ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals
T-2 and HT-2 toxins are secondary metabolites of various species of <i>Fusarium</i>. These molecules can have high potential toxic effects for human and animal health. In this work, ELISA and ultra performance liquid chromatography with fluorescence detection (UPLC/FLD) were implemented...
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MDPI AG
2021-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/4/1688 |
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| author | Paola D’Agnello Valeria Vita Cinzia Franchino Luigi Urbano Antonio Curiale Francesca Debegnach Marco Iammarino Giuliana Marchesani Antonio Eugenio Chiaravalle Rita De Pace |
| author_facet | Paola D’Agnello Valeria Vita Cinzia Franchino Luigi Urbano Antonio Curiale Francesca Debegnach Marco Iammarino Giuliana Marchesani Antonio Eugenio Chiaravalle Rita De Pace |
| author_sort | Paola D’Agnello |
| collection | DOAJ |
| description | T-2 and HT-2 toxins are secondary metabolites of various species of <i>Fusarium</i>. These molecules can have high potential toxic effects for human and animal health. In this work, ELISA and ultra performance liquid chromatography with fluorescence detection (UPLC/FLD) were implemented and validated as screening and confirmatory tests for the detection of these two toxins in cereal samples. The developed methods were tested by analyzing 100 samples of cereals by ELISA screening for reducing costs and analysis time and then using UPLC/FLD for confirmation purposes. Both methods met the performance criteria for sensitivity, linearity, selectivity, precision, and ruggedness, as reported in the European Decision No. 2002/657/EC and in Regulation (EC) No. 401/2006. The correlation between ELISA and UPLC/FLD approaches showed good results (<i>r</i> = 0.9056), confirming that these two techniques should be considered to be complementary in the official control activities of cereal and derived products. |
| first_indexed | 2024-03-09T00:54:36Z |
| format | Article |
| id | doaj.art-84479c169c8741b1bfb09fa1c65322c9 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-09T00:54:36Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-84479c169c8741b1bfb09fa1c65322c92023-12-11T16:59:02ZengMDPI AGApplied Sciences2076-34172021-02-01114168810.3390/app11041688ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in CerealsPaola D’Agnello0Valeria Vita1Cinzia Franchino2Luigi Urbano3Antonio Curiale4Francesca Debegnach5Marco Iammarino6Giuliana Marchesani7Antonio Eugenio Chiaravalle8Rita De Pace9Chemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyAzienda Sanitaria Locale, Piazza Pavoncelli 11, 71121 Foggia, ItalyAzienda Sanitaria Locale, Piazza Pavoncelli 11, 71121 Foggia, ItalyIstituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Roma, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyChemistry Department, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, ItalyT-2 and HT-2 toxins are secondary metabolites of various species of <i>Fusarium</i>. These molecules can have high potential toxic effects for human and animal health. In this work, ELISA and ultra performance liquid chromatography with fluorescence detection (UPLC/FLD) were implemented and validated as screening and confirmatory tests for the detection of these two toxins in cereal samples. The developed methods were tested by analyzing 100 samples of cereals by ELISA screening for reducing costs and analysis time and then using UPLC/FLD for confirmation purposes. Both methods met the performance criteria for sensitivity, linearity, selectivity, precision, and ruggedness, as reported in the European Decision No. 2002/657/EC and in Regulation (EC) No. 401/2006. The correlation between ELISA and UPLC/FLD approaches showed good results (<i>r</i> = 0.9056), confirming that these two techniques should be considered to be complementary in the official control activities of cereal and derived products.https://www.mdpi.com/2076-3417/11/4/1688cerealsELISAfood safetyT-2/HT-2 toxinsUPLC-FLDvalidation |
| spellingShingle | Paola D’Agnello Valeria Vita Cinzia Franchino Luigi Urbano Antonio Curiale Francesca Debegnach Marco Iammarino Giuliana Marchesani Antonio Eugenio Chiaravalle Rita De Pace ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals Applied Sciences cereals ELISA food safety T-2/HT-2 toxins UPLC-FLD validation |
| title | ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals |
| title_full | ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals |
| title_fullStr | ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals |
| title_full_unstemmed | ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals |
| title_short | ELISA and UPLC/FLD as Screening and Confirmatory Techniques for T-2/HT-2 Mycotoxin Determination in Cereals |
| title_sort | elisa and uplc fld as screening and confirmatory techniques for t 2 ht 2 mycotoxin determination in cereals |
| topic | cereals ELISA food safety T-2/HT-2 toxins UPLC-FLD validation |
| url | https://www.mdpi.com/2076-3417/11/4/1688 |
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