Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province
Psammosilene tunicoides is an important ethnic medicine in Southwest China and the main raw material for Chinese patent medicines like Yunnan Baiyao. The purpose of this study was to isolate and identify the pathogenic fungus of root rot of Psammosilene tunicoides in Yunnan. In this study, the disea...
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| Format: | Article |
| Language: | zho |
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China Science Publishing & Media Ltd. (CSPM)
2023-01-01
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| Series: | Guangxi Zhiwu |
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| Online Access: | https://doi.org/10.11931/guihaia.gxzw202205027 |
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| author | Wenting YANG Xiaomei ZHANG Guodong LI Junnan LI Chengfan YANG Aili ZHANG |
| author_facet | Wenting YANG Xiaomei ZHANG Guodong LI Junnan LI Chengfan YANG Aili ZHANG |
| author_sort | Wenting YANG |
| collection | DOAJ |
| description | Psammosilene tunicoides is an important ethnic medicine in Southwest China and the main raw material for Chinese patent medicines like Yunnan Baiyao. The purpose of this study was to isolate and identify the pathogenic fungus of root rot of Psammosilene tunicoides in Yunnan. In this study, the disease-health junction of the root tissue of diseased plant was isolated and purified by pure culture method to obtain the main pathogenic microorganisms of P. tunicoides. Pathogenicity of isolated pathogenic microorganisms was analyzed the according to Koch’s Postulates, and then were identified by morphological observation, rDNA-ITS and TEF-1α sequence analysis and phylogenetic tree construction. The results were as follows: (1) A total of 85 strains of fungi were obtained, among which PSD-1, PSD-2 and PSD-3 of Fusarium fungi caused root rot of Psammosilene tunicoides. (2) These three strains were re-inoculated into the P. tunicoides, which produced similar disease symptoms occurred with field plants, and the disease incidences after inoculation with pathogenic fungi were 60%, 61.7% and 71.7%, respectively. (3) Combined with morphological observation and gene sequence analysis, the three strains were identified as Fusarium oxysporum. This study firstly found that the F. oxysporum was one of the main pathogenic fungi causing root rot of Psammosilene tunicoides. The results of this study lay a foundation for the in-depth exploration of the endophytic flora with biological control of root rot and its mechanism, and serve as a theoretical basis for the follow-up prevention and control of the disease. |
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| last_indexed | 2024-03-07T20:02:13Z |
| publishDate | 2023-01-01 |
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| series | Guangxi Zhiwu |
| spelling | doaj.art-8480797402b74a749f552cc74bc6d1b42024-02-28T05:57:30ZzhoChina Science Publishing & Media Ltd. (CSPM)Guangxi Zhiwu1000-31422023-01-01431798710.11931/guihaia.gxzw2022050271000-3142(2023)01-0079-09Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan ProvinceWenting YANG0Xiaomei ZHANG1Guodong LI2Junnan LI3Chengfan YANG4Aili ZHANG5Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaYunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine/College of Traditional Chinese Medicine and Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan University of Chinese Medicine, Kunming 650500, ChinaPsammosilene tunicoides is an important ethnic medicine in Southwest China and the main raw material for Chinese patent medicines like Yunnan Baiyao. The purpose of this study was to isolate and identify the pathogenic fungus of root rot of Psammosilene tunicoides in Yunnan. In this study, the disease-health junction of the root tissue of diseased plant was isolated and purified by pure culture method to obtain the main pathogenic microorganisms of P. tunicoides. Pathogenicity of isolated pathogenic microorganisms was analyzed the according to Koch’s Postulates, and then were identified by morphological observation, rDNA-ITS and TEF-1α sequence analysis and phylogenetic tree construction. The results were as follows: (1) A total of 85 strains of fungi were obtained, among which PSD-1, PSD-2 and PSD-3 of Fusarium fungi caused root rot of Psammosilene tunicoides. (2) These three strains were re-inoculated into the P. tunicoides, which produced similar disease symptoms occurred with field plants, and the disease incidences after inoculation with pathogenic fungi were 60%, 61.7% and 71.7%, respectively. (3) Combined with morphological observation and gene sequence analysis, the three strains were identified as Fusarium oxysporum. This study firstly found that the F. oxysporum was one of the main pathogenic fungi causing root rot of Psammosilene tunicoides. The results of this study lay a foundation for the in-depth exploration of the endophytic flora with biological control of root rot and its mechanism, and serve as a theoretical basis for the follow-up prevention and control of the disease.https://doi.org/10.11931/guihaia.gxzw202205027psammosilene tunicoidesroot rotisolationidentificationfusarium oxysporum |
| spellingShingle | Wenting YANG Xiaomei ZHANG Guodong LI Junnan LI Chengfan YANG Aili ZHANG Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province Guangxi Zhiwu psammosilene tunicoides root rot isolation identification fusarium oxysporum |
| title | Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province |
| title_full | Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province |
| title_fullStr | Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province |
| title_full_unstemmed | Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province |
| title_short | Isolation and identification of pathogens causing root rot disease of Psammosilene tunicoides in Yunnan Province |
| title_sort | isolation and identification of pathogens causing root rot disease of psammosilene tunicoides in yunnan province |
| topic | psammosilene tunicoides root rot isolation identification fusarium oxysporum |
| url | https://doi.org/10.11931/guihaia.gxzw202205027 |
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