Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition
ABSTRACTStreptococcus mutans is known as a contributor to dental caries. In this work, Lactobacillus pentosus MJM60383 was selected for its strong antagonistic activity against S. mutans and was characterized by good oral probiotic properties including lysozyme tolerance, adhesive ability to oral ce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2022.2161179 |
| _version_ | 1797449969475518464 |
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| author | Mingkun Gu Joo-Hyung Cho Joo-Won Suh Jinhua Cheng |
| author_facet | Mingkun Gu Joo-Hyung Cho Joo-Won Suh Jinhua Cheng |
| author_sort | Mingkun Gu |
| collection | DOAJ |
| description | ABSTRACTStreptococcus mutans is known as a contributor to dental caries. In this work, Lactobacillus pentosus MJM60383 was selected for its strong antagonistic activity against S. mutans and was characterized by good oral probiotic properties including lysozyme tolerance, adhesive ability to oral cells, good aggregation (auto-aggregation, co-aggregation) ability, hydrogen peroxide production and inhibition of biofilm formation of S. mutans. L. pentosus MJM60383 also exhibited safety as a probiotic characterized by no hemolytic activity, no D-lactate production, no biogenic amine production, and susceptibility to antibiotics. Furthermore, the biofilm formation of S. mutans was also significantly inhibited by the supernatant of L. pentosus MJM60383. An anti-biofilm mechanism study revealed that sucrose decomposition and the production of water-insoluble exopolysaccharides by S. mutans were inhibited by the treatment with L. pentosus MJM60383 supernatant. Real-time PCR analysis indicated that the supernatant of L. pentosus MJM60383 significantly inhibited the mRNA expression of S. mutans glycosyltransferases, which synthesize glucan to construct biofilm architecture and mediate bacterial adherence. Our study demonstrated L. pentosus MJM60383 as a potential oral probiotic and revealed its anti-biofilm mechanism. |
| first_indexed | 2024-03-09T14:33:51Z |
| format | Article |
| id | doaj.art-844a6df66b4e4b18a44717775a49a805 |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-03-09T14:33:51Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-844a6df66b4e4b18a44717775a49a8052023-11-27T17:25:00ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972023-12-0115110.1080/20002297.2022.2161179Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decompositionMingkun Gu0Joo-Hyung Cho1Joo-Won Suh2Jinhua Cheng3Interdisciplinary Program of Biomodulation, Myongji University, Yongin, Republic of KoreaMyongji Bioefficacy Research Center, Myongji University, Yongin, Republic of KoreaMyongji Bioefficacy Research Center, Myongji University, Yongin, Republic of KoreaMyongji Bioefficacy Research Center, Myongji University, Yongin, Republic of KoreaABSTRACTStreptococcus mutans is known as a contributor to dental caries. In this work, Lactobacillus pentosus MJM60383 was selected for its strong antagonistic activity against S. mutans and was characterized by good oral probiotic properties including lysozyme tolerance, adhesive ability to oral cells, good aggregation (auto-aggregation, co-aggregation) ability, hydrogen peroxide production and inhibition of biofilm formation of S. mutans. L. pentosus MJM60383 also exhibited safety as a probiotic characterized by no hemolytic activity, no D-lactate production, no biogenic amine production, and susceptibility to antibiotics. Furthermore, the biofilm formation of S. mutans was also significantly inhibited by the supernatant of L. pentosus MJM60383. An anti-biofilm mechanism study revealed that sucrose decomposition and the production of water-insoluble exopolysaccharides by S. mutans were inhibited by the treatment with L. pentosus MJM60383 supernatant. Real-time PCR analysis indicated that the supernatant of L. pentosus MJM60383 significantly inhibited the mRNA expression of S. mutans glycosyltransferases, which synthesize glucan to construct biofilm architecture and mediate bacterial adherence. Our study demonstrated L. pentosus MJM60383 as a potential oral probiotic and revealed its anti-biofilm mechanism.https://www.tandfonline.com/doi/10.1080/20002297.2022.2161179Lactobacillus pentosusoral probioticsbiofilmstreptococcus mutanssucrose decomposition |
| spellingShingle | Mingkun Gu Joo-Hyung Cho Joo-Won Suh Jinhua Cheng Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition Journal of Oral Microbiology Lactobacillus pentosus oral probiotics biofilm streptococcus mutans sucrose decomposition |
| title | Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| title_full | Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| title_fullStr | Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| title_full_unstemmed | Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| title_short | Potential oral probiotic Lactobacillus pentosus MJM60383 inhibits Streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| title_sort | potential oral probiotic lactobacillus pentosus mjm60383 inhibits streptococcus mutans biofilm formation by inhibiting sucrose decomposition |
| topic | Lactobacillus pentosus oral probiotics biofilm streptococcus mutans sucrose decomposition |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2022.2161179 |
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