Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre
Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The...
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| Format: | Article |
| Language: | English |
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Light House Polyclinic Mangalore
2019-04-01
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| Series: | Online Journal of Health & Allied Sciences |
| Subjects: | |
| Online Access: | https://www.ojhas.org/issue69/2019-1-7.html |
| _version_ | 1818642054095306752 |
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| author | Mohandoss Murugesan Chandran K Nair Sangeetha K Nayanar Kalyana Chakravarthy Pentapati |
| author_facet | Mohandoss Murugesan Chandran K Nair Sangeetha K Nayanar Kalyana Chakravarthy Pentapati |
| author_sort | Mohandoss Murugesan |
| collection | DOAJ |
| description | Background: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference between the duplicate samples with respect to low (CD34+ cells/µl<500; P=0.632), Medium: 500 to 1000 CD34+ cells/µl, P=0.085 and High >1000 CD34+ cells/µl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations. |
| first_indexed | 2024-12-16T23:36:57Z |
| format | Article |
| id | doaj.art-848efb73107145b68905e883901b4fb7 |
| institution | Directory Open Access Journal |
| issn | 0972-5997 0972-5997 |
| language | English |
| last_indexed | 2024-12-16T23:36:57Z |
| publishDate | 2019-04-01 |
| publisher | Light House Polyclinic Mangalore |
| record_format | Article |
| series | Online Journal of Health & Allied Sciences |
| spelling | doaj.art-848efb73107145b68905e883901b4fb72022-12-21T22:11:44ZengLight House Polyclinic MangaloreOnline Journal of Health & Allied Sciences0972-59970972-59972019-04-01181Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer CentreMohandoss Murugesan0Chandran K Nair1Sangeetha K Nayanar2Kalyana Chakravarthy Pentapati3Department of Transfusion Medicine, Malabar Cancer Centre, Thalassery, KeralaDepartment of Clinical Hematology, Malabar Cancer Centre, Thalassery, KeralaDepartment of Oncopathology, Malabar Cancer Centre, Thalassery, KeralaDepartment of Public Health Dentistry, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, KarnatakaBackground: The success of peripheral blood stem cell (PBSC) transplant often relies on CD34+ cells counting. As the assay needs to be repeated in duplicate, this study was conducted to observe the variations between the duplicates with respect to concentration of leukapheresis product. Methods: The assay followed was single platform method - ISHAGE protocol published by the International Society of Haematotherapy and Graft Engineering. New parameter named variation index was used to compare the difference between the duplicates. Results: We present our data on 111 samples collected from leukapheresis product. 10% of the assay did not meet the criteria and required repeat testing. We observed no significant difference between the duplicate samples with respect to low (CD34+ cells/µl<500; P=0.632), Medium: 500 to 1000 CD34+ cells/µl, P=0.085 and High >1000 CD34+ cells/µl, P=0.864). Conclusion: Testing the CD34 enumeration in duplicate and verifying its repeatability for variation index of less than 10 will help in accurately determining CD34+ dose calculations.https://www.ojhas.org/issue69/2019-1-7.htmlCD34+ cells EnumerationFlowcytometryISHAGE |
| spellingShingle | Mohandoss Murugesan Chandran K Nair Sangeetha K Nayanar Kalyana Chakravarthy Pentapati Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre Online Journal of Health & Allied Sciences CD34+ cells Enumeration Flowcytometry ISHAGE |
| title | Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre |
| title_full | Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre |
| title_fullStr | Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre |
| title_full_unstemmed | Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre |
| title_short | Analysis of Flow Cytometric Enumeration of CD34+ cells in Leukapheresis Product from South Indian Cancer Centre |
| title_sort | analysis of flow cytometric enumeration of cd34 cells in leukapheresis product from south indian cancer centre |
| topic | CD34+ cells Enumeration Flowcytometry ISHAGE |
| url | https://www.ojhas.org/issue69/2019-1-7.html |
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