Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation
Molecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles...
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MDPI AG
2022-12-01
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| author | Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova |
| author_facet | Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova |
| author_sort | Yulia L. Moshchenskaya |
| collection | DOAJ |
| description | Molecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles of serine and cysteine proteases (endopeptidases), endonucleases, and metacaspases families are often considered markers of the final xylogenesis stage. In the present study, we examined the gene expression profiles of the <i>BFN</i> (bifunctional endonuclease) family—<i>BFN</i>, <i>BFN1</i>, <i>BFN2</i>, <i>BFN3</i>, and peptidase (cysteine endopeptidase, <i>CEP</i> and metacaspase, <i>MC5</i>) in the radial row, in addition to the vascular phloem and cambium (F1), differentiating xylem (F2), sapwood (SW), and transition zone during the active cambial growth period of uneven-aged pine trees (25-, 63- and 164-cambial age (c.a.) years old). We have shown that the expression patterns of the PCD-related genes did not depend on the cambial age but were largely determined by plant tissue type. In the radial row F1-F2-SW, we studied the activities of enzymes, including sucrose in metabolism (sucrose synthase, three forms of invertase); antioxidant system (AOS) enzymes (superoxide dismutase, catalase); and peroxidase andpolyphenol oxidase, which belonged to AOS enzymes and were involved in the synthesis of phenolic components of cell walls. The activity of the enzymes indicated that the trunk tissues of pine trees had varying metabolic status. Molecular genetic PCD regulation mechanisms during xylem vascular and mechanical element formation and parenchyma cells’ PCD during the formation of Scots pine heartwood were discussed. |
| first_indexed | 2024-03-09T15:57:32Z |
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| language | English |
| last_indexed | 2024-03-09T15:57:32Z |
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| spelling | doaj.art-848a96211c6e4617861badc78c94b7642023-11-24T17:27:17ZengMDPI AGPlants2223-77472022-12-011124343810.3390/plants11243438Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue FormationYulia L. Moshchenskaya0Natalia A. Galibina1Kseniya M. Nikerova2Tatiana V. Tarelkina3Maksim A. Korzhenevsky4Irina N. Sofronova5Maria A. Ershova6Ludmila I. Semenova7Forest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaForest Research Institute, Karelian Research Centre of the Russian Academy of Sciences, 11 Pushkinskaya st., 185910 Petrozavodsk, RussiaMolecular genetic markers of various PCD (programmed cell death) variants during xylo- and phloemogenesis have been identified for the first time in Scots pine under lingonberry pine forest conditions in Northwest Russia (middle taiga subzone). PCD is a genetically determined process. Gene profiles of serine and cysteine proteases (endopeptidases), endonucleases, and metacaspases families are often considered markers of the final xylogenesis stage. In the present study, we examined the gene expression profiles of the <i>BFN</i> (bifunctional endonuclease) family—<i>BFN</i>, <i>BFN1</i>, <i>BFN2</i>, <i>BFN3</i>, and peptidase (cysteine endopeptidase, <i>CEP</i> and metacaspase, <i>MC5</i>) in the radial row, in addition to the vascular phloem and cambium (F1), differentiating xylem (F2), sapwood (SW), and transition zone during the active cambial growth period of uneven-aged pine trees (25-, 63- and 164-cambial age (c.a.) years old). We have shown that the expression patterns of the PCD-related genes did not depend on the cambial age but were largely determined by plant tissue type. In the radial row F1-F2-SW, we studied the activities of enzymes, including sucrose in metabolism (sucrose synthase, three forms of invertase); antioxidant system (AOS) enzymes (superoxide dismutase, catalase); and peroxidase andpolyphenol oxidase, which belonged to AOS enzymes and were involved in the synthesis of phenolic components of cell walls. The activity of the enzymes indicated that the trunk tissues of pine trees had varying metabolic status. Molecular genetic PCD regulation mechanisms during xylem vascular and mechanical element formation and parenchyma cells’ PCD during the formation of Scots pine heartwood were discussed.https://www.mdpi.com/2223-7747/11/24/3438<i>Pinus sylvestris</i> L.programmed cell deathxylogenesisheartwood formationbifunctional endonucleasecysteine endopeptidase |
| spellingShingle | Yulia L. Moshchenskaya Natalia A. Galibina Kseniya M. Nikerova Tatiana V. Tarelkina Maksim A. Korzhenevsky Irina N. Sofronova Maria A. Ershova Ludmila I. Semenova Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation Plants <i>Pinus sylvestris</i> L. programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase |
| title | Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation |
| title_full | Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation |
| title_fullStr | Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation |
| title_full_unstemmed | Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation |
| title_short | Plant-Programmed Cell Death-Associated Genes Participation in <i>Pinus sylvestris</i> L. Trunk Tissue Formation |
| title_sort | plant programmed cell death associated genes participation in i pinus sylvestris i l trunk tissue formation |
| topic | <i>Pinus sylvestris</i> L. programmed cell death xylogenesis heartwood formation bifunctional endonuclease cysteine endopeptidase |
| url | https://www.mdpi.com/2223-7747/11/24/3438 |
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