An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn
We reported a highly efficient electrochemical immunosensor utilizing chitosan–graphene nanosheets (CS-GNs) nanocomposites for the detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in corn samples. The CS-GNs nanocomposites, serving as a modifying layer, provide a signif...
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MDPI AG
2024-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/7/1461 |
| _version_ | 1827286709728968704 |
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| author | Shuai Zhang Caizhang Wu Zhike Zhao Kun Xu |
| author_facet | Shuai Zhang Caizhang Wu Zhike Zhao Kun Xu |
| author_sort | Shuai Zhang |
| collection | DOAJ |
| description | We reported a highly efficient electrochemical immunosensor utilizing chitosan–graphene nanosheets (CS-GNs) nanocomposites for the detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in corn samples. The CS-GNs nanocomposites, serving as a modifying layer, provide a significant specific surface area and biocompatibility, thereby enhancing both the electron transfer rate and the efficiency of antibody immobilization. The electrochemical characterization was conducted utilizing both differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Moreover, the antibody concentration, pH, antibody immobilization time, and immunoreaction time, were optimized. The results showed that the current change (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>Δ</mo><mi>I</mi></mrow></semantics></math></inline-formula>) before and after the immunoreaction demonstrated a strong linear relationship (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mi>R</mi><mn>2</mn></msup><mo>=</mo><mn>0.990</mn></mrow></semantics></math></inline-formula>) with the AFB<sub>1</sub> concentration, as well as good specificity and stability. The linear range extended from 0.05 to 25 ng/mL, with a detection limit of 0.021 ng/mL (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>S</mi><mo>/</mo><mi>N</mi><mo>=</mo><mn>3</mn></mrow></semantics></math></inline-formula>). The immunosensor exhibited a recovery rate ranging from 97.3% to 101.4% in corn samples, showing a promising performance using an efficient method, and indicating a remarkable prospect for the detection of fungal toxins in grains. |
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| language | English |
| last_indexed | 2024-04-24T10:39:12Z |
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| series | Molecules |
| spelling | doaj.art-8491c440fde2449b9c8a9ddb4a1900742024-04-12T13:23:07ZengMDPI AGMolecules1420-30492024-03-01297146110.3390/molecules29071461An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in CornShuai Zhang0Caizhang Wu1Zhike Zhao2Kun Xu3Key Laboratory of Grain Information Processing and Control (Henan University of Technology), Ministry of Education, Zhengzhou 450001, ChinaCollege of Electrical Engineering, Henan University of Technology, Zhengzhou 450001, ChinaCollege of Electrical Engineering, Henan University of Technology, Zhengzhou 450001, ChinaKey Laboratory of Grain Information Processing and Control (Henan University of Technology), Ministry of Education, Zhengzhou 450001, ChinaWe reported a highly efficient electrochemical immunosensor utilizing chitosan–graphene nanosheets (CS-GNs) nanocomposites for the detection of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) in corn samples. The CS-GNs nanocomposites, serving as a modifying layer, provide a significant specific surface area and biocompatibility, thereby enhancing both the electron transfer rate and the efficiency of antibody immobilization. The electrochemical characterization was conducted utilizing both differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). Moreover, the antibody concentration, pH, antibody immobilization time, and immunoreaction time, were optimized. The results showed that the current change (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mo>Δ</mo><mi>I</mi></mrow></semantics></math></inline-formula>) before and after the immunoreaction demonstrated a strong linear relationship (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mi>R</mi><mn>2</mn></msup><mo>=</mo><mn>0.990</mn></mrow></semantics></math></inline-formula>) with the AFB<sub>1</sub> concentration, as well as good specificity and stability. The linear range extended from 0.05 to 25 ng/mL, with a detection limit of 0.021 ng/mL (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>S</mi><mo>/</mo><mi>N</mi><mo>=</mo><mn>3</mn></mrow></semantics></math></inline-formula>). The immunosensor exhibited a recovery rate ranging from 97.3% to 101.4% in corn samples, showing a promising performance using an efficient method, and indicating a remarkable prospect for the detection of fungal toxins in grains.https://www.mdpi.com/1420-3049/29/7/1461electrochemical immunosensoraflatoxin B<sub>1</sub>chitosangraphene nanosheetsnanocomposites |
| spellingShingle | Shuai Zhang Caizhang Wu Zhike Zhao Kun Xu An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn Molecules electrochemical immunosensor aflatoxin B<sub>1</sub> chitosan graphene nanosheets nanocomposites |
| title | An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn |
| title_full | An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn |
| title_fullStr | An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn |
| title_full_unstemmed | An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn |
| title_short | An Electrochemical Immunosensor Based on Chitosan–Graphene Nanosheets for Aflatoxin B1 Detection in Corn |
| title_sort | electrochemical immunosensor based on chitosan graphene nanosheets for aflatoxin b1 detection in corn |
| topic | electrochemical immunosensor aflatoxin B<sub>1</sub> chitosan graphene nanosheets nanocomposites |
| url | https://www.mdpi.com/1420-3049/29/7/1461 |
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