Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice
Anti-rheumatoid arthritis (RA) effects of α-tocopherol (α-T) have been shown in human patients in a double-blind trial. However, the effects of α-T and its derivatives on fibroblast-like synoviocytes (FLS) during the pathogenesis of RA remain unclear. In the present study, we compared the expression...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1425 |
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| author | Susumu Hama Naoko Kirimura Aki Obara Hirokatsu Takatsu Kentaro Kogure |
| author_facet | Susumu Hama Naoko Kirimura Aki Obara Hirokatsu Takatsu Kentaro Kogure |
| author_sort | Susumu Hama |
| collection | DOAJ |
| description | Anti-rheumatoid arthritis (RA) effects of α-tocopherol (α-T) have been shown in human patients in a double-blind trial. However, the effects of α-T and its derivatives on fibroblast-like synoviocytes (FLS) during the pathogenesis of RA remain unclear. In the present study, we compared the expression levels of genes related to RA progression in FLS treated with α-T, succinic ester of α-T (TS), and phosphate ester of α-T (TP), as determined via RT-PCR. The mRNA levels of interleukin (IL)-6, tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP)-3, and MMP-13 were reduced by treatment with TP without cytotoxicity, while α-T and TS did not show such effects. Furthermore, intraperitoneal injection of TP ameliorated the edema of the foot and joint and improved the arthritis score in laminarin-induced RA model mice. Therefore, TP exerted anti-RA effects through by inhibiting RA-related gene expression. |
| first_indexed | 2024-03-09T21:19:52Z |
| format | Article |
| id | doaj.art-84775a1e8926413fbbb4351ff535757d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:19:52Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-84775a1e8926413fbbb4351ff535757d2023-11-23T21:23:48ZengMDPI AGMolecules1420-30492022-02-01274142510.3390/molecules27041425Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in MiceSusumu Hama0Naoko Kirimura1Aki Obara2Hirokatsu Takatsu3Kentaro Kogure4Faculty of Pharmacy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, JapanDepartment of Biophysical Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, JapanDepartment of Analytical Chemistry, Kyorin University, Tokyo 181-8612, JapanDepartment of Analytical Chemistry, Kyorin University, Tokyo 181-8612, JapanGraduate School of Biomedical Sciences, Tokushima University, Tokushima 770-8505, JapanAnti-rheumatoid arthritis (RA) effects of α-tocopherol (α-T) have been shown in human patients in a double-blind trial. However, the effects of α-T and its derivatives on fibroblast-like synoviocytes (FLS) during the pathogenesis of RA remain unclear. In the present study, we compared the expression levels of genes related to RA progression in FLS treated with α-T, succinic ester of α-T (TS), and phosphate ester of α-T (TP), as determined via RT-PCR. The mRNA levels of interleukin (IL)-6, tumor necrosis factor-α (TNF-α), matrix metalloproteinase (MMP)-3, and MMP-13 were reduced by treatment with TP without cytotoxicity, while α-T and TS did not show such effects. Furthermore, intraperitoneal injection of TP ameliorated the edema of the foot and joint and improved the arthritis score in laminarin-induced RA model mice. Therefore, TP exerted anti-RA effects through by inhibiting RA-related gene expression.https://www.mdpi.com/1420-3049/27/4/1425α-tocopheroltocopheryl phosphatetocopheryl succinaterheumatoid arthritis |
| spellingShingle | Susumu Hama Naoko Kirimura Aki Obara Hirokatsu Takatsu Kentaro Kogure Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice Molecules α-tocopherol tocopheryl phosphate tocopheryl succinate rheumatoid arthritis |
| title | Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice |
| title_full | Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice |
| title_fullStr | Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice |
| title_full_unstemmed | Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice |
| title_short | Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice |
| title_sort | tocopheryl phosphate inhibits rheumatoid arthritis related gene expression in vitro and ameliorates arthritic symptoms in mice |
| topic | α-tocopherol tocopheryl phosphate tocopheryl succinate rheumatoid arthritis |
| url | https://www.mdpi.com/1420-3049/27/4/1425 |
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