Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>)
White button mushroom—<i>Agaricus bisporus</i> (J.E.Lange) Imbach—is among the most popular cultivated mushrooms worldwide. The most serious challenge in industrial mushroom production is the green mold disease caused by <i>Trichoderma</i> species. Our aim was to isolate and...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/2/467 |
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| author | Rita Büchner Mónika Vörös Henrietta Allaga András Varga Attila Bartal András Szekeres Sarolta Varga Judit Bajzát Nóra Bakos-Barczi András Misz Csaba Csutorás Lóránt Hatvani Csaba Vágvölgyi László Kredics |
| author_facet | Rita Büchner Mónika Vörös Henrietta Allaga András Varga Attila Bartal András Szekeres Sarolta Varga Judit Bajzát Nóra Bakos-Barczi András Misz Csaba Csutorás Lóránt Hatvani Csaba Vágvölgyi László Kredics |
| author_sort | Rita Büchner |
| collection | DOAJ |
| description | White button mushroom—<i>Agaricus bisporus</i> (J.E.Lange) Imbach—is among the most popular cultivated mushrooms worldwide. The most serious challenge in industrial mushroom production is the green mold disease caused by <i>Trichoderma</i> species. Our aim was to isolate and examine bacterial strains from mushroom casing material for their potential use as biocontrol agents. Twenty-seven bacterial strains were isolated and tested against mold pathogens of white button mushroom. The <i>Bacillus velezensis</i> strain SZMC 25431 was selected for further examination and tested under simulated <i>Agaricus</i> cultivation conditions against <i>T. aggressivum</i> SZMC 23834 in a 1200-L Fitotron SGC120 standard plant growth chamber. Our results showed that the bacterial treatment was effective against the pathogen in all cases, but the best results were achieved at an application concentration of 10<sup>5</sup> cells mL<sup>−1</sup>. Industrial-scale experiments were also carried out in <i>Agaricus</i> growing houses with a bearing surface of 480 m<sup>2</sup>: the bacterial suspension was mixed in water tanks applied for daily irrigation. The results suggest that the bacterial treatment may even increase the crop yield of <i>A. bisporus</i>. Based on our results, we concluded that the selected <i>B. velezensis</i> strain may potentially be used for biological and integrated treatment in <i>Agaricus</i> cultivation. |
| first_indexed | 2024-03-09T22:49:29Z |
| format | Article |
| id | doaj.art-8499fc299d774fbaaf5408c4bbea7012 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T22:49:29Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-8499fc299d774fbaaf5408c4bbea70122023-11-23T18:22:33ZengMDPI AGAgronomy2073-43952022-02-0112246710.3390/agronomy12020467Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>)Rita Büchner0Mónika Vörös1Henrietta Allaga2András Varga3Attila Bartal4András Szekeres5Sarolta Varga6Judit Bajzát7Nóra Bakos-Barczi8András Misz9Csaba Csutorás10Lóránt Hatvani11Csaba Vágvölgyi12László Kredics13Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryÚj Champignons Ltd., Bartók Béla út 162., 1224 Budapest, HungaryÚj Champignons Ltd., Bartók Béla út 162., 1224 Budapest, HungaryÚj Champignons Ltd., Bartók Béla út 162., 1224 Budapest, HungaryDepartment of Chemistry and Food Chemistry, Institute of Food Sciences, Eszterházy Károly Catholic University, Leányka utca 4-8., 3300 Eger, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryDepartment of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52., 6726 Szeged, HungaryWhite button mushroom—<i>Agaricus bisporus</i> (J.E.Lange) Imbach—is among the most popular cultivated mushrooms worldwide. The most serious challenge in industrial mushroom production is the green mold disease caused by <i>Trichoderma</i> species. Our aim was to isolate and examine bacterial strains from mushroom casing material for their potential use as biocontrol agents. Twenty-seven bacterial strains were isolated and tested against mold pathogens of white button mushroom. The <i>Bacillus velezensis</i> strain SZMC 25431 was selected for further examination and tested under simulated <i>Agaricus</i> cultivation conditions against <i>T. aggressivum</i> SZMC 23834 in a 1200-L Fitotron SGC120 standard plant growth chamber. Our results showed that the bacterial treatment was effective against the pathogen in all cases, but the best results were achieved at an application concentration of 10<sup>5</sup> cells mL<sup>−1</sup>. Industrial-scale experiments were also carried out in <i>Agaricus</i> growing houses with a bearing surface of 480 m<sup>2</sup>: the bacterial suspension was mixed in water tanks applied for daily irrigation. The results suggest that the bacterial treatment may even increase the crop yield of <i>A. bisporus</i>. Based on our results, we concluded that the selected <i>B. velezensis</i> strain may potentially be used for biological and integrated treatment in <i>Agaricus</i> cultivation.https://www.mdpi.com/2073-4395/12/2/467<i>Agaricus bisporus</i><i>Bacillus velezensis</i>biocontrolgreen mold<i>Trichoderma aggressivum</i> |
| spellingShingle | Rita Büchner Mónika Vörös Henrietta Allaga András Varga Attila Bartal András Szekeres Sarolta Varga Judit Bajzát Nóra Bakos-Barczi András Misz Csaba Csutorás Lóránt Hatvani Csaba Vágvölgyi László Kredics Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) Agronomy <i>Agaricus bisporus</i> <i>Bacillus velezensis</i> biocontrol green mold <i>Trichoderma aggressivum</i> |
| title | Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) |
| title_full | Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) |
| title_fullStr | Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) |
| title_full_unstemmed | Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) |
| title_short | Selection and Characterization of a <i>Bacillus</i> Strain for Potential Application in Industrial Production of White Button Mushroom (<i>Agaricus bisporus</i>) |
| title_sort | selection and characterization of a i bacillus i strain for potential application in industrial production of white button mushroom i agaricus bisporus i |
| topic | <i>Agaricus bisporus</i> <i>Bacillus velezensis</i> biocontrol green mold <i>Trichoderma aggressivum</i> |
| url | https://www.mdpi.com/2073-4395/12/2/467 |
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