Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition
Artificial reconstruction of three-dimensional (3D) hydrogel microstructures would greatly contribute to tissue assembly in vitro, and has been widely applied in tissue engineering and drug screening. Recent technological advances in the assembly of functional hydrogel microstructures such as microf...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-06-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/8/6/192 |
| _version_ | 1811242223517302784 |
|---|---|
| author | Yang Liu Cong Wu Hok Sum Sam Lai Yan Ting Liu Wen Jung Li Ya Jing Shen |
| author_facet | Yang Liu Cong Wu Hok Sum Sam Lai Yan Ting Liu Wen Jung Li Ya Jing Shen |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Artificial reconstruction of three-dimensional (3D) hydrogel microstructures would greatly contribute to tissue assembly in vitro, and has been widely applied in tissue engineering and drug screening. Recent technological advances in the assembly of functional hydrogel microstructures such as microfluidic, 3D bioprinting, and micromold-based 3D hydrogel fabrication methods have enabled the formation of 3D tissue constructs. However, they still lack flexibility and high efficiency, which restrict their application in 3D tissue constructs. Alternatively, we report a feasible method for the fabrication and reconstruction of customized 3D hydrogel blocks. Arbitrary hydrogel microstructures were fabricated in situ via flexible and rapid light-addressable electrodeposition. To demonstrate the versatility of this method, the higher-order assembly of 3D hydrogel blocks was investigated using a constant direct current (DC) voltage (6 V) applied between two electrodes for 20–120 s. In addition to the plane-based two-dimensional (2D) assembly, hierarchical structures—including multi-layer 3D hydrogel structures and vessel-shaped structures—could be assembled using the proposed method. Overall, we developed a platform that enables researchers to construct complex 3D hydrogel microstructures efficiently and simply, which has the potential to facilitate research on drug screening and 3D tissue constructs. |
| first_indexed | 2024-04-12T13:47:30Z |
| format | Article |
| id | doaj.art-8473c33ceed642939f9f046ac2ea2e41 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-12T13:47:30Z |
| publishDate | 2017-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-8473c33ceed642939f9f046ac2ea2e412022-12-22T03:30:37ZengMDPI AGMicromachines2072-666X2017-06-018619210.3390/mi8060192mi8060192Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable ElectrodepositionYang Liu0Cong Wu1Hok Sum Sam Lai2Yan Ting Liu3Wen Jung Li4Ya Jing Shen5Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaDepartment of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong, ChinaArtificial reconstruction of three-dimensional (3D) hydrogel microstructures would greatly contribute to tissue assembly in vitro, and has been widely applied in tissue engineering and drug screening. Recent technological advances in the assembly of functional hydrogel microstructures such as microfluidic, 3D bioprinting, and micromold-based 3D hydrogel fabrication methods have enabled the formation of 3D tissue constructs. However, they still lack flexibility and high efficiency, which restrict their application in 3D tissue constructs. Alternatively, we report a feasible method for the fabrication and reconstruction of customized 3D hydrogel blocks. Arbitrary hydrogel microstructures were fabricated in situ via flexible and rapid light-addressable electrodeposition. To demonstrate the versatility of this method, the higher-order assembly of 3D hydrogel blocks was investigated using a constant direct current (DC) voltage (6 V) applied between two electrodes for 20–120 s. In addition to the plane-based two-dimensional (2D) assembly, hierarchical structures—including multi-layer 3D hydrogel structures and vessel-shaped structures—could be assembled using the proposed method. Overall, we developed a platform that enables researchers to construct complex 3D hydrogel microstructures efficiently and simply, which has the potential to facilitate research on drug screening and 3D tissue constructs.http://www.mdpi.com/2072-666X/8/6/192three-dimensional (3D) hydrogel assemblyTiOPcalginate hydrogellight-induced electrodeposition |
| spellingShingle | Yang Liu Cong Wu Hok Sum Sam Lai Yan Ting Liu Wen Jung Li Ya Jing Shen Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition Micromachines three-dimensional (3D) hydrogel assembly TiOPc alginate hydrogel light-induced electrodeposition |
| title | Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition |
| title_full | Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition |
| title_fullStr | Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition |
| title_full_unstemmed | Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition |
| title_short | Three-Dimensional Calcium Alginate Hydrogel Assembly via TiOPc-Based Light-Induced Controllable Electrodeposition |
| title_sort | three dimensional calcium alginate hydrogel assembly via tiopc based light induced controllable electrodeposition |
| topic | three-dimensional (3D) hydrogel assembly TiOPc alginate hydrogel light-induced electrodeposition |
| url | http://www.mdpi.com/2072-666X/8/6/192 |
| work_keys_str_mv | AT yangliu threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition AT congwu threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition AT hoksumsamlai threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition AT yantingliu threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition AT wenjungli threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition AT yajingshen threedimensionalcalciumalginatehydrogelassemblyviatiopcbasedlightinducedcontrollableelectrodeposition |