Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design
This study reports on the optimization of the extraction conditions of cordycepin from Cordyceps militaris by using ultrasonication. For this purpose, the orthogonal experimental design was used to investigate the effects of factors on the ultrasonic-assisted extraction (UAE). Four factors: extracti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/19/12/20808 |
| _version_ | 1819137337970393088 |
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| author | Hsiu-Ju Wang Meng-Chun Pan Chao-Kai Chang Shu-Wei Chang Chang-Wei Hsieh |
| author_facet | Hsiu-Ju Wang Meng-Chun Pan Chao-Kai Chang Shu-Wei Chang Chang-Wei Hsieh |
| author_sort | Hsiu-Ju Wang |
| collection | DOAJ |
| description | This study reports on the optimization of the extraction conditions of cordycepin from Cordyceps militaris by using ultrasonication. For this purpose, the orthogonal experimental design was used to investigate the effects of factors on the ultrasonic-assisted extraction (UAE). Four factors: extraction time (min), ethanol concentration (%), extraction temperature (°C) and extraction frequency (kHz), were studied. The results showed that the highest cordycepin yield of 7.04 mg/g (86.98% ± 0.23%) was obtained with an extraction time of 60 min, ethanol concentration of 50%, extraction temperature of 65 °C and extraction frequency of 56 kHz. It was found that the cordycepin extraction yield increased with the effect of ultrasonication during the extraction process. Therefore, UAE can be used as an alternative to conventional immersion extraction with respect to the recovery of cordycepin from C. militaris, with the advantages of shorter extraction time and reduced solvent consumption. |
| first_indexed | 2024-12-22T10:49:17Z |
| format | Article |
| id | doaj.art-8468b8c7340f432bbff33050adfaa07e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-22T10:49:17Z |
| publishDate | 2014-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-8468b8c7340f432bbff33050adfaa07e2022-12-21T18:28:50ZengMDPI AGMolecules1420-30492014-12-011912208082082010.3390/molecules191220808molecules191220808Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental DesignHsiu-Ju Wang0Meng-Chun Pan1Chao-Kai Chang2Shu-Wei Chang3Chang-Wei Hsieh4Department of Hospitality Management, Mingdao University, 369 Wen-Hua Rd, Peetow, Chang-Hua 52345, TaiwanDepartment of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, TaiwanDepartment of BioIndustry Technology, Da-Yeh University, 168 University Rd, Dacun, Chang-Hua 51591, TaiwanDepartment of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, TaiwanDepartment of Medicinal Botanicals and Health Applications, Da-Yen University, 168 University Rd, Dacun, Chang-Hua 51591, TaiwanThis study reports on the optimization of the extraction conditions of cordycepin from Cordyceps militaris by using ultrasonication. For this purpose, the orthogonal experimental design was used to investigate the effects of factors on the ultrasonic-assisted extraction (UAE). Four factors: extraction time (min), ethanol concentration (%), extraction temperature (°C) and extraction frequency (kHz), were studied. The results showed that the highest cordycepin yield of 7.04 mg/g (86.98% ± 0.23%) was obtained with an extraction time of 60 min, ethanol concentration of 50%, extraction temperature of 65 °C and extraction frequency of 56 kHz. It was found that the cordycepin extraction yield increased with the effect of ultrasonication during the extraction process. Therefore, UAE can be used as an alternative to conventional immersion extraction with respect to the recovery of cordycepin from C. militaris, with the advantages of shorter extraction time and reduced solvent consumption.http://www.mdpi.com/1420-3049/19/12/20808ultrasonic-assisted extractionCordyceps militariscordycepinorthogonal experimental design |
| spellingShingle | Hsiu-Ju Wang Meng-Chun Pan Chao-Kai Chang Shu-Wei Chang Chang-Wei Hsieh Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design Molecules ultrasonic-assisted extraction Cordyceps militaris cordycepin orthogonal experimental design |
| title | Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design |
| title_full | Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design |
| title_fullStr | Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design |
| title_full_unstemmed | Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design |
| title_short | Optimization of Ultrasonic-Assisted Extraction of Cordycepin from Cordyceps militaris Using Orthogonal Experimental Design |
| title_sort | optimization of ultrasonic assisted extraction of cordycepin from cordyceps militaris using orthogonal experimental design |
| topic | ultrasonic-assisted extraction Cordyceps militaris cordycepin orthogonal experimental design |
| url | http://www.mdpi.com/1420-3049/19/12/20808 |
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