Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis
Abstract Background Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory fea...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-07-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-021-06418-8 |
| _version_ | 1819151751331184640 |
|---|---|
| author | Sho Ogata Ken Shimizu Susumu Tominaga Susumu Matsukuma |
| author_facet | Sho Ogata Ken Shimizu Susumu Tominaga Susumu Matsukuma |
| author_sort | Sho Ogata |
| collection | DOAJ |
| description | Abstract Background Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically. Methods From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (male:female = 10:4; 28–73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed. Results iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05). Conclusions HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS. |
| first_indexed | 2024-12-22T14:38:22Z |
| format | Article |
| id | doaj.art-8478123a933d4283937b572e04044de2 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-22T14:38:22Z |
| publishDate | 2021-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-8478123a933d4283937b572e04044de22022-12-21T18:22:37ZengBMCBMC Infectious Diseases1471-23342021-07-012111710.1186/s12879-021-06418-8Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosisSho Ogata0Ken Shimizu1Susumu Tominaga2Susumu Matsukuma3Department of Pathology and Laboratory Medicine, National Defense Medical CollegeDepartment of Diagnostic Pathology, JCHO Saitama Medical CenterDepartment of Pathology and Laboratory Medicine, National Defense Medical CollegeDepartment of Pathology and Laboratory Medicine, National Defense Medical CollegeAbstract Background Human intestinal spirochetosis (HIS) is an infectious disease of large intestines caused by Brachyspira species, and most HIS cases are asymptomatic or exhibit mild intestinal symptoms. The host reaction to HIS remains unclear, and we examined HIS-related mucosal inflammatory features histologically. Methods From the archival HIS cases in a single medical center, 24 endoscopically taken specimens from 14 HIS cases (male:female = 10:4; 28–73 yrs) were selected as not containing polypoid or neoplastic lesions. Stromal neutrophils, eosinophils, and mast cells, and intraepithelial neutrophils and eosinophils, (sNeu, sEo, sMast, iNeu, and iEo, respectively) were counted, and the presence or absence of lymphoid follicles/aggregates (LFs) was also examined. Association of the above inflammation parameters and spirochetal infection parameters (such as degrees of characteristic fringe distribution, of spirochetal cryptal invasion, and of spirochetal intraepithelial invasion) were also analysed. Results iNeu was observed in 29.2%, iEo in 58.3%, and LFs in 50.0% of the specimens. Maximal counts of sNeu, sEo, sMast, iNeu, and iEo averaged 8.4, 21.5, 6.0, 0.5 and 1.5, respectively. Strong correlation between the maximum counts of iNeu and iEo (p < 0.001, r = 0.81), and correlations between those of iEo and sNeu (p = 0.0012, r = 0.62) and between those of iEo and sEo (p = 0.026, r = 0.45) were observed. iNeu was influenced by fringe formation (p < 0.05) and spirochetal crypt involvement (p < 0.05). Conclusions HIS was accompanied by inflammatory reactions, and among these, mucosal eosinophilic infiltration may be a central indicator and host reaction of HIS.https://doi.org/10.1186/s12879-021-06418-8BrachyspiraEosinophilHuman intestinal spirochetosisSpirochete |
| spellingShingle | Sho Ogata Ken Shimizu Susumu Tominaga Susumu Matsukuma Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis BMC Infectious Diseases Brachyspira Eosinophil Human intestinal spirochetosis Spirochete |
| title | Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| title_full | Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| title_fullStr | Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| title_full_unstemmed | Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| title_short | Mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| title_sort | mucosal eosinophilic infiltration may be a characteristic of human intestinal spirochetosis |
| topic | Brachyspira Eosinophil Human intestinal spirochetosis Spirochete |
| url | https://doi.org/10.1186/s12879-021-06418-8 |
| work_keys_str_mv | AT shoogata mucosaleosinophilicinfiltrationmaybeacharacteristicofhumanintestinalspirochetosis AT kenshimizu mucosaleosinophilicinfiltrationmaybeacharacteristicofhumanintestinalspirochetosis AT susumutominaga mucosaleosinophilicinfiltrationmaybeacharacteristicofhumanintestinalspirochetosis AT susumumatsukuma mucosaleosinophilicinfiltrationmaybeacharacteristicofhumanintestinalspirochetosis |