Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue.
The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS wer...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4526652?pdf=render |
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| author | Carlos F Santos Ana C Morandini Thiago J Dionísio Flávio A Faria Marta C Lima Caio M Figueiredo Bella L Colombini-Ishikiriama Carla R Sipert Rubens P Maciel Ana P Akashi Gabriela P Souza Gustavo P Garlet Camila O Rodini Sandra L Amaral Christiane Becari Maria C Salgado Eduardo B Oliveira Isaac Matus Daniela N Didier Andrew S Greene |
| author_facet | Carlos F Santos Ana C Morandini Thiago J Dionísio Flávio A Faria Marta C Lima Caio M Figueiredo Bella L Colombini-Ishikiriama Carla R Sipert Rubens P Maciel Ana P Akashi Gabriela P Souza Gustavo P Garlet Camila O Rodini Sandra L Amaral Christiane Becari Maria C Salgado Eduardo B Oliveira Isaac Matus Daniela N Didier Andrew S Greene |
| author_sort | Carlos F Santos |
| collection | DOAJ |
| description | The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression were found between healthy and diseased gingiva. However, in inflamed tissue the immunoreactivity was greater for the AT1R compared to AT2R in fibroblasts. When compared to healthy tissue, ACE activity was increased in human gingiva from volunteers with gingivitis. Human-gingiva homogenates generated Ang II, Ang 1-9 and Ang 1-7 when incubated with precursors. In gingiva homogenates, Ang II formation from Ang I was nearly abolished only when captopril and chymostatin were combined. Ang 1-7 formation was significantly greater when human gingiva homogenates were incubated with chymostatin alone compared to incubation without any inhibitor, only captopril, or captopril and chymostatin. In rat gingiva, RAS components were also found; their expression was not different between healthy and experimentally induced periodontitis (EP) groups. However, renin inhibition (aliskiren) and an AT1R antagonist (losartan) significantly blocked EP-alveolar-bone loss in rats. Collectively, these data are consistent with the hypothesis that a local RAS system is not only present but is also functional in both human and rat periodontal tissue. Furthermore, blocking AT1R and renin can significantly prevent periodontal bone loss induced by EP in rats. |
| first_indexed | 2024-12-21T01:58:15Z |
| format | Article |
| id | doaj.art-8498f29ae5354bff93694f77bf807b57 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T01:58:15Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8498f29ae5354bff93694f77bf807b572022-12-21T19:19:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01108e013460110.1371/journal.pone.0134601Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue.Carlos F SantosAna C MorandiniThiago J DionísioFlávio A FariaMarta C LimaCaio M FigueiredoBella L Colombini-IshikiriamaCarla R SipertRubens P MacielAna P AkashiGabriela P SouzaGustavo P GarletCamila O RodiniSandra L AmaralChristiane BecariMaria C SalgadoEduardo B OliveiraIsaac MatusDaniela N DidierAndrew S GreeneThe initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression were found between healthy and diseased gingiva. However, in inflamed tissue the immunoreactivity was greater for the AT1R compared to AT2R in fibroblasts. When compared to healthy tissue, ACE activity was increased in human gingiva from volunteers with gingivitis. Human-gingiva homogenates generated Ang II, Ang 1-9 and Ang 1-7 when incubated with precursors. In gingiva homogenates, Ang II formation from Ang I was nearly abolished only when captopril and chymostatin were combined. Ang 1-7 formation was significantly greater when human gingiva homogenates were incubated with chymostatin alone compared to incubation without any inhibitor, only captopril, or captopril and chymostatin. In rat gingiva, RAS components were also found; their expression was not different between healthy and experimentally induced periodontitis (EP) groups. However, renin inhibition (aliskiren) and an AT1R antagonist (losartan) significantly blocked EP-alveolar-bone loss in rats. Collectively, these data are consistent with the hypothesis that a local RAS system is not only present but is also functional in both human and rat periodontal tissue. Furthermore, blocking AT1R and renin can significantly prevent periodontal bone loss induced by EP in rats.http://europepmc.org/articles/PMC4526652?pdf=render |
| spellingShingle | Carlos F Santos Ana C Morandini Thiago J Dionísio Flávio A Faria Marta C Lima Caio M Figueiredo Bella L Colombini-Ishikiriama Carla R Sipert Rubens P Maciel Ana P Akashi Gabriela P Souza Gustavo P Garlet Camila O Rodini Sandra L Amaral Christiane Becari Maria C Salgado Eduardo B Oliveira Isaac Matus Daniela N Didier Andrew S Greene Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. PLoS ONE |
| title | Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. |
| title_full | Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. |
| title_fullStr | Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. |
| title_full_unstemmed | Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. |
| title_short | Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue. |
| title_sort | functional local renin angiotensin system in human and rat periodontal tissue |
| url | http://europepmc.org/articles/PMC4526652?pdf=render |
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