Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description
Abstract With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the py...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-05-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.735 |
| _version_ | 1819071835274215424 |
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| author | Melhem Bilen Maxime D. Mbogning Fonkou Thi T. Nguyen Magali Richez Ziad Daoud Pierre E. Fournier Didier Raoult Frédéric Cadoret |
| author_facet | Melhem Bilen Maxime D. Mbogning Fonkou Thi T. Nguyen Magali Richez Ziad Daoud Pierre E. Fournier Didier Raoult Frédéric Cadoret |
| author_sort | Melhem Bilen |
| collection | DOAJ |
| description | Abstract With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678T has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille‐P4678T. |
| first_indexed | 2024-12-21T17:28:08Z |
| format | Article |
| id | doaj.art-84638ae779584df5a5975b33a1d55de1 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-21T17:28:08Z |
| publishDate | 2019-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-84638ae779584df5a5975b33a1d55de12022-12-21T18:55:59ZengWileyMicrobiologyOpen2045-88272019-05-0185n/an/a10.1002/mbo3.735Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics descriptionMelhem Bilen0Maxime D. Mbogning Fonkou1Thi T. Nguyen2Magali Richez3Ziad Daoud4Pierre E. Fournier5Didier Raoult6Frédéric Cadoret7Aix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceClinical Microbiology Department Faculty of Medicine and Medical Sciences University of Balamand Amioun LebanonAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAix Marseille Univ IRD, APHM MEPHI IHU‐Méditerranée Infection Marseille FranceAbstract With the aim of describing the human microbiota by the means of culture methods, culturomics was developed in order to target previously un‐isolated bacterial species and describe it via the taxono‐genomics approach. While performing a descriptive study of the human gut microbiota of the pygmy people, strain Marseille‐P4678T has been isolated from a stool sample of a healthy 39‐year‐old pygmy male. Cells of this strain were Gram‐positive cocci, spore‐forming, non‐motile, catalase‐positive and oxidase‐negative, and grow optimally at 37°C under anaerobic conditions. Its 16S rRNA gene sequence exhibited 89.69% of sequence similarity with Parvimonas micra strain 3119BT (NR 036934.1), its phylogenetically closest species with standing in nomenclature. The genome of strain Marseille‐P4678T is 2,083,161 long with 28.26 mol% of G+C content. Based on its phenotypic, biochemical, genotypic and proteomic profile, this bacterium was classified as a new bacterial genus and species Miniphocibacter massiliensis gen. nov., sp. nov. with the type strain Marseille‐P4678T.https://doi.org/10.1002/mbo3.735culturomicsgut microbiotaMiniphocibacter massiliensisnew speciespygmytaxono‐genomics |
| spellingShingle | Melhem Bilen Maxime D. Mbogning Fonkou Thi T. Nguyen Magali Richez Ziad Daoud Pierre E. Fournier Didier Raoult Frédéric Cadoret Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description MicrobiologyOpen culturomics gut microbiota Miniphocibacter massiliensis new species pygmy taxono‐genomics |
| title | Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description |
| title_full | Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description |
| title_fullStr | Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description |
| title_full_unstemmed | Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description |
| title_short | Miniphocibacter massiliensis gen. nov., sp. nov., a new species isolated from the human gut and its taxono‐genomics description |
| title_sort | miniphocibacter massiliensis gen nov sp nov a new species isolated from the human gut and its taxono genomics description |
| topic | culturomics gut microbiota Miniphocibacter massiliensis new species pygmy taxono‐genomics |
| url | https://doi.org/10.1002/mbo3.735 |
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