Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function
Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones o...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-05-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/10/6/279 |
| _version_ | 1818504357845401600 |
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| author | Yaoyao Zhang Na Tang Yashar Sadigh Susan Baigent Zhiqiang Shen Venugopal Nair Yongxiu Yao |
| author_facet | Yaoyao Zhang Na Tang Yashar Sadigh Susan Baigent Zhiqiang Shen Venugopal Nair Yongxiu Yao |
| author_sort | Yaoyao Zhang |
| collection | DOAJ |
| description | Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis. |
| first_indexed | 2024-12-10T21:36:05Z |
| format | Article |
| id | doaj.art-844d57a456154748976f6273fbce446c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-10T21:36:05Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-844d57a456154748976f6273fbce446c2022-12-22T01:32:39ZengMDPI AGViruses1999-49152018-05-0110627910.3390/v10060279v10060279Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene FunctionYaoyao Zhang0Na Tang1Yashar Sadigh2Susan Baigent3Zhiqiang Shen4Venugopal Nair5Yongxiu Yao6The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKThe Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKThe Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKThe Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKBinzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases, Binzhou 256600, Shandong, ChinaThe Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKThe Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UKMarek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis.http://www.mdpi.com/1999-4915/10/6/279CRISPR/Cas9CVI988Meqpp38deletion |
| spellingShingle | Yaoyao Zhang Na Tang Yashar Sadigh Susan Baigent Zhiqiang Shen Venugopal Nair Yongxiu Yao Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function Viruses CRISPR/Cas9 CVI988 Meq pp38 deletion |
| title | Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function |
| title_full | Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function |
| title_fullStr | Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function |
| title_full_unstemmed | Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function |
| title_short | Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function |
| title_sort | application of crispr cas9 gene editing system on mdv 1 genome for the study of gene function |
| topic | CRISPR/Cas9 CVI988 Meq pp38 deletion |
| url | http://www.mdpi.com/1999-4915/10/6/279 |
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