Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing
Qin Ni,1,2,* Xingwei Yao,3,* Jinhui Li,2,* Jinghan Ma,3,* Kaiying Wang,2 Xiong Liu,2 Peihan Li,2 Lang Yang,2 Peng Li,2 Shenlong Li1,2 1Epidemiology, Zhengzhou University, Zhengzhou, People’s Republic of China; 2Biosafety, Chinese PLA Center for Disease Control and Pre...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-12-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/genetic-and-phenotypic-characteristics-of-carbapenem-resistant-klebsie-peer-reviewed-fulltext-article-IDR |
| _version_ | 1828261692130197504 |
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| author | Ni Q Yao X Li J Ma J Wang K Liu X Li P Yang L Li P Li S |
| author_facet | Ni Q Yao X Li J Ma J Wang K Liu X Li P Yang L Li P Li S |
| author_sort | Ni Q |
| collection | DOAJ |
| description | Qin Ni,1,2,* Xingwei Yao,3,* Jinhui Li,2,* Jinghan Ma,3,* Kaiying Wang,2 Xiong Liu,2 Peihan Li,2 Lang Yang,2 Peng Li,2 Shenlong Li1,2 1Epidemiology, Zhengzhou University, Zhengzhou, People’s Republic of China; 2Biosafety, Chinese PLA Center for Disease Control and Prevention, Beijing, People’s Republic of China; 3Medical Clinical Laboratory, Dong Zhimen Hospital Beijing University of Chinese Medicine, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peng Li; Shenlong Li, Email jiekenlee@126.com; lishenlong@sohu.comObjective: Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing.Methods: Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes.Results: All strains were multi-drug resistant. Twenty-two strains carried the blaKPC-2 gene and two harbored blaNDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII (pHN7A8), IncX, or IncFIB (K).Conclusion: This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens.Keywords: Klebsiella pneumoniae, drug resistance, carbapenemase, ST11, blaKPC-2 |
| first_indexed | 2024-04-13T03:44:43Z |
| format | Article |
| id | doaj.art-845e37e2ae7a46f68586cc9479ea7a7e |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-13T03:44:43Z |
| publishDate | 2022-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-845e37e2ae7a46f68586cc9479ea7a7e2022-12-22T03:04:03ZengDove Medical PressInfection and Drug Resistance1178-69732022-12-01Volume 157503750880428Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in BeijingNi QYao XLi JMa JWang KLiu XLi PYang LLi PLi SQin Ni,1,2,* Xingwei Yao,3,* Jinhui Li,2,* Jinghan Ma,3,* Kaiying Wang,2 Xiong Liu,2 Peihan Li,2 Lang Yang,2 Peng Li,2 Shenlong Li1,2 1Epidemiology, Zhengzhou University, Zhengzhou, People’s Republic of China; 2Biosafety, Chinese PLA Center for Disease Control and Prevention, Beijing, People’s Republic of China; 3Medical Clinical Laboratory, Dong Zhimen Hospital Beijing University of Chinese Medicine, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Peng Li; Shenlong Li, Email jiekenlee@126.com; lishenlong@sohu.comObjective: Klebsiella pneumoniae is a common multidrug-resistant pathogen that jeopardizes the health of hospitalized patients. We aimed to study the phenotypic and genotypic characteristics of carbapenem-resistant K. pneumoniae (CRKP) isolates from a hospital in Beijing.Methods: Twenty-four CRKP clinical isolates were collected within a half-year to investigate antimicrobial resistance and genomic characteristics. Illumina and Nanopore sequencing were performed to assemble and annotate genomes.Results: All strains were multi-drug resistant. Twenty-two strains carried the blaKPC-2 gene and two harbored blaNDM-5. Multilocus sequence type(MLST) analysis identified five sequence types; most isolates belonged to ST11. Three strains were isolated from the same patient; each carried a different plasmid replicon, either IncFII (pHN7A8), IncX, or IncFIB (K).Conclusion: This study furthers the understanding of CRKP antimicrobial resistance genotypes, and may facilitate the control of nosocomial infections caused by antimicrobial-resistant pathogens.Keywords: Klebsiella pneumoniae, drug resistance, carbapenemase, ST11, blaKPC-2https://www.dovepress.com/genetic-and-phenotypic-characteristics-of-carbapenem-resistant-klebsie-peer-reviewed-fulltext-article-IDRklebsiella pneumoniaedrug resistancecarbapenemasest11blakpc-2 |
| spellingShingle | Ni Q Yao X Li J Ma J Wang K Liu X Li P Yang L Li P Li S Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing Infection and Drug Resistance klebsiella pneumoniae drug resistance carbapenemase st11 blakpc-2 |
| title | Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| title_full | Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| title_fullStr | Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| title_full_unstemmed | Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| title_short | Genetic and Phenotypic Characteristics of Carbapenem-Resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Beijing |
| title_sort | genetic and phenotypic characteristics of carbapenem resistant klebsiella pneumoniae isolates from a tertiary hospital in beijing |
| topic | klebsiella pneumoniae drug resistance carbapenemase st11 blakpc-2 |
| url | https://www.dovepress.com/genetic-and-phenotypic-characteristics-of-carbapenem-resistant-klebsie-peer-reviewed-fulltext-article-IDR |
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