Simultaneous absolute protein quantification of seven cytochrome P450 isoforms in rat liver microsomes by LC-MS/MS-based isotope internal standard method

Simultaneous absolute protein quantification of seven cytochrome P450 isoforms in rat liver microsomes by LC-MS/MS-based isotope internal standard method

The cytochrome P450 (CYP) enzymes play a pivotal role in drug metabolism. LC-MS/MS-based targeting technology has been applied to the analysis of CYP enzymes, promoting drug development and drug-drug interaction studies. Rat is one of the most commonly used models for drug metabolism assessment, but...

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Bibliographic Details
Main Authors: Fulin Jiang, Chang Zhang, Zihan Lu, Jingyu Liu, Peiqing Liu, Min Huang, Guoping Zhong
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-08-01
Series:Frontiers in Pharmacology
Subjects:
cytochrome P450
absolute quantification
LC-MS/MS
rat liver microsomes
isotope internal standard
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2022.906027/full

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