Differential expression of transposable elements in the medaka melanoma model.
Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0251713 |
| _version_ | 1818342769482006528 |
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| author | Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl |
| author_facet | Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl |
| author_sort | Frederik Helmprobst |
| collection | DOAJ |
| description | Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish. |
| first_indexed | 2024-12-13T16:19:57Z |
| format | Article |
| id | doaj.art-84879b231800422dad66f0d25963e9aa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T16:19:57Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-84879b231800422dad66f0d25963e9aa2022-12-21T23:38:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610e025171310.1371/journal.pone.0251713Differential expression of transposable elements in the medaka melanoma model.Frederik HelmprobstSusanne KneitzBarbara KlotzMagali NavilleCorentin DechaudJean-Nicolas VolffManfred SchartlMalignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.https://doi.org/10.1371/journal.pone.0251713 |
| spellingShingle | Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl Differential expression of transposable elements in the medaka melanoma model. PLoS ONE |
| title | Differential expression of transposable elements in the medaka melanoma model. |
| title_full | Differential expression of transposable elements in the medaka melanoma model. |
| title_fullStr | Differential expression of transposable elements in the medaka melanoma model. |
| title_full_unstemmed | Differential expression of transposable elements in the medaka melanoma model. |
| title_short | Differential expression of transposable elements in the medaka melanoma model. |
| title_sort | differential expression of transposable elements in the medaka melanoma model |
| url | https://doi.org/10.1371/journal.pone.0251713 |
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