<i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites
A novel myxobacterial strain ZKHCc1 1396<sup>T</sup> was isolated in 2017 from a soil sample collected along Chalus Road connecting Tehran and Mazandaran, Iran. It was a Gram-negative, rod-shaped bacterial strain that displayed the general features of <i>Corallococcus,</i> in...
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MDPI AG
2022-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/7/1262 |
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| author | Zahra Khosravi Babadi Ronald Garcia Gholam Hossein Ebrahimipour Chandra Risdian Peter Kämpfer Michael Jarek Rolf Müller Joachim Wink |
| author_facet | Zahra Khosravi Babadi Ronald Garcia Gholam Hossein Ebrahimipour Chandra Risdian Peter Kämpfer Michael Jarek Rolf Müller Joachim Wink |
| author_sort | Zahra Khosravi Babadi |
| collection | DOAJ |
| description | A novel myxobacterial strain ZKHCc1 1396<sup>T</sup> was isolated in 2017 from a soil sample collected along Chalus Road connecting Tehran and Mazandaran, Iran. It was a Gram-negative, rod-shaped bacterial strain that displayed the general features of <i>Corallococcus,</i> including gliding and fruiting body formation on agar and microbial lytic activity. Strain ZKHCc1 1396<sup>T</sup> was characterized as an aerobic, mesophilic, and chemoheterotrophic bacterium resistant to many antibiotics. The major cellular fatty acids were branched-chain <i>iso</i>-C<sub>17:0</sub> 2-OH, <i>iso</i>-C<sub>15:0</sub>, <i>iso</i>-C<sub>17:1</sub>, and <i>iso</i>-C<sub>17:0</sub>. The strain showed the highest 16S rRNA gene sequence similarity to <i>Corallococcus</i><i>terminator</i> CA054A<sup>T</sup> (99.67%) and <i>C. praedator</i> CA031B<sup>T</sup> (99.17%), and formed a novel branch both in the 16S rRNA gene sequence and phylogenomic tree. The genome size was 9,437,609 bp, with a DNA G + C content of 69.8 mol%. The strain had an average nucleotide identity (ANI) value lower than the species cut-off (95%), and with the digital DNA–DNA hybridization (dDDH) below the 70% threshold compared to the closest type strains. Secondary metabolite and biosynthetic gene cluster analyses revealed the strain’s potential to produce novel compounds. Based on polyphasic taxonomic characterization, we propose that strain ZKHCc1 1396<sup>T</sup> represents a novel species, <i>Corallococcus soli</i> sp. nov. (NCCB 100659<sup>T</sup> = CIP 111634<sup>T</sup>). |
| first_indexed | 2024-03-09T06:11:41Z |
| format | Article |
| id | doaj.art-845efff725734830a0d821966119ed27 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T06:11:41Z |
| publishDate | 2022-06-01 |
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| series | Microorganisms |
| spelling | doaj.art-845efff725734830a0d821966119ed272023-12-03T11:58:26ZengMDPI AGMicroorganisms2076-26072022-06-01107126210.3390/microorganisms10071262<i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary MetabolitesZahra Khosravi Babadi0Ronald Garcia1Gholam Hossein Ebrahimipour2Chandra Risdian3Peter Kämpfer4Michael Jarek5Rolf Müller6Joachim Wink7Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University GC, Tehran 1983969411, IranHelmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University, Campus E8 1, 66123 Saarbrücken, GermanyDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University GC, Tehran 1983969411, IranMicrobial Strain Collection, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment of Applied Microbiology, Justus Liebig University Gießen, 35392 Gießen, GermanyGenome Analytics, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyHelmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University, Campus E8 1, 66123 Saarbrücken, GermanyMicrobial Strain Collection, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyA novel myxobacterial strain ZKHCc1 1396<sup>T</sup> was isolated in 2017 from a soil sample collected along Chalus Road connecting Tehran and Mazandaran, Iran. It was a Gram-negative, rod-shaped bacterial strain that displayed the general features of <i>Corallococcus,</i> including gliding and fruiting body formation on agar and microbial lytic activity. Strain ZKHCc1 1396<sup>T</sup> was characterized as an aerobic, mesophilic, and chemoheterotrophic bacterium resistant to many antibiotics. The major cellular fatty acids were branched-chain <i>iso</i>-C<sub>17:0</sub> 2-OH, <i>iso</i>-C<sub>15:0</sub>, <i>iso</i>-C<sub>17:1</sub>, and <i>iso</i>-C<sub>17:0</sub>. The strain showed the highest 16S rRNA gene sequence similarity to <i>Corallococcus</i><i>terminator</i> CA054A<sup>T</sup> (99.67%) and <i>C. praedator</i> CA031B<sup>T</sup> (99.17%), and formed a novel branch both in the 16S rRNA gene sequence and phylogenomic tree. The genome size was 9,437,609 bp, with a DNA G + C content of 69.8 mol%. The strain had an average nucleotide identity (ANI) value lower than the species cut-off (95%), and with the digital DNA–DNA hybridization (dDDH) below the 70% threshold compared to the closest type strains. Secondary metabolite and biosynthetic gene cluster analyses revealed the strain’s potential to produce novel compounds. Based on polyphasic taxonomic characterization, we propose that strain ZKHCc1 1396<sup>T</sup> represents a novel species, <i>Corallococcus soli</i> sp. nov. (NCCB 100659<sup>T</sup> = CIP 111634<sup>T</sup>).https://www.mdpi.com/2076-2607/10/7/1262myxobacteriamyxococcales<i>Corallococcus</i><i>Corallococcus soli</i> |
| spellingShingle | Zahra Khosravi Babadi Ronald Garcia Gholam Hossein Ebrahimipour Chandra Risdian Peter Kämpfer Michael Jarek Rolf Müller Joachim Wink <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites Microorganisms myxobacteria myxococcales <i>Corallococcus</i> <i>Corallococcus soli</i> |
| title | <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites |
| title_full | <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites |
| title_fullStr | <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites |
| title_full_unstemmed | <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites |
| title_short | <i>Corallococcus soli</i> sp. Nov., a Soil Myxobacterium Isolated from Subtropical Climate, Chalus County, Iran, and Its Potential to Produce Secondary Metabolites |
| title_sort | i corallococcus soli i sp nov a soil myxobacterium isolated from subtropical climate chalus county iran and its potential to produce secondary metabolites |
| topic | myxobacteria myxococcales <i>Corallococcus</i> <i>Corallococcus soli</i> |
| url | https://www.mdpi.com/2076-2607/10/7/1262 |
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