Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism
The ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2020-03-01
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| Series: | Horticultural Science |
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| Online Access: | https://hortsci.agriculturejournals.cz/artkey/hor-202001-0008_molecular-characterisation-of-apple-accessions-with-respect-to-aminocyclopropane-1-carboxylic-acid-synthase-gen.php |
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| author | Khethani Give Mhelembe Johan Hendrik Kriel Cecilia Bester Rouvay Roodt-Wilding Esmé Denise Louw Kenneth Richard Tobutt |
| author_facet | Khethani Give Mhelembe Johan Hendrik Kriel Cecilia Bester Rouvay Roodt-Wilding Esmé Denise Louw Kenneth Richard Tobutt |
| author_sort | Khethani Give Mhelembe |
| collection | DOAJ |
| description | The ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. 'Golden Hornet', had the b allele, which suggests it may have been introgressed from M. pumila. |
| first_indexed | 2024-04-10T08:22:52Z |
| format | Article |
| id | doaj.art-845c7501b8544206bf50a23abed0513d |
| institution | Directory Open Access Journal |
| issn | 0862-867X 1805-9333 |
| language | English |
| last_indexed | 2024-04-10T08:22:52Z |
| publishDate | 2020-03-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Horticultural Science |
| spelling | doaj.art-845c7501b8544206bf50a23abed0513d2023-02-23T03:35:26ZengCzech Academy of Agricultural SciencesHorticultural Science0862-867X1805-93332020-03-01471697910.17221/83/2018-HORTSCIhor-202001-0008Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphismKhethani Give Mhelembe0Johan Hendrik Kriel1Cecilia Bester2Rouvay Roodt-Wilding3Esmé Denise Louw4Kenneth Richard Tobutt5Agricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South AfricaAgricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South AfricaAgricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South AfricaDepartment of Genetics, Stellenbosch University, Matieland, South AfricaDepartment of Horticultural Science, Stellenbosch University, Matieland, South AfricaAgricultural Research Council Infruitec-Nietvoorbij, Stellenbosch, South AfricaThe ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. 'Golden Hornet', had the b allele, which suggests it may have been introgressed from M. pumila.https://hortsci.agriculturejournals.cz/artkey/hor-202001-0008_molecular-characterisation-of-apple-accessions-with-respect-to-aminocyclopropane-1-carboxylic-acid-synthase-gen.phpapplefruit qualitygenotypingstorabilitymalus sp.marker-assisted selection |
| spellingShingle | Khethani Give Mhelembe Johan Hendrik Kriel Cecilia Bester Rouvay Roodt-Wilding Esmé Denise Louw Kenneth Richard Tobutt Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism Horticultural Science apple fruit quality genotyping storability malus sp. marker-assisted selection |
| title | Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism |
| title_full | Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism |
| title_fullStr | Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism |
| title_full_unstemmed | Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism |
| title_short | Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism |
| title_sort | molecular characterisation of apple accessions with respect to aminocyclopropane 1 carboxylic acid synthase gene acs1 polymorphism |
| topic | apple fruit quality genotyping storability malus sp. marker-assisted selection |
| url | https://hortsci.agriculturejournals.cz/artkey/hor-202001-0008_molecular-characterisation-of-apple-accessions-with-respect-to-aminocyclopropane-1-carboxylic-acid-synthase-gen.php |
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