3</sub> exerts more pronounced effects in Ad-hens. |
| first_indexed | 2024-03-10T10:31:21Z |
| format | Article |
| id | doaj.art-84478e22f52d444f9a9aeb22dd11643f |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:31:21Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-84478e22f52d444f9a9aeb22dd11643f2023-11-21T23:39:22ZengMDPI AGAnimals2076-26152021-06-01116174210.3390/ani11061742Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder HensPao-Chia Chou0Pei-Chi Lin1Shu-Wei Wu2Chien-Kai Wang3Thau-Kiong Chung4Rosemary L. Walzem5Lih-Shiuh Lai6Shuen-Ei Chen7Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 40227, TaiwanDSM Nutritional Products Asia Pacific, Singapore 117440, SingaporeDepartment of Poultry Science, Texas A&M University, College Station, TX 77843, USADepartment of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, TaiwanDepartment of Animal Science, National Chung Hsing University, Taichung 40227, TaiwanPast immunological studies in broilers focused on juveniles within the rapid pre-slaughter growth period and may not reflect adult immune responses, particularly in breeders managed with chronic feed restriction (R). The study aimed to assess innate immune cell functions in respect to R vs. ad libitum (Ad) feed intake in breeder hens with and without dietary 25-hydroxycholecalciferol (25-OH-D<sub>3</sub>) supplementation. Ad-feed intake consistently suppressed IL-1β secretion, respiratory burst, and cell livability in peripheral heterophils and/or monocytes along the feeding trial from the age of 51 to 68 weeks. Supplemental 25-OH-D<sub>3</sub> repressed IL-1β secretion and respiratory burst of both cells mostly in R-hens, but promoted monocyte phagocytosis, chemotaxis, and bacterial killing activity in Ad-hens in accompany with relieved hyperglycemia, hyperlipidemia, and systemic inflammation. Overnight cultures with leukocytes from R-hens confirmed the differential effects of 25-OH-D<sub>3</sub> to rescue immune functions altered by glucose and/or palmitic acid exposure. Studies with specific inhibitors further manifested the operative mechanisms via glucolipotoxicity in a cell type- and function-dependent manner. The results concluded no predominant changes between R- vs. Ad-feed intake on leukocyte defense against pathogens despite some differential differences, but supplemental 25-OH-D<sub>3</sub> exerts more pronounced effects in Ad-hens.https://www.mdpi.com/2076-2615/11/6/1742broiler breeder hens25-hydroxycholecalciferolinnate immunityglucolipotoxicityfeed restriction |
| spellingShingle | Pao-Chia Chou Pei-Chi Lin Shu-Wei Wu Chien-Kai Wang Thau-Kiong Chung Rosemary L. Walzem Lih-Shiuh Lai Shuen-Ei Chen Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens Animals broiler breeder hens 25-hydroxycholecalciferol innate immunity glucolipotoxicity feed restriction |
| title | Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens |
| title_full | Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens |
| title_fullStr | Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens |
| title_full_unstemmed | Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens |
| title_short | Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens |
| title_sort | differential modulation of 25 hydroxycholecalciferol on innate immunity of broiler breeder hens |
| topic | broiler breeder hens 25-hydroxycholecalciferol innate immunity glucolipotoxicity feed restriction |
| url | https://www.mdpi.com/2076-2615/11/6/1742 |
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