Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst
Abstract Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (CuxO/TiO2) inactivated the severe acute respiratory syndrome c...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-09402-7 |
| _version_ | 1828846874226851840 |
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| author | Ryuichi Nakano Akira Yamaguchi Kayano Sunada Takeshi Nagai Akiyo Nakano Yuki Suzuki Hisakazu Yano Hitoshi Ishiguro Masahiro Miyauchi |
| author_facet | Ryuichi Nakano Akira Yamaguchi Kayano Sunada Takeshi Nagai Akiyo Nakano Yuki Suzuki Hisakazu Yano Hitoshi Ishiguro Masahiro Miyauchi |
| author_sort | Ryuichi Nakano |
| collection | DOAJ |
| description | Abstract Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (CuxO/TiO2) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the CuxO/TiO2 photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the CuxO/TiO2 photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The CuxO/TiO2 photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night. |
| first_indexed | 2024-12-12T21:58:55Z |
| format | Article |
| id | doaj.art-848c3580c975495b985e7b314ded659f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T21:58:55Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-848c3580c975495b985e7b314ded659f2022-12-22T00:10:35ZengNature PortfolioScientific Reports2045-23222022-04-0112111010.1038/s41598-022-09402-7Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalystRyuichi Nakano0Akira Yamaguchi1Kayano Sunada2Takeshi Nagai3Akiyo Nakano4Yuki Suzuki5Hisakazu Yano6Hitoshi Ishiguro7Masahiro Miyauchi8Department of Microbiology and Infectious Diseases, Nara Medical UniversityDepartment of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of TechnologyKanagawa Institute of Industrial Science and Technology (KISTEC)Kanagawa Institute of Industrial Science and Technology (KISTEC)Department of Microbiology and Infectious Diseases, Nara Medical UniversityDepartment of Microbiology and Infectious Diseases, Nara Medical UniversityDepartment of Microbiology and Infectious Diseases, Nara Medical UniversityKanagawa Institute of Industrial Science and Technology (KISTEC)Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of TechnologyAbstract Photocatalysts are promising materials for solid-state antiviral coatings to protect against the spread of pandemic coronavirus disease (COVID-19). This paper reports that copper oxide nanoclusters grafted with titanium dioxide (CuxO/TiO2) inactivated the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, including its Delta variant, even under dark condition, and further inactivated it under illumination with a white fluorescent bulb. To investigate its inactivation mechanism, the denaturation of spike proteins of SARS-CoV-2 was examined by sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) and enzyme-linked immunosorbent assay (ELISA). In addition to spike proteins, fragmentation of ribonucleic acids in SARS-CoV-2 was investigated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). As a result, both spike proteins and RNAs in the SARS-CoV-2 virus were damaged by the CuxO/TiO2 photocatalyst even under dark condition and were further damaged under white fluorescent bulb illumination. Based on the present antiviral mechanism, the CuxO/TiO2 photocatalyst will be effective in inactivating other potential mutant strains of SARS-CoV-2. The CuxO/TiO2 photocatalyst can thus be used to reduce the infectious risk of COVID-19 in an indoor environment, where light illumination is turned on during the day and off during the night.https://doi.org/10.1038/s41598-022-09402-7 |
| spellingShingle | Ryuichi Nakano Akira Yamaguchi Kayano Sunada Takeshi Nagai Akiyo Nakano Yuki Suzuki Hisakazu Yano Hitoshi Ishiguro Masahiro Miyauchi Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst Scientific Reports |
| title | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst |
| title_full | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst |
| title_fullStr | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst |
| title_full_unstemmed | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst |
| title_short | Inactivation of various variant types of SARS-CoV-2 by indoor-light-sensitive TiO2-based photocatalyst |
| title_sort | inactivation of various variant types of sars cov 2 by indoor light sensitive tio2 based photocatalyst |
| url | https://doi.org/10.1038/s41598-022-09402-7 |
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