Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway
Objective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2019-01-01
|
| Series: | Asian Pacific Journal of Tropical Medicine |
| Subjects: | |
| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=59;epage=68;aulast=Wu |
| _version_ | 1819079215640739840 |
|---|---|
| author | Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu |
| author_facet | Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu |
| author_sort | Wei Wu |
| collection | DOAJ |
| description | Objective: To investigate the effect of memantine on Parkinson’s disease cell models.
Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine.
Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway.
Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway. |
| first_indexed | 2024-12-21T19:25:27Z |
| format | Article |
| id | doaj.art-84992b8131484affaf1072c72c96c670 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-21T19:25:27Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-84992b8131484affaf1072c72c96c6702022-12-21T18:52:50ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462019-01-011214596810.4103/1995-7645.271981Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathwayWei WuRui WangYong-Mei FuJie ZhouHai-Chao HuangWei-Wang GuObjective: To investigate the effect of memantine on Parkinson’s disease cell models. Methods: Parkinson’s disease cell models were established using PC12 cells incubated with 6-hydroxydopamine (6-OHDA). Flow cytometry and microscopy were used to investigate the apoptotic process and the percentage of different apoptotic stages. PC12 cells were infected with lentiviral vectors to knockdown Nur77. Western blotting was used to detect the expression of Nur77 and caspase -3, -8, -9, -12 in PC12 cells withdifferent concentrations of 6-OHDA or 6-OHDA+memantine. Results: 6-OHDA led to apoptosis PC12 cells, and increased the expression of Nur77 and caspases. Memantine significantly inhibited 6-OHDA-induced apoptosis of PC12 cells. Meanwhile, memantine could mitigate apoptosis of PC12 cells by regulating the Nur77 and caspase pathway. Conclusions: Memantine has a protective effect on the PC12 cell model via regulating the Nur77 and caspases pathway.http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=59;epage=68;aulast=Wuparkinson’s disease6-ohdanur77memantineapoptosis |
| spellingShingle | Wei Wu Rui Wang Yong-Mei Fu Jie Zhou Hai-Chao Huang Wei-Wang Gu Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway Asian Pacific Journal of Tropical Medicine parkinson’s disease 6-ohda nur77 memantine apoptosis |
| title | Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
| title_full | Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
| title_fullStr | Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
| title_full_unstemmed | Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
| title_short | Memantine inhibits 6-OHDA-induced apoptosis PC12 cells via the Nurr77 and caspase pathway |
| title_sort | memantine inhibits 6 ohda induced apoptosis pc12 cells via the nurr77 and caspase pathway |
| topic | parkinson’s disease 6-ohda nur77 memantine apoptosis |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=14;spage=59;epage=68;aulast=Wu |
| work_keys_str_mv | AT weiwu memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway AT ruiwang memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway AT yongmeifu memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway AT jiezhou memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway AT haichaohuang memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway AT weiwanggu memantineinhibits6ohdainducedapoptosispc12cellsviathenurr77andcaspasepathway |