Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma
Abstract Background Hepatocellular carcinoma (HCC) ranks as the third most common cause of cancer related death globally, representing a substantial challenge to global healthcare systems. In China, the primary risk factor for HCC is the hepatitis B virus (HBV). Aberrant serum glycoconjugate levels...
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | Cell Communication and Signaling |
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| Online Access: | https://doi.org/10.1186/s12964-024-01569-y |
| _version_ | 1797219673964544000 |
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| author | Zhuo Li Na Zhang Zewen Dong Xin Wang Jian Zhou Juan Gao Yunyun Yang Jing Li Feng Guan Yue Zhou Zengqi Tan |
| author_facet | Zhuo Li Na Zhang Zewen Dong Xin Wang Jian Zhou Juan Gao Yunyun Yang Jing Li Feng Guan Yue Zhou Zengqi Tan |
| author_sort | Zhuo Li |
| collection | DOAJ |
| description | Abstract Background Hepatocellular carcinoma (HCC) ranks as the third most common cause of cancer related death globally, representing a substantial challenge to global healthcare systems. In China, the primary risk factor for HCC is the hepatitis B virus (HBV). Aberrant serum glycoconjugate levels have long been linked to the progression of HBV-associated HCC (HBV-HCC). Nevertheless, few study systematically explored the dysregulation of glycoconjugates in the progression of HBV-associated HCC and their potency as the diagnostic and prognostic biomarker. Methods An integrated strategy that combined transcriptomics, glycomics, and glycoproteomics was employed to comprehensively investigate the dynamic alterations in glyco-genes, N-glycans, and glycoproteins in the progression of HBV- HCC. Results Bioinformatic analysis of Gene Expression Omnibus (GEO) datasets uncovered dysregulation of fucosyltransferases (FUTs) in liver tissues from HCC patients compared to adjacent tissues. Glycomic analysis indicated an elevated level of fucosylated N-glycans, especially a progressive increase in fucosylation levels on IgA1 and IgG2 determined by glycoproteomic analysis. Conclusions The findings indicate that the abnormal fucosylation plays a pivotal role in the progression of HBV-HCC. Systematic and integrative multi-omic analysis is anticipated to facilitate the discovery of aberrant glycoconjugates in tumor progression. |
| first_indexed | 2024-04-24T12:37:23Z |
| format | Article |
| id | doaj.art-849665dc2f684550950a3159a4673049 |
| institution | Directory Open Access Journal |
| issn | 1478-811X |
| language | English |
| last_indexed | 2024-04-24T12:37:23Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Communication and Signaling |
| spelling | doaj.art-849665dc2f684550950a3159a46730492024-04-07T11:25:21ZengBMCCell Communication and Signaling1478-811X2024-04-0122111510.1186/s12964-024-01569-yIntegrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinomaZhuo Li0Na Zhang1Zewen Dong2Xin Wang3Jian Zhou4Juan Gao5Yunyun Yang6Jing Li7Feng Guan8Yue Zhou9Zengqi Tan10Department of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical UniversityDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical UniversityKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest UniversityDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical UniversityDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical UniversityDepartment of Laboratory Medicine, The First Affiliated Hospital of Xi’an Medical UniversityKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest UniversityKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest UniversityKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest UniversityKey Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Provincial Key Laboratory of Biotechnology, College of Life Sciences, Northwest UniversityInstitute of Hematology, Provincial Key Laboratory of Biotechnology, School of Medicine, Northwest UniversityAbstract Background Hepatocellular carcinoma (HCC) ranks as the third most common cause of cancer related death globally, representing a substantial challenge to global healthcare systems. In China, the primary risk factor for HCC is the hepatitis B virus (HBV). Aberrant serum glycoconjugate levels have long been linked to the progression of HBV-associated HCC (HBV-HCC). Nevertheless, few study systematically explored the dysregulation of glycoconjugates in the progression of HBV-associated HCC and their potency as the diagnostic and prognostic biomarker. Methods An integrated strategy that combined transcriptomics, glycomics, and glycoproteomics was employed to comprehensively investigate the dynamic alterations in glyco-genes, N-glycans, and glycoproteins in the progression of HBV- HCC. Results Bioinformatic analysis of Gene Expression Omnibus (GEO) datasets uncovered dysregulation of fucosyltransferases (FUTs) in liver tissues from HCC patients compared to adjacent tissues. Glycomic analysis indicated an elevated level of fucosylated N-glycans, especially a progressive increase in fucosylation levels on IgA1 and IgG2 determined by glycoproteomic analysis. Conclusions The findings indicate that the abnormal fucosylation plays a pivotal role in the progression of HBV-HCC. Systematic and integrative multi-omic analysis is anticipated to facilitate the discovery of aberrant glycoconjugates in tumor progression.https://doi.org/10.1186/s12964-024-01569-yHBV-associated HCCTranscriptomicsGlycomicsGlycoproteomicsFucosylation |
| spellingShingle | Zhuo Li Na Zhang Zewen Dong Xin Wang Jian Zhou Juan Gao Yunyun Yang Jing Li Feng Guan Yue Zhou Zengqi Tan Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma Cell Communication and Signaling HBV-associated HCC Transcriptomics Glycomics Glycoproteomics Fucosylation |
| title | Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma |
| title_full | Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma |
| title_fullStr | Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma |
| title_full_unstemmed | Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma |
| title_short | Integrating transcriptomics, glycomics and glycoproteomics to characterize hepatitis B virus-associated hepatocellular carcinoma |
| title_sort | integrating transcriptomics glycomics and glycoproteomics to characterize hepatitis b virus associated hepatocellular carcinoma |
| topic | HBV-associated HCC Transcriptomics Glycomics Glycoproteomics Fucosylation |
| url | https://doi.org/10.1186/s12964-024-01569-y |
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