Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway
Endothelial inflammation is a multifaceted physiological process that plays a pivotal role in the pathogenesis and progression of diverse diseases, encompassing but not limited to acute lung infections like COVID-19, coronary artery disease, stroke, sepsis, metabolic syndrome, certain malignancies,...
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/6/1239 |
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| author | Li Liu Liang Qiu Jing Xue Chao Zhong Manman Qin Yifeng Zhang Chuanming Xu Yanfei Xie Jun Yu |
| author_facet | Li Liu Liang Qiu Jing Xue Chao Zhong Manman Qin Yifeng Zhang Chuanming Xu Yanfei Xie Jun Yu |
| author_sort | Li Liu |
| collection | DOAJ |
| description | Endothelial inflammation is a multifaceted physiological process that plays a pivotal role in the pathogenesis and progression of diverse diseases, encompassing but not limited to acute lung infections like COVID-19, coronary artery disease, stroke, sepsis, metabolic syndrome, certain malignancies, and even psychiatric disorders such as depression. This inflammatory response is characterized by augmented expression of adhesion molecules and secretion of pro-inflammatory cytokines. In this study, we discovered that saponins from <i>Allium macrostemon bulbs</i> (SAMB) effectively inhibited inflammation in human umbilical vein endothelial cells induced by the exogenous inflammatory mediator lipopolysaccharide or the endogenous inflammatory mediator tumor necrosis factor-α, as evidenced by a significant reduction in the expression of pro-inflammatory factors and vascular cell adhesion molecule-1 (VCAM-1) with decreased monocyte adhesion. By employing the NF-κB inhibitor BAY-117082, we demonstrated that the inhibitory effect of SAMB on VCAM-1 expression may be attributed to the NF-κB pathway’s inactivation, as characterized by the suppressed IκBα degradation and NF-κB p65 phosphorylation. Subsequently, we employed a murine model of lipopolysaccharide-induced septic acute lung injury to substantiate the potential of SAMB in ameliorating endothelial inflammation and acute lung injury in vivo. These findings provide novel insight into potential preventive and therapeutic strategies for the clinical management of diseases associated with endothelial inflammation. |
| first_indexed | 2024-04-24T17:58:24Z |
| format | Article |
| id | doaj.art-84824ea01d7040b891d59ff2ef758c3d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-24T17:58:24Z |
| publishDate | 2024-03-01 |
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| series | Molecules |
| spelling | doaj.art-84824ea01d7040b891d59ff2ef758c3d2024-03-27T13:56:49ZengMDPI AGMolecules1420-30492024-03-01296123910.3390/molecules29061239Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 PathwayLi Liu0Liang Qiu1Jing Xue2Chao Zhong3Manman Qin4Yifeng Zhang5Chuanming Xu6Yanfei Xie7Jun Yu8Center for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaCenter for Translational Medicine, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaDepartment of Cardiovascular Sciences and Center for Metabolic Disease Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USAEndothelial inflammation is a multifaceted physiological process that plays a pivotal role in the pathogenesis and progression of diverse diseases, encompassing but not limited to acute lung infections like COVID-19, coronary artery disease, stroke, sepsis, metabolic syndrome, certain malignancies, and even psychiatric disorders such as depression. This inflammatory response is characterized by augmented expression of adhesion molecules and secretion of pro-inflammatory cytokines. In this study, we discovered that saponins from <i>Allium macrostemon bulbs</i> (SAMB) effectively inhibited inflammation in human umbilical vein endothelial cells induced by the exogenous inflammatory mediator lipopolysaccharide or the endogenous inflammatory mediator tumor necrosis factor-α, as evidenced by a significant reduction in the expression of pro-inflammatory factors and vascular cell adhesion molecule-1 (VCAM-1) with decreased monocyte adhesion. By employing the NF-κB inhibitor BAY-117082, we demonstrated that the inhibitory effect of SAMB on VCAM-1 expression may be attributed to the NF-κB pathway’s inactivation, as characterized by the suppressed IκBα degradation and NF-κB p65 phosphorylation. Subsequently, we employed a murine model of lipopolysaccharide-induced septic acute lung injury to substantiate the potential of SAMB in ameliorating endothelial inflammation and acute lung injury in vivo. These findings provide novel insight into potential preventive and therapeutic strategies for the clinical management of diseases associated with endothelial inflammation.https://www.mdpi.com/1420-3049/29/6/1239saponins from <i>Allium macrostemon bulbs</i>endothelial inflammationVCAM-1acute lung injury |
| spellingShingle | Li Liu Liang Qiu Jing Xue Chao Zhong Manman Qin Yifeng Zhang Chuanming Xu Yanfei Xie Jun Yu Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway Molecules saponins from <i>Allium macrostemon bulbs</i> endothelial inflammation VCAM-1 acute lung injury |
| title | Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway |
| title_full | Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway |
| title_fullStr | Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway |
| title_full_unstemmed | Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway |
| title_short | Saponins from <i>Allium macrostemon Bulbs</i> Attenuate Endothelial Inflammation and Acute Lung Injury via the NF-κB/VCAM-1 Pathway |
| title_sort | saponins from i allium macrostemon bulbs i attenuate endothelial inflammation and acute lung injury via the nf κb vcam 1 pathway |
| topic | saponins from <i>Allium macrostemon bulbs</i> endothelial inflammation VCAM-1 acute lung injury |
| url | https://www.mdpi.com/1420-3049/29/6/1239 |
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