Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes
Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisa...
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Format: | Article |
Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-06-01
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Series: | Memorias do Instituto Oswaldo Cruz |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400468&lng=en&tlng=en |
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author | S Andrea Moreno Mayerly Nava |
author_facet | S Andrea Moreno Mayerly Nava |
author_sort | S Andrea Moreno |
collection | DOAJ |
description | Trypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BSF) of T. b. brucei: (i) fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hexokinase, phosphofructokinase, glucose-6-phosphate isomerase, phosphoglycerate kinase, triosephosphate isomerase (glycolytic enzymes) and glycerol-3-phosphate dehydrogenase (a glycolysis-auxiliary enzyme) in glycosomes, (ii) enolase, phosphoglycerate mutase, pyruvate kinase (glycolytic enzymes) and a GAPDH isoenzyme in the cytosol, (iii) malate dehydrogenase in cytosol and (iv) glucose-6-phosphate dehydrogenase in both glycosomes and the cytosol. Specific enzymatic activities also suggest that T. evansi is alike to the BSF of T. b. brucei in glycolytic flux, which is much faster than the pentose phosphate pathway flux, and in the involvement of cytosolic GAPDH in the NAD+/NADH balance. These similarities were expected based on the close phylogenetic relationship of both parasites. |
first_indexed | 2024-03-12T07:34:14Z |
format | Article |
id | doaj.art-84989b1b05324efab862d4a97f94d76f |
institution | Directory Open Access Journal |
issn | 1678-8060 |
language | English |
last_indexed | 2024-03-12T07:34:14Z |
publishDate | 2015-06-01 |
publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
record_format | Article |
series | Memorias do Instituto Oswaldo Cruz |
spelling | doaj.art-84989b1b05324efab862d4a97f94d76f2023-09-02T21:33:31ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-06-01110446847510.1590/0074-02760150024S0074-02762015000400468Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymesS Andrea MorenoMayerly NavaTrypanosoma evansi, which causes surra, is descended from Trypanosoma brucei brucei, which causes nagana. Although both parasites are presumed to be metabolically similar, insufficient knowledge of T. evansi precludes a full comparison. Herein, we provide the first report on the subcellular localisation of the glycolytic enzymes in T. evansi, which is a alike to that of the bloodstream form (BSF) of T. b. brucei: (i) fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), hexokinase, phosphofructokinase, glucose-6-phosphate isomerase, phosphoglycerate kinase, triosephosphate isomerase (glycolytic enzymes) and glycerol-3-phosphate dehydrogenase (a glycolysis-auxiliary enzyme) in glycosomes, (ii) enolase, phosphoglycerate mutase, pyruvate kinase (glycolytic enzymes) and a GAPDH isoenzyme in the cytosol, (iii) malate dehydrogenase in cytosol and (iv) glucose-6-phosphate dehydrogenase in both glycosomes and the cytosol. Specific enzymatic activities also suggest that T. evansi is alike to the BSF of T. b. brucei in glycolytic flux, which is much faster than the pentose phosphate pathway flux, and in the involvement of cytosolic GAPDH in the NAD+/NADH balance. These similarities were expected based on the close phylogenetic relationship of both parasites.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400468&lng=en&tlng=endetrended correspondence analysisdifferential centrifugationdigitonin permeabilisationhaemoparasitesisopycnic ultracentrifugationsurra |
spellingShingle | S Andrea Moreno Mayerly Nava Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes Memorias do Instituto Oswaldo Cruz detrended correspondence analysis differential centrifugation digitonin permeabilisation haemoparasites isopycnic ultracentrifugation surra |
title | Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
title_full | Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
title_fullStr | Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
title_full_unstemmed | Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
title_short | Trypanosoma evansi is alike to Trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
title_sort | trypanosoma evansi is alike to trypanosoma brucei brucei in the subcellular localisation of glycolytic enzymes |
topic | detrended correspondence analysis differential centrifugation digitonin permeabilisation haemoparasites isopycnic ultracentrifugation surra |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000400468&lng=en&tlng=en |
work_keys_str_mv | AT sandreamoreno trypanosomaevansiisaliketotrypanosomabruceibruceiinthesubcellularlocalisationofglycolyticenzymes AT mayerlynava trypanosomaevansiisaliketotrypanosomabruceibruceiinthesubcellularlocalisationofglycolyticenzymes |