miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis
Abstract Background Deep venous thrombosis (DVT) of lower extremities is a common thrombotic disease, occurring either in isolation or as a complication of other diseases or procedures. MiR-21 is one of important microRNAs which play critical role in various cellular function. This study aim to dete...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-08-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12967-019-2015-z |
| _version_ | 1818160038391316480 |
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| author | Xiaolong Du Lei Hong Lili Sun Hongfei Sang Aiming Qian Wendong Li Hao Zhuang Huoqi Liang Dandan Song Chenglong Li Wenbin Wang Xiaoqiang Li |
| author_facet | Xiaolong Du Lei Hong Lili Sun Hongfei Sang Aiming Qian Wendong Li Hao Zhuang Huoqi Liang Dandan Song Chenglong Li Wenbin Wang Xiaoqiang Li |
| author_sort | Xiaolong Du |
| collection | DOAJ |
| description | Abstract Background Deep venous thrombosis (DVT) of lower extremities is a common thrombotic disease, occurring either in isolation or as a complication of other diseases or procedures. MiR-21 is one of important microRNAs which play critical role in various cellular function. This study aim to determine the effect of miR-21 on endothelial progenitor cells (EPCs) and its role in predicting prognosis of DVT. Methods EPCs was isolated from DVT models and control subjects. miR-21 expression was confirmed by RT-PCR. Potential target mRNA was predicted by bioinformatics analysis. EPCs biological functions were examined by CCK-8 and tube formation assay. Besides, miR-21 expression was determined in DVT patients to investigate the correlation between miR-21 expression and prognosis of DVT. Cox proportional hazard regression analyses were also performed to reveal the risk factors associated with prognosis. Results Here, we found miR-21 was downregulated in EPCs of DVT model rats. Increased miR-21 expression promoted proliferation and angiogenesis of EPCs. Moreover, we demonstrated that FASLG was a target of miR-21 and revealed that FASLG knockdown inhibited function of EPCs. Upregulation of miR-21 led to thrombus resolution in a rat model of venous thrombosis. In addition, lower expression level of miR-21 in DVT patients was associated with an increase of recurrent DVT and post thrombotic syndrome (PTS). Furthermore, Cox proportional hazard regression analyses demonstrated miR-21 expression level as an independent predictor of recurrence of DVT. Conclusions Our data revealed a role of miR-21 in regulating biological function of EPCs and could be a predictor for recurrent DVT or PTS. |
| first_indexed | 2024-12-11T15:55:31Z |
| format | Article |
| id | doaj.art-849dce0560d946e8abe4baa7fd68b74c |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-11T15:55:31Z |
| publishDate | 2019-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-849dce0560d946e8abe4baa7fd68b74c2022-12-22T00:59:28ZengBMCJournal of Translational Medicine1479-58762019-08-0117111310.1186/s12967-019-2015-zmiR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosisXiaolong Du0Lei Hong1Lili Sun2Hongfei Sang3Aiming Qian4Wendong Li5Hao Zhuang6Huoqi Liang7Dandan Song8Chenglong Li9Wenbin Wang10Xiaoqiang Li11Department of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical SchoolDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical SchoolDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical SchoolDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical SchoolDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of Vascular Surgery, The Second Affiliated Hospital of Soochow UniversityDepartment of General Surgery, The Fourth Affiliated Hospital of Anhui Medical UniversityDepartment of Vascular Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical SchoolAbstract Background Deep venous thrombosis (DVT) of lower extremities is a common thrombotic disease, occurring either in isolation or as a complication of other diseases or procedures. MiR-21 is one of important microRNAs which play critical role in various cellular function. This study aim to determine the effect of miR-21 on endothelial progenitor cells (EPCs) and its role in predicting prognosis of DVT. Methods EPCs was isolated from DVT models and control subjects. miR-21 expression was confirmed by RT-PCR. Potential target mRNA was predicted by bioinformatics analysis. EPCs biological functions were examined by CCK-8 and tube formation assay. Besides, miR-21 expression was determined in DVT patients to investigate the correlation between miR-21 expression and prognosis of DVT. Cox proportional hazard regression analyses were also performed to reveal the risk factors associated with prognosis. Results Here, we found miR-21 was downregulated in EPCs of DVT model rats. Increased miR-21 expression promoted proliferation and angiogenesis of EPCs. Moreover, we demonstrated that FASLG was a target of miR-21 and revealed that FASLG knockdown inhibited function of EPCs. Upregulation of miR-21 led to thrombus resolution in a rat model of venous thrombosis. In addition, lower expression level of miR-21 in DVT patients was associated with an increase of recurrent DVT and post thrombotic syndrome (PTS). Furthermore, Cox proportional hazard regression analyses demonstrated miR-21 expression level as an independent predictor of recurrence of DVT. Conclusions Our data revealed a role of miR-21 in regulating biological function of EPCs and could be a predictor for recurrent DVT or PTS.http://link.springer.com/article/10.1186/s12967-019-2015-zmiR-21Endothelial progenitor cellsProliferationAngiogenesisVenous thrombosisPrognosis |
| spellingShingle | Xiaolong Du Lei Hong Lili Sun Hongfei Sang Aiming Qian Wendong Li Hao Zhuang Huoqi Liang Dandan Song Chenglong Li Wenbin Wang Xiaoqiang Li miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis Journal of Translational Medicine miR-21 Endothelial progenitor cells Proliferation Angiogenesis Venous thrombosis Prognosis |
| title | miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis |
| title_full | miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis |
| title_fullStr | miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis |
| title_full_unstemmed | miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis |
| title_short | miR-21 induces endothelial progenitor cells proliferation and angiogenesis via targeting FASLG and is a potential prognostic marker in deep venous thrombosis |
| title_sort | mir 21 induces endothelial progenitor cells proliferation and angiogenesis via targeting faslg and is a potential prognostic marker in deep venous thrombosis |
| topic | miR-21 Endothelial progenitor cells Proliferation Angiogenesis Venous thrombosis Prognosis |
| url | http://link.springer.com/article/10.1186/s12967-019-2015-z |
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