| Summary: | In this study, we prepared silver nanoparticles (AgNPs) employing ultrasound and chitosan (CS) in the presence of Pistacia atlantica aqueous extract as a non-toxic stabilizing and reducing agent for capping AgNPs with CS in a simple single-step process. The reduction of Ag+ ions into Ag0NPs was indicated by a visual transform in color. UV-Vis studies revealed that the characteristic surface plasmon resonance band appeared at around 460 nm. FT-IR, TEM, SEM, EDX, elemental mapping, XRD, and UV/Vis analytical methods were employed to characterize the AgNPs@CS nanocomposite. TEM study revealed nanoflowers-shaped NPs having various particle sizes. In the oncological part of this research, the status of normal and gastric cancer cell lines (MKN45, MKN28, NCI-N87, SNU-16, SNU-5, SNU-1, AGS, and KATO III) against the proposed nanomaterial were determined. The IC50 of AgNPs was 265, 304, 325, 127, 297, 318, 125, and 114 µg/mL against MKN45, MKN28, NCI-N87, SNU-16, SNU-5, SNU-1, AGS, and KATO III, respectively. It seems that the prepared NPs have stopped the growth of gastric cancer cells and the recent cancer cells have been removed with a high concentration of NPs. DPPH test results indicated removing 50% radicals by nanocomposite and butylated hydroxytoluene in the doses of 183 and 45 μg/mL.
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