An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

ABSTRACT DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia coli BW3KD strain with higher transf...

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Bibliographic Details
Main Authors: Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Format: Article
Language:English
Published: American Society for Microbiology 2022-12-01
Series:Microbiology Spectrum
Subjects:
DNA assembly
Escherichia coli
chemical competent cells
cloning
transformation
Online Access:https://journals.asm.org/doi/10.1128/spectrum.02497-22

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