Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model
Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2016-07-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/096368915X689730 |
| _version_ | 1818402300305080320 |
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| author | Lukas Skoloudik Viktor Chrobok David Kalfert Zuzana Koci Eva Sykova Tetyana Chumak Jiri Popelar Josef Syka Jan Laco Jana Dedková Govindan Dayanithi Stanislav Filip M.D., Ph.D., D.Sc. |
| author_facet | Lukas Skoloudik Viktor Chrobok David Kalfert Zuzana Koci Eva Sykova Tetyana Chumak Jiri Popelar Josef Syka Jan Laco Jana Dedková Govindan Dayanithi Stanislav Filip M.D., Ph.D., D.Sc. |
| author_sort | Lukas Skoloudik |
| collection | DOAJ |
| description | Canal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation ( p = 0.00174), as well as a significantly higher volume percentage of new immature bone ( p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation. |
| first_indexed | 2024-12-14T08:06:10Z |
| format | Article |
| id | doaj.art-8466ada4b4ba4df9892ff0ecb08855d4 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-14T08:06:10Z |
| publishDate | 2016-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-8466ada4b4ba4df9892ff0ecb08855d42022-12-21T23:10:12ZengSAGE PublishingCell Transplantation0963-68971555-38922016-07-012510.3727/096368915X689730Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal ModelLukas Skoloudik0Viktor Chrobok1David Kalfert2Zuzana Koci3Eva Sykova4Tetyana Chumak5Jiri Popelar6Josef Syka7Jan Laco8Jana Dedková9Govindan Dayanithi10Stanislav Filip M.D., Ph.D., D.Sc.11 Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic Department of Otorhinolaryngology and Head and Neck Surgery, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic Bioinova, Ltd., Prague, Czech Republic Department of Neuroscience, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic The Fingerland Department of Pathology, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic Department of Radiology, University Hospital Hradec Kralové, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech Republic Ecole Pratique des Hautes Etudes-Sorbonne, Paris, France Department of Oncology and Radiotherapy, Charles University in Prague, Faculty of Medicine in Hradec Kralové, Hradec Kralové, Czech RepublicCanal wall down mastoidectomy is one of the most effective treatments for cholesteatoma. However, it results in anatomical changes in the external and middle ear with a negative impact on the patient's quality of life. To provide complete closure of the mastoid cavity and normalize the anatomy of the middle and external ear, we used human multipotent mesenchymal stromal cells (hMSCs), GMP grade, in a guinea pig model. A method for preparing a biomaterial composed of hMSCs, hydroxyapatite, and tissue glue was developed. Animals from the treated group were implanted with biomaterial composed of hydroxyapatite and hMSCs, while animals in the control group received hydroxyapatite alone. When compared to controls, the group implanted with hMSCs showed a significantly higher ratio of new bone formation ( p = 0.00174), as well as a significantly higher volume percentage of new immature bone ( p = 0.00166). Our results proved a beneficial effect of hMSCs on temporal bone formation and provided a promising tool to improve the quality of life of patients after canal wall down mastoidectomy by hMSC implantation.https://doi.org/10.3727/096368915X689730 |
| spellingShingle | Lukas Skoloudik Viktor Chrobok David Kalfert Zuzana Koci Eva Sykova Tetyana Chumak Jiri Popelar Josef Syka Jan Laco Jana Dedková Govindan Dayanithi Stanislav Filip M.D., Ph.D., D.Sc. Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model Cell Transplantation |
| title | Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model |
| title_full | Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model |
| title_fullStr | Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model |
| title_full_unstemmed | Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model |
| title_short | Human Multipotent Mesenchymal Stromal Cells in the Treatment of Postoperative Temporal Bone Defect: An Animal Model |
| title_sort | human multipotent mesenchymal stromal cells in the treatment of postoperative temporal bone defect an animal model |
| url | https://doi.org/10.3727/096368915X689730 |
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