An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary
The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (<i>Platichthys flesus</i>) collected after the spawning season in the heavily polluted Seine estuar...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-10-01
|
| Series: | Journal of Xenobiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2039-4713/10/2/4 |
| _version_ | 1797549681999347712 |
|---|---|
| author | Elodie Borcier Grégory Charrier Jérôme Couteau Géraldine Maillet Fabienne Le Grand Antoine Bideau Matthieu Waeles Stéphane Le Floch Rachid Amara Vianney Pichereau Jean Laroche |
| author_facet | Elodie Borcier Grégory Charrier Jérôme Couteau Géraldine Maillet Fabienne Le Grand Antoine Bideau Matthieu Waeles Stéphane Le Floch Rachid Amara Vianney Pichereau Jean Laroche |
| author_sort | Elodie Borcier |
| collection | DOAJ |
| description | The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (<i>Platichthys flesus</i>) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between membrane phospholipids, xenobiotic metabolism, bioenergetics, and antioxidant defence. The genetic diversity considering neutral markers was maintained in the heavily polluted Seine population compared with the Douarnenez population. Finally, we suggest that the high physiological cost of tolerance to toxicants in the Seine flounder population could compromise its capacity to respond in the future to an additional stressor like warming waters in shallow depth. Thus, this population could be submitted to an ecological risk. |
| first_indexed | 2024-03-10T15:17:55Z |
| format | Article |
| id | doaj.art-84936cd560dd45eb98f622db8db7195b |
| institution | Directory Open Access Journal |
| issn | 2039-4705 2039-4713 |
| language | English |
| last_indexed | 2024-03-10T15:17:55Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Xenobiotics |
| spelling | doaj.art-84936cd560dd45eb98f622db8db7195b2023-11-20T18:44:03ZengMDPI AGJournal of Xenobiotics2039-47052039-47132020-10-01102143510.3390/jox10020004An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine EstuaryElodie Borcier0Grégory Charrier1Jérôme Couteau2Géraldine Maillet3Fabienne Le Grand4Antoine Bideau5Matthieu Waeles6Stéphane Le Floch7Rachid Amara8Vianney Pichereau9Jean Laroche10Laboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceTOXEM, 12 rue des 4 saisons, 76290 Montivilliers, FranceTOXEM, 12 rue des 4 saisons, 76290 Montivilliers, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceCEDRE, 715 rue Alain Colas, 29200 Brest, FranceLaboratoire d’Océanologie et Géosciences UMR 8187, Université du Littoral, 28 Avenue du Maréchal Foch, 62930 Wimereux, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceLaboratoire des Sciences de l’Environnement Marin, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, UMR 6539 CNRS/UBO/IRD/Ifremer, Place Nicolas Copernic, 29280 Plouzané, FranceThe objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders (<i>Platichthys flesus</i>) collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs. Douarnenez fish. The Seine fish displaying a reduced capacity to manage the oxidative stress and a higher energetic metabolism. An increase in the content of muscle membrane phospholipids (sphingomyelin, phosphatidylserine, free sterols) was detected in the Seine vs. Douarnenez fish. The data integration allowed to hypothesize relationships between membrane phospholipids, xenobiotic metabolism, bioenergetics, and antioxidant defence. The genetic diversity considering neutral markers was maintained in the heavily polluted Seine population compared with the Douarnenez population. Finally, we suggest that the high physiological cost of tolerance to toxicants in the Seine flounder population could compromise its capacity to respond in the future to an additional stressor like warming waters in shallow depth. Thus, this population could be submitted to an ecological risk.https://www.mdpi.com/2039-4713/10/2/4<i>Platichthys flesus</i>estuariesbiomarkersbioenergeticspolar lipidspopulation genetics |
| spellingShingle | Elodie Borcier Grégory Charrier Jérôme Couteau Géraldine Maillet Fabienne Le Grand Antoine Bideau Matthieu Waeles Stéphane Le Floch Rachid Amara Vianney Pichereau Jean Laroche An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary Journal of Xenobiotics <i>Platichthys flesus</i> estuaries biomarkers bioenergetics polar lipids population genetics |
| title | An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary |
| title_full | An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary |
| title_fullStr | An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary |
| title_full_unstemmed | An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary |
| title_short | An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary |
| title_sort | integrated biomarker approach using flounder to improve chemical risk assessments in the heavily polluted seine estuary |
| topic | <i>Platichthys flesus</i> estuaries biomarkers bioenergetics polar lipids population genetics |
| url | https://www.mdpi.com/2039-4713/10/2/4 |
| work_keys_str_mv | AT elodieborcier anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT gregorycharrier anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT jeromecouteau anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT geraldinemaillet anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT fabiennelegrand anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT antoinebideau anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT matthieuwaeles anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT stephanelefloch anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT rachidamara anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT vianneypichereau anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT jeanlaroche anintegratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT elodieborcier integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT gregorycharrier integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT jeromecouteau integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT geraldinemaillet integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT fabiennelegrand integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT antoinebideau integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT matthieuwaeles integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT stephanelefloch integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT rachidamara integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT vianneypichereau integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary AT jeanlaroche integratedbiomarkerapproachusingfloundertoimprovechemicalriskassessmentsintheheavilypollutedseineestuary |