Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway
Xiao-jie Chen,1 Shu-min Ren,2 Jian-zeng Dong,1 Chun-guang Qiu,1 Ying-wei Chen,1 Hai-long Tao1 1Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China; 2Department of Genetics and Prenatal Diagnosis, The First Affiliated Hospital of Zhengzhou Univers...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-02-01
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| Series: | Drug Design, Development and Therapy |
| Subjects: | |
| Online Access: | https://www.dovepress.com/ginkgo-biloba-extract-761-protects-myocardium-by-regulating-aktnrf2-si-peer-reviewed-article-DDDT |
| _version_ | 1831675062152855552 |
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| author | Chen X Ren S Dong J Qiu C Chen Y Tao H |
| author_facet | Chen X Ren S Dong J Qiu C Chen Y Tao H |
| author_sort | Chen X |
| collection | DOAJ |
| description | Xiao-jie Chen,1 Shu-min Ren,2 Jian-zeng Dong,1 Chun-guang Qiu,1 Ying-wei Chen,1 Hai-long Tao1 1Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China; 2Department of Genetics and Prenatal Diagnosis, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China Objective: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI).Materials and methods: Forty Sprague Dawley rats were randomly divided into following four groups: sham group, I/R group and EGb 761 groups (20 and 40 mg/kg). MIRI model was established after 14 days of administration. The myocardial infarct size and myocardial histology were measured and compared. Meanwhile, the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin T (TnT), TNF-α, IL-6, IL-1β, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were evaluated. Western blot was used to detect the expression of Caspase-3, Bax, Bcl-2, HO-1, Nrf2, Akt, p-Akt and nuclear protein Nrf2.Results: The levels of infarct size, CK-MB, LDH, TnT, TNF-α, IL-6 and IL-1β in the EGb 761 groups were significantly lower than those in the ischemia/reperfusion (I/R) group. The content of MDA was lower in the myocardium, whereas the activities of SOD and GSH-Px were higher than those in the I/R group. The expressions of Caspase-3 and Bax in the EGb 761 groups were significantly lower than those in the I/R group, whereas the expressions of Bcl-2, p-Akt and HO-1 and nuclear protein Nrf2 in the EGb 761 groups were higher than those in the I/R group.Conclusion: EGb 761 might inhibit the apoptosis of myocardial cells and protect the myocardium by activating the Akt/Nrf2 pathway, increasing the expression of HO-1, decreasing oxidative stress and repressing inflammatory reaction. Keywords: Ginkgo biloba extract, myocardial ischemia-reperfusion injury, oxidative stress, superoxide dismutase |
| first_indexed | 2024-12-20T03:56:09Z |
| format | Article |
| id | doaj.art-844f4e051b9143e9a2719aac5977fa73 |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-20T03:56:09Z |
| publishDate | 2019-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-844f4e051b9143e9a2719aac5977fa732022-12-21T19:54:18ZengDove Medical PressDrug Design, Development and Therapy1177-88812019-02-01Volume 1364765544063Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathwayChen XRen SDong JQiu CChen YTao HXiao-jie Chen,1 Shu-min Ren,2 Jian-zeng Dong,1 Chun-guang Qiu,1 Ying-wei Chen,1 Hai-long Tao1 1Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China; 2Department of Genetics and Prenatal Diagnosis, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China Objective: The aim of this study was to investigate the protective effect and mechanism of Ginkgo biloba extract-761 (EGb 761) in the rat with myocardial ischemia–reperfusion injury (MIRI).Materials and methods: Forty Sprague Dawley rats were randomly divided into following four groups: sham group, I/R group and EGb 761 groups (20 and 40 mg/kg). MIRI model was established after 14 days of administration. The myocardial infarct size and myocardial histology were measured and compared. Meanwhile, the levels of creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), troponin T (TnT), TNF-α, IL-6, IL-1β, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were evaluated. Western blot was used to detect the expression of Caspase-3, Bax, Bcl-2, HO-1, Nrf2, Akt, p-Akt and nuclear protein Nrf2.Results: The levels of infarct size, CK-MB, LDH, TnT, TNF-α, IL-6 and IL-1β in the EGb 761 groups were significantly lower than those in the ischemia/reperfusion (I/R) group. The content of MDA was lower in the myocardium, whereas the activities of SOD and GSH-Px were higher than those in the I/R group. The expressions of Caspase-3 and Bax in the EGb 761 groups were significantly lower than those in the I/R group, whereas the expressions of Bcl-2, p-Akt and HO-1 and nuclear protein Nrf2 in the EGb 761 groups were higher than those in the I/R group.Conclusion: EGb 761 might inhibit the apoptosis of myocardial cells and protect the myocardium by activating the Akt/Nrf2 pathway, increasing the expression of HO-1, decreasing oxidative stress and repressing inflammatory reaction. Keywords: Ginkgo biloba extract, myocardial ischemia-reperfusion injury, oxidative stress, superoxide dismutase https://www.dovepress.com/ginkgo-biloba-extract-761-protects-myocardium-by-regulating-aktnrf2-si-peer-reviewed-article-DDDTGinkgo biloba extractmyocardial ischemia reperfusion injuryoxidative stresssuperoxide dismutase. |
| spellingShingle | Chen X Ren S Dong J Qiu C Chen Y Tao H Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway Drug Design, Development and Therapy Ginkgo biloba extract myocardial ischemia reperfusion injury oxidative stress superoxide dismutase. |
| title | Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway |
| title_full | Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway |
| title_fullStr | Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway |
| title_full_unstemmed | Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway |
| title_short | Ginkgo biloba extract-761 protects myocardium by regulating Akt/Nrf2 signal pathway |
| title_sort | ginkgo biloba extract 761 protects myocardium by regulating akt nrf2 signal pathway |
| topic | Ginkgo biloba extract myocardial ischemia reperfusion injury oxidative stress superoxide dismutase. |
| url | https://www.dovepress.com/ginkgo-biloba-extract-761-protects-myocardium-by-regulating-aktnrf2-si-peer-reviewed-article-DDDT |
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