Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling
Targeted metabolomic analysis was conducted on juvenile Chinook salmon exposed for 10 days to wastewater effluent (WWE) from a large urban treatment plant. Exposures included five dilutions of WWE (20%, 5.3%, 1.4%, 0.4%, and 0.1%) and a control with 7 replicates per treatment. Liver was extracted fr...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-05-01
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| Series: | Aquaculture and Fisheries |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X23000916 |
| _version_ | 1797234049950941184 |
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| author | James P. Meador Suzanne C. Ball C. Andrew James Jenifer K. McIntyre |
| author_facet | James P. Meador Suzanne C. Ball C. Andrew James Jenifer K. McIntyre |
| author_sort | James P. Meador |
| collection | DOAJ |
| description | Targeted metabolomic analysis was conducted on juvenile Chinook salmon exposed for 10 days to wastewater effluent (WWE) from a large urban treatment plant. Exposures included five dilutions of WWE (20%, 5.3%, 1.4%, 0.4%, and 0.1%) and a control with 7 replicates per treatment. Liver was extracted from fish and analyzed via liquid chromatography – mass spectrometry (LC-MS) for 361 endogenous metabolites, of which 185 were detected. Control-versus-treatment comparisons identified several metabolites that were associated with altered biochemical pathways observed for all treatments, including several that are important for energy generation and utilization. These altered pathways are crucial for fish health and may be an early indicator of potential adverse effects on growth, reproduction, behavior, and immune function. Juvenile ocean-type Chinook salmon spend several days to weeks in the nearshore estuary where they may encounter high concentrations of WWE contaminants. They are exposed to a wide range of potent pharmaceuticals, personal care products, and industrial compounds from WWE that have the potential to affect physiological homeostasis and disrupt their normal life cycle. |
| first_indexed | 2024-04-24T16:25:53Z |
| format | Article |
| id | doaj.art-846b5de14e364d0dbefd00d17f196c05 |
| institution | Directory Open Access Journal |
| issn | 2468-550X |
| language | English |
| last_indexed | 2024-04-24T16:25:53Z |
| publishDate | 2024-05-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Aquaculture and Fisheries |
| spelling | doaj.art-846b5de14e364d0dbefd00d17f196c052024-03-31T04:37:53ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2024-05-0193367377Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profilingJames P. Meador0Suzanne C. Ball1C. Andrew James2Jenifer K. McIntyre3Dept. of Environmental and Occupational Health Sciences, School of Public Health, 4225 Roosevelt Way NE, Suite 100, Seattle, WA, 98105–6099, USA; Corresponding author.Washington State University, School of the Environment, Puyallup Research and Extension Center, 2606 W Pioneer Ave, Puyallup, WA, 98371, USAUniversity of Washington Tacoma, Center for Urban Waters, 326 East D Street, Tacoma, WA, 98421-1801, USAWashington State University, School of the Environment, Puyallup Research and Extension Center, 2606 W Pioneer Ave, Puyallup, WA, 98371, USATargeted metabolomic analysis was conducted on juvenile Chinook salmon exposed for 10 days to wastewater effluent (WWE) from a large urban treatment plant. Exposures included five dilutions of WWE (20%, 5.3%, 1.4%, 0.4%, and 0.1%) and a control with 7 replicates per treatment. Liver was extracted from fish and analyzed via liquid chromatography – mass spectrometry (LC-MS) for 361 endogenous metabolites, of which 185 were detected. Control-versus-treatment comparisons identified several metabolites that were associated with altered biochemical pathways observed for all treatments, including several that are important for energy generation and utilization. These altered pathways are crucial for fish health and may be an early indicator of potential adverse effects on growth, reproduction, behavior, and immune function. Juvenile ocean-type Chinook salmon spend several days to weeks in the nearshore estuary where they may encounter high concentrations of WWE contaminants. They are exposed to a wide range of potent pharmaceuticals, personal care products, and industrial compounds from WWE that have the potential to affect physiological homeostasis and disrupt their normal life cycle.http://www.sciencedirect.com/science/article/pii/S2468550X23000916Wastewater effluentMetabolomicsPhysiologyChinook salmonPharmaceuticalsMetabolic pathways |
| spellingShingle | James P. Meador Suzanne C. Ball C. Andrew James Jenifer K. McIntyre Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling Aquaculture and Fisheries Wastewater effluent Metabolomics Physiology Chinook salmon Pharmaceuticals Metabolic pathways |
| title | Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling |
| title_full | Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling |
| title_fullStr | Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling |
| title_full_unstemmed | Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling |
| title_short | Exposure of juvenile Chinook salmon to effluent from a large urban wastewater treatment plant. Part 2. Metabolomic profiling |
| title_sort | exposure of juvenile chinook salmon to effluent from a large urban wastewater treatment plant part 2 metabolomic profiling |
| topic | Wastewater effluent Metabolomics Physiology Chinook salmon Pharmaceuticals Metabolic pathways |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X23000916 |
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