Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification
The upsurge of multiple drug resistance (MDR) bacteria substantially diminishes the effectiveness of antibiotic arsenal and therefore intensifies the rate of therapeutic failure. The major factor in MDR is efflux pump-mediated resistance. A unique pump can make bacteria withstand a wide range of str...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-08-01
|
| Series: | Journal of Pharmaceutical Analysis |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209517791930646X |
| _version_ | 1818438037824077824 |
|---|---|
| author | Armel Jackson Seukep Victor Kuete Lutfun Nahar Satyajit D. Sarker Mingquan Guo |
| author_facet | Armel Jackson Seukep Victor Kuete Lutfun Nahar Satyajit D. Sarker Mingquan Guo |
| author_sort | Armel Jackson Seukep |
| collection | DOAJ |
| description | The upsurge of multiple drug resistance (MDR) bacteria substantially diminishes the effectiveness of antibiotic arsenal and therefore intensifies the rate of therapeutic failure. The major factor in MDR is efflux pump-mediated resistance. A unique pump can make bacteria withstand a wide range of structurally diverse compounds. Therefore, their inhibition is a promising route to eliminate resistance phenomenon in bacteria. Phytochemicals are excellent alternatives as resistance-modifying agents. They can directly kill bacteria or interact with the crucial events of pathogenicity, thereby decreasing the ability of bacteria to develop resistance. Numerous botanicals display noteworthy efflux pumps inhibitory activities. Edible plants are of growing interest. Likewise, some plant families would be excellent sources of efflux pump inhibitors (EPIs) including Apocynaceae, Berberidaceae, Convolvulaceae, Cucurbitaceae, Fabaceae, Lamiaceae, and Zingiberaceae. Easily applicable methods for screening plant-derived EPIs include checkerboard synergy test, berberine uptake assay and ethidium bromide test. In silico high-throughput virtual detection can be evaluated as a criterion of excluding compounds with efflux substrate-like characteristics, thereby improving the selection process and extending the identification of EPIs. To ascertain the efflux activity inhibition, real-time PCR and quantitative mass spectrometry can be applied. This review emphasizes on efflux pumps and their roles in transmitting bacterial resistance and an update plant-derived EPIs and strategies for identification. |
| first_indexed | 2024-12-14T17:34:12Z |
| format | Article |
| id | doaj.art-849e20a7b80b42179b8edd90fabe261a |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-12-14T17:34:12Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-849e20a7b80b42179b8edd90fabe261a2022-12-21T22:53:02ZengElsevierJournal of Pharmaceutical Analysis2095-17792020-08-01104277290Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identificationArmel Jackson Seukep0Victor Kuete1Lutfun Nahar2Satyajit D. Sarker3Mingquan Guo4Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China; Unit of Research in Microbiology and Antimicrobial Substances/Laboratory of Cancer Research, Faculty of Science, Department of Biochemistry, University of Dschang, Dschang, CameroonUnit of Research in Microbiology and Antimicrobial Substances/Laboratory of Cancer Research, Faculty of Science, Department of Biochemistry, University of Dschang, Dschang, CameroonLaboratory of Growth Regulators, Institute of Experimental Botany ASCR & Palacký University, Šlechtitelů 27, 78371, Olomouc, Czech RepublicCentre for Natural Products Discovery, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool, L3 3AF, United KingdomKey Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China; Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan, China; Corresponding author. Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.The upsurge of multiple drug resistance (MDR) bacteria substantially diminishes the effectiveness of antibiotic arsenal and therefore intensifies the rate of therapeutic failure. The major factor in MDR is efflux pump-mediated resistance. A unique pump can make bacteria withstand a wide range of structurally diverse compounds. Therefore, their inhibition is a promising route to eliminate resistance phenomenon in bacteria. Phytochemicals are excellent alternatives as resistance-modifying agents. They can directly kill bacteria or interact with the crucial events of pathogenicity, thereby decreasing the ability of bacteria to develop resistance. Numerous botanicals display noteworthy efflux pumps inhibitory activities. Edible plants are of growing interest. Likewise, some plant families would be excellent sources of efflux pump inhibitors (EPIs) including Apocynaceae, Berberidaceae, Convolvulaceae, Cucurbitaceae, Fabaceae, Lamiaceae, and Zingiberaceae. Easily applicable methods for screening plant-derived EPIs include checkerboard synergy test, berberine uptake assay and ethidium bromide test. In silico high-throughput virtual detection can be evaluated as a criterion of excluding compounds with efflux substrate-like characteristics, thereby improving the selection process and extending the identification of EPIs. To ascertain the efflux activity inhibition, real-time PCR and quantitative mass spectrometry can be applied. This review emphasizes on efflux pumps and their roles in transmitting bacterial resistance and an update plant-derived EPIs and strategies for identification.http://www.sciencedirect.com/science/article/pii/S209517791930646XMultidrug-resistant bacteriaEfflux pump inhibitorsPlant secondary metabolitesEdible plantsEfflux activity assays |
| spellingShingle | Armel Jackson Seukep Victor Kuete Lutfun Nahar Satyajit D. Sarker Mingquan Guo Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification Journal of Pharmaceutical Analysis Multidrug-resistant bacteria Efflux pump inhibitors Plant secondary metabolites Edible plants Efflux activity assays |
| title | Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| title_full | Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| title_fullStr | Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| title_full_unstemmed | Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| title_short | Plant-derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| title_sort | plant derived secondary metabolites as the main source of efflux pump inhibitors and methods for identification |
| topic | Multidrug-resistant bacteria Efflux pump inhibitors Plant secondary metabolites Edible plants Efflux activity assays |
| url | http://www.sciencedirect.com/science/article/pii/S209517791930646X |
| work_keys_str_mv | AT armeljacksonseukep plantderivedsecondarymetabolitesasthemainsourceofeffluxpumpinhibitorsandmethodsforidentification AT victorkuete plantderivedsecondarymetabolitesasthemainsourceofeffluxpumpinhibitorsandmethodsforidentification AT lutfunnahar plantderivedsecondarymetabolitesasthemainsourceofeffluxpumpinhibitorsandmethodsforidentification AT satyajitdsarker plantderivedsecondarymetabolitesasthemainsourceofeffluxpumpinhibitorsandmethodsforidentification AT mingquanguo plantderivedsecondarymetabolitesasthemainsourceofeffluxpumpinhibitorsandmethodsforidentification |