TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells
Background: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-a. Methods: Caco-2...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2013-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2013/1452-82581302116L.pdf |
| _version_ | 1819130870109306880 |
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| author | Liu Dongyan Wang Si Jiang Teng Ding Peng |
| author_facet | Liu Dongyan Wang Si Jiang Teng Ding Peng |
| author_sort | Liu Dongyan |
| collection | DOAJ |
| description | Background: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-a. Methods: Caco-2 cells were cotreated with TNF-α plus either Wortmannin, which inhibits PI3K, or Rapamycin, which inhibits mTOR. The expression of SC and pIgR mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR. Results: Wortmannin and Rapamycin decreased the fraction of cells expressing SC, as well as the total expression of SC protein and pIgR mRNA. Conclusions: TNF-α regulation of SC expression is mediated through a PI3K/AKT/mTOR signaling pathway in Caco-2 cells. |
| first_indexed | 2024-12-22T09:06:28Z |
| format | Article |
| id | doaj.art-8494cbceb5eb42738007c14f527db15f |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-22T09:06:28Z |
| publishDate | 2013-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-8494cbceb5eb42738007c14f527db15f2022-12-21T18:31:35ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662013-01-013221161201452-82581302116LTNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cellsLiu Dongyan0Wang Si1Jiang Teng2Ding Peng3China Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, Shenyang City, Liaoning Province, ChinaChina Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, Shenyang City, Liaoning Province, ChinaChina Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, Shenyang City, Liaoning Province, ChinaChina Medical University Affiliated Shengjing Hospital Research Center and Key Laboratory of Congenital Malformation Research, Ministry of Health, Shenyang City, Liaoning Province, ChinaBackground: Secretory component (SC) is the extracellular component of the polymeric immunoglobulin receptor (pIgR) that functions as a receptor for externally secreted polymeric immunoglobulins such as secretory IgA. SC expression is regulated by a signaling pathway involving TNF-a. Methods: Caco-2 cells were cotreated with TNF-α plus either Wortmannin, which inhibits PI3K, or Rapamycin, which inhibits mTOR. The expression of SC and pIgR mRNA were assessed by immunocytochemistry, Western blotting, and Quantitative real-time PCR. Results: Wortmannin and Rapamycin decreased the fraction of cells expressing SC, as well as the total expression of SC protein and pIgR mRNA. Conclusions: TNF-α regulation of SC expression is mediated through a PI3K/AKT/mTOR signaling pathway in Caco-2 cells.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2013/1452-82581302116L.pdfsecretory componentpolymeric ig receptortumor necrosis factor-alphaphosphoinositide 3-kinasemammalian target of rapamycin |
| spellingShingle | Liu Dongyan Wang Si Jiang Teng Ding Peng TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells Journal of Medical Biochemistry secretory component polymeric ig receptor tumor necrosis factor-alpha phosphoinositide 3-kinase mammalian target of rapamycin |
| title | TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells |
| title_full | TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells |
| title_fullStr | TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells |
| title_full_unstemmed | TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells |
| title_short | TNF-α mediated secretory component is positively regulated by PI3K/AKT/mTOR in Caco-2 cells |
| title_sort | tnf α mediated secretory component is positively regulated by pi3k akt mtor in caco 2 cells |
| topic | secretory component polymeric ig receptor tumor necrosis factor-alpha phosphoinositide 3-kinase mammalian target of rapamycin |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2013/1452-82581302116L.pdf |
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