Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro
<i>Sorbaria pallasii</i> is an endemic species of the Far East and Siberia and grows along the Goltsy altitudinal belt. Data on micropropagation and phytochemical characteristics of this plant are not available, probably because of the inaccessibility of the plant material. Morphogenesis...
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MDPI AG
2023-02-01
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| Series: | Life |
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| author | Titiana V. Zheleznichenko Tatiana N. Veklich Vera A. Kostikova |
| author_facet | Titiana V. Zheleznichenko Tatiana N. Veklich Vera A. Kostikova |
| author_sort | Titiana V. Zheleznichenko |
| collection | DOAJ |
| description | <i>Sorbaria pallasii</i> is an endemic species of the Far East and Siberia and grows along the Goltsy altitudinal belt. Data on micropropagation and phytochemical characteristics of this plant are not available, probably because of the inaccessibility of the plant material. Morphogenesis initiation from flower buds of <i>S. pallasii</i> in vitro and micropropagation were performed here in the Murashige and Skoog medium supplemented with 5.0 µM 6-benzylaminopurine and 0.0–1.0 µM α-naphthylacetic acid; elongation was implemented in the same medium without the hormones. A well-growing sterile culture of <i>S. pallasii</i> was obtained; the number of microshoots per explant reached 5.7 ± 1.2. Phytochemical analyses of in vitro propagated <i>S. pallasii</i> detected 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in a water-ethanol extract from its microshoots and revealed phenolic compounds in it. The phenolic compounds that likely contribute to its biological activity are tannins (74.9 mg/g), phenolcarboxylic acids (30.8 mg/g), and catechins (13.3 mg/g). In the microshoot extract, high-performance liquid chromatography identified three catechins. Microshoots showed the highest concentration of (±)-catechin (3.03 mg/(g of absolutely dry mass; ADM)). Concentrations of epigallocatechin gallate (0.38 mg/(g of ADM)) and (−)-epicatechin (0.55 mg/(g of ADM)) were significantly lower. This study paves the way for further biotechnological and phytochemical research on <i>S. pallasii</i>. |
| first_indexed | 2024-03-11T08:32:34Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T08:32:34Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-8460387d65c444f18d8856fbf40a546f2023-11-16T21:42:54ZengMDPI AGLife2075-17292023-02-0113255710.3390/life13020557Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In VitroTitiana V. Zheleznichenko0Tatiana N. Veklich1Vera A. Kostikova2Central Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences (CSBG SB RAS), 630090 Novosibirsk, RussiaAmur Branch of Botanical Garden-Institute, Far Eastern Branch of Russian Academy of Sciences, 675000 Blagoveshchensk, RussiaCentral Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences (CSBG SB RAS), 630090 Novosibirsk, Russia<i>Sorbaria pallasii</i> is an endemic species of the Far East and Siberia and grows along the Goltsy altitudinal belt. Data on micropropagation and phytochemical characteristics of this plant are not available, probably because of the inaccessibility of the plant material. Morphogenesis initiation from flower buds of <i>S. pallasii</i> in vitro and micropropagation were performed here in the Murashige and Skoog medium supplemented with 5.0 µM 6-benzylaminopurine and 0.0–1.0 µM α-naphthylacetic acid; elongation was implemented in the same medium without the hormones. A well-growing sterile culture of <i>S. pallasii</i> was obtained; the number of microshoots per explant reached 5.7 ± 1.2. Phytochemical analyses of in vitro propagated <i>S. pallasii</i> detected 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity in a water-ethanol extract from its microshoots and revealed phenolic compounds in it. The phenolic compounds that likely contribute to its biological activity are tannins (74.9 mg/g), phenolcarboxylic acids (30.8 mg/g), and catechins (13.3 mg/g). In the microshoot extract, high-performance liquid chromatography identified three catechins. Microshoots showed the highest concentration of (±)-catechin (3.03 mg/(g of absolutely dry mass; ADM)). Concentrations of epigallocatechin gallate (0.38 mg/(g of ADM)) and (−)-epicatechin (0.55 mg/(g of ADM)) were significantly lower. This study paves the way for further biotechnological and phytochemical research on <i>S. pallasii</i>.https://www.mdpi.com/2075-1729/13/2/557<i>Sorbaria pallasii</i>HPLCcatechinphenolcarboxylic acidtanninflavonoid |
| spellingShingle | Titiana V. Zheleznichenko Tatiana N. Veklich Vera A. Kostikova Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro Life <i>Sorbaria pallasii</i> HPLC catechin phenolcarboxylic acid tannin flavonoid |
| title | Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro |
| title_full | Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro |
| title_fullStr | Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro |
| title_full_unstemmed | Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro |
| title_short | Investigation of Phenolic Compounds and Antioxidant Activity of <i>Sorbaria pallasii</i> (Rosaceae) Microshoots Grown In Vitro |
| title_sort | investigation of phenolic compounds and antioxidant activity of i sorbaria pallasii i rosaceae microshoots grown in vitro |
| topic | <i>Sorbaria pallasii</i> HPLC catechin phenolcarboxylic acid tannin flavonoid |
| url | https://www.mdpi.com/2075-1729/13/2/557 |
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