Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome
A complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In thi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01187/full |
| _version_ | 1819298480166797312 |
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| author | Svetlana N. Yurgel Gavin M. Douglas Ashley Dusault David Percival Morgan G. I. Langille |
| author_facet | Svetlana N. Yurgel Gavin M. Douglas Ashley Dusault David Percival Morgan G. I. Langille |
| author_sort | Svetlana N. Yurgel |
| collection | DOAJ |
| description | A complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In this study, we employed a unique wild blueberry ecosystem to evaluate plant-associated microbiota, encompassing both eukaryotic and bacterial communities. We found that, while soil microbiome serves as a foundation for root microbiome, plant-influenced species sorting had stronger effect on eukaryotes than on bacteria. Our study identified several fungal and protist taxa, which are correlated with decreased fruit production in wild blueberry agricultural ecosystems. The specific effect of species sorting in root microbiome resulted in an increase in relative abundance of fungi adapted to plant-associated life-style, while the relative abundance of non-fungal eukaryotes was decreased along the soil-endosphere continuum in the root, probably because of low adaptation of these microorganisms to host–plant defense responses. Analysis of community correlation networks indicated that bacterial and eukaryotic interactions became more complex along the soil-endosphere continuum and, in addition to extensive mutualistic interactions, co-exclusion also played an important role in shaping wild blueberry associated microbiome. Our study identified several potential hub taxa with important roles in soil fertility and/or plant–microbe interaction, suggesting the key role of these taxa in the interconnection between soils and plant health and overall microbial community structure. This study also provides a comprehensive view of the role of non-fungal eukaryotes in soil ecosystem. |
| first_indexed | 2024-12-24T05:30:34Z |
| format | Article |
| id | doaj.art-846536a8aa364fc3a4a5fd446e4680eb |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-24T05:30:34Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-846536a8aa364fc3a4a5fd446e4680eb2022-12-21T17:13:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-06-01910.3389/fmicb.2018.01187364508Dissecting Community Structure in Wild Blueberry Root and Soil MicrobiomeSvetlana N. Yurgel0Gavin M. Douglas1Ashley Dusault2David Percival3Morgan G. I. Langille4Department of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, CanadaDepartment of Microbiology and Immunology, Dalhousie University, Halifax, NS, CanadaDepartment of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, CanadaDepartment of Plant, Food, and Environmental Sciences, Dalhousie University, Truro, NS, CanadaDepartment of Pharmacology, Dalhousie University, Halifax, NS, CanadaA complex network of functions and symbiotic interactions between a eukaryotic host and its microbiome is a the foundation of the ecological unit holobiont. However, little is known about how the non-fungal eukaryotic microorganisms fit in this complex network of host–microbiome interactions. In this study, we employed a unique wild blueberry ecosystem to evaluate plant-associated microbiota, encompassing both eukaryotic and bacterial communities. We found that, while soil microbiome serves as a foundation for root microbiome, plant-influenced species sorting had stronger effect on eukaryotes than on bacteria. Our study identified several fungal and protist taxa, which are correlated with decreased fruit production in wild blueberry agricultural ecosystems. The specific effect of species sorting in root microbiome resulted in an increase in relative abundance of fungi adapted to plant-associated life-style, while the relative abundance of non-fungal eukaryotes was decreased along the soil-endosphere continuum in the root, probably because of low adaptation of these microorganisms to host–plant defense responses. Analysis of community correlation networks indicated that bacterial and eukaryotic interactions became more complex along the soil-endosphere continuum and, in addition to extensive mutualistic interactions, co-exclusion also played an important role in shaping wild blueberry associated microbiome. Our study identified several potential hub taxa with important roles in soil fertility and/or plant–microbe interaction, suggesting the key role of these taxa in the interconnection between soils and plant health and overall microbial community structure. This study also provides a comprehensive view of the role of non-fungal eukaryotes in soil ecosystem.https://www.frontiersin.org/article/10.3389/fmicb.2018.01187/fullbacterial communitieseukaryotic communitiesplant–microbe interactionVaccinium angustifoliumcommunity networks |
| spellingShingle | Svetlana N. Yurgel Gavin M. Douglas Ashley Dusault David Percival Morgan G. I. Langille Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome Frontiers in Microbiology bacterial communities eukaryotic communities plant–microbe interaction Vaccinium angustifolium community networks |
| title | Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome |
| title_full | Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome |
| title_fullStr | Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome |
| title_full_unstemmed | Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome |
| title_short | Dissecting Community Structure in Wild Blueberry Root and Soil Microbiome |
| title_sort | dissecting community structure in wild blueberry root and soil microbiome |
| topic | bacterial communities eukaryotic communities plant–microbe interaction Vaccinium angustifolium community networks |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01187/full |
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