<b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L.
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted i...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2015-03-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/23119 |
| _version_ | 1818235855177777152 |
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| author | Gleuvânia Santana Marques Josefa Janaína do Anjos Sousa Ana Paula Peron |
| author_facet | Gleuvânia Santana Marques Josefa Janaína do Anjos Sousa Ana Paula Peron |
| author_sort | Gleuvânia Santana Marques |
| collection | DOAJ |
| description | This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p < 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic. |
| first_indexed | 2024-12-12T12:00:36Z |
| format | Article |
| id | doaj.art-846dfd48b1ff4d889a543d2a8b40dc35 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-12-12T12:00:36Z |
| publishDate | 2015-03-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-846dfd48b1ff4d889a543d2a8b40dc352022-12-22T00:25:07ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2015-03-0137110110610.4025/actascibiolsci.v37i1.2311911740<b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L.Gleuvânia Santana Marques0Josefa Janaína do Anjos Sousa1Ana Paula Peron2Universidade Federal do PiauíUniversidade Federal do PiauíUniversidade Federal do PiauíThis study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p < 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic.http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/23119azo dyecell divisioncellular aberrationscytotoxicity |
| spellingShingle | Gleuvânia Santana Marques Josefa Janaína do Anjos Sousa Ana Paula Peron <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. Acta Scientiarum: Biological Sciences azo dye cell division cellular aberrations cytotoxicity |
| title | <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. |
| title_full | <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. |
| title_fullStr | <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. |
| title_full_unstemmed | <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. |
| title_short | <b>Action of Ponceau 4R (E-124) food dye on root meristematic cells of <i>Allium cepa</i> L. |
| title_sort | b action of ponceau 4r e 124 food dye on root meristematic cells of i allium cepa i l |
| topic | azo dye cell division cellular aberrations cytotoxicity |
| url | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/23119 |
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