An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran
Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of...
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004&lng=en&tlng=en |
| _version_ | 1818040938079977472 |
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| author | Mahin Farahmand Hossein Nahrevanian Hasti Atashi Shirazi Sabah Naeimi Zahra Farzanehnejad |
| author_facet | Mahin Farahmand Hossein Nahrevanian Hasti Atashi Shirazi Sabah Naeimi Zahra Farzanehnejad |
| author_sort | Mahin Farahmand |
| collection | DOAJ |
| description | Cutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed and samples were examined for CL by using both direct microscopic and culture methods. Moreover, PCR assay was applied for detection of Leishmania species in CL isolates resulting from parasitological assay. Clinical observation revealed that the majority (58%) of lesions was single; double lesions were observed in 22% of patients, and only 20% of CL had multiple lesions. Out of 200 patients, Leishman body was observed in 77 samples (38.5%) by direct smear and 40% by cultivation assay. Most patients (21.3%) had a travel history to the Isfahan province, one of the most important endemic areas of CL located in center of Iran. PCR assay by kDNA indicated 32 and 18 out of 50 isolates respectively had similar patterns with standard L. major and L. tropica. In conclusion, clinical manifestations and an appropriate diagnostic assay with a parallel molecular characterization of CL may lead to a screening evaluation of disease, prognosis, treatment and control strategies. |
| first_indexed | 2024-12-10T08:22:28Z |
| format | Article |
| id | doaj.art-8486fbb21ef34c19935e43f703060700 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-10T08:22:28Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-8486fbb21ef34c19935e43f7030607002022-12-22T01:56:19ZengElsevierBrazilian Journal of Infectious Diseases1678-4391151172110.1590/S1413-86702011000100004S1413-86702011000100004An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in IranMahin Farahmand0Hossein Nahrevanian1Hasti Atashi Shirazi2Sabah Naeimi3Zahra Farzanehnejad4Pasteur InstitutePasteur InstitutePasteur InstitutePasteur InstitutePasteur InstituteCutaneous leishmaniasis (CL) is a widespread tropical infection which has a high incidence rate in Iran. Leishmania tropica, the causative agent of anthroponotic cutaneous leishmaniasis (ACL), and Leishmania major, which causes zoonotic cutaneous leishmaniasis (ZCL), are endemic in various parts of Iran with a high incidence rate. The aim of this study was to evaluate the reappraisal of the diagnosis and epidemiology of CL in Iran, by different clinical, parasitological and molecular assays among patients suspected of CL referred to the Department of Parasitology, at the Pasteur Institute of Iran during 2006-2009. Two hundred samples from patients with ulcerative skin lesions were collected, clinical analyses were applied, data questionnaire was completed and samples were examined for CL by using both direct microscopic and culture methods. Moreover, PCR assay was applied for detection of Leishmania species in CL isolates resulting from parasitological assay. Clinical observation revealed that the majority (58%) of lesions was single; double lesions were observed in 22% of patients, and only 20% of CL had multiple lesions. Out of 200 patients, Leishman body was observed in 77 samples (38.5%) by direct smear and 40% by cultivation assay. Most patients (21.3%) had a travel history to the Isfahan province, one of the most important endemic areas of CL located in center of Iran. PCR assay by kDNA indicated 32 and 18 out of 50 isolates respectively had similar patterns with standard L. major and L. tropica. In conclusion, clinical manifestations and an appropriate diagnostic assay with a parallel molecular characterization of CL may lead to a screening evaluation of disease, prognosis, treatment and control strategies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004&lng=en&tlng=enparasitological analysismolecular biologyleishmaniasiscutaneousIranepidemiology |
| spellingShingle | Mahin Farahmand Hossein Nahrevanian Hasti Atashi Shirazi Sabah Naeimi Zahra Farzanehnejad An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran Brazilian Journal of Infectious Diseases parasitological analysis molecular biology leishmaniasis cutaneous Iran epidemiology |
| title | An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran |
| title_full | An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran |
| title_fullStr | An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran |
| title_full_unstemmed | An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran |
| title_short | An overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in Iran |
| title_sort | overview of a diagnostic and epidemiologic reappraisal of cutaneous leishmaniasis in iran |
| topic | parasitological analysis molecular biology leishmaniasis cutaneous Iran epidemiology |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100004&lng=en&tlng=en |
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