Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital
The <i>Staphylococcus pseudintermedius</i> group (SIG) is an emerging threat in veterinary medicine, particularly methicillin-resistant (MRSP) isolates, which are frequently associated with multidrug resistance. Reliable identification of SIG members is critical to establish correct anti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/11/752 |
| _version_ | 1797549457563189248 |
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| author | Marta Pérez-Sancho Sergio Alvarez-Perez Teresa Garcia-Seco Marta Hernandez David Rodríguez-Lázaro Lucas Domínguez Marta Eulalia García Jose Luis Blanco |
| author_facet | Marta Pérez-Sancho Sergio Alvarez-Perez Teresa Garcia-Seco Marta Hernandez David Rodríguez-Lázaro Lucas Domínguez Marta Eulalia García Jose Luis Blanco |
| author_sort | Marta Pérez-Sancho |
| collection | DOAJ |
| description | The <i>Staphylococcus pseudintermedius</i> group (SIG) is an emerging threat in veterinary medicine, particularly methicillin-resistant (MRSP) isolates, which are frequently associated with multidrug resistance. Reliable identification of SIG members is critical to establish correct antimicrobial treatments. However, information on the molecular epidemiology and antimicrobial resistance patterns of MRSP in some regions is still limited. This study aimed to assess the antimicrobial resistance of SIG isolates recovered from animals at the Veterinary Teaching Hospital of Complutense University of Madrid (Spain) during a 10-year period (2007–2016). A total of 139 selected <i>Staphylococcus</i> isolates were subjected to species-level identification by different bioanalytical techniques (PCR, VITEK, MALDI-TOF) and subsequent antimicrobial susceptibility testing. Methicillin-resistant isolates (<i>n</i> = 20) were subjected to whole genome sequencing for further characterization of their antibiotic resistance determinants. Our results showed that there was a good correlation between PCR and MALDI-TOF identification, whereas VITEK showed very divergent results, thus confirming MALDI-TOF as a good alternative for species-level identification of coagulase-positive staphylococci. Notably, <i>S. pseudintermedius</i>, including the epidemic MRSP genotype ST71, was the only SIG species found among canine isolates. In addition, we found a high prevalence of multidrug resistance and resistance to fluoroquinolones, cephalosporins and macrolides. Finally, diverse genes associated with antibiotic resistance were detected among MRSP isolates, although the genetic basis of some of the resistant phenotypes (particularly to fluoroquinolones) could not be determined. In conclusion, our study reveals the circulation of MRSP in the veterinary setting in Spain, thus highlighting the emerging threat posed by this bacterial group and the need for further epidemiological surveillance. |
| first_indexed | 2024-03-10T15:14:55Z |
| format | Article |
| id | doaj.art-849faf8cbb21488baa47be527d10815e |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T15:14:55Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-849faf8cbb21488baa47be527d10815e2023-11-20T19:01:12ZengMDPI AGAntibiotics2079-63822020-10-0191175210.3390/antibiotics9110752Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University HospitalMarta Pérez-Sancho0Sergio Alvarez-Perez1Teresa Garcia-Seco2Marta Hernandez3David Rodríguez-Lázaro4Lucas Domínguez5Marta Eulalia García6Jose Luis Blanco7Department of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainVISAVET Health Surveillance Centre. Complutense University, 28040 Madrid, SpainLaboratorio de Biología Molecular y Microbiología, Instituto Tecnológico Agrario de Castilla y León, 47071 Valladolid, SpainÁrea de Microbiología, Departamento de Biotecnología y Ciencia de los Alimentos, Universidad de Burgos, 09001 Burgos, SpainDepartment of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainDepartment of Animal Health, Faculty of Veterinary Medicine, Complutense University of Madrid, 28040 Madrid, SpainThe <i>Staphylococcus pseudintermedius</i> group (SIG) is an emerging threat in veterinary medicine, particularly methicillin-resistant (MRSP) isolates, which are frequently associated with multidrug resistance. Reliable identification of SIG members is critical to establish correct antimicrobial treatments. However, information on the molecular epidemiology and antimicrobial resistance patterns of MRSP in some regions is still limited. This study aimed to assess the antimicrobial resistance of SIG isolates recovered from animals at the Veterinary Teaching Hospital of Complutense University of Madrid (Spain) during a 10-year period (2007–2016). A total of 139 selected <i>Staphylococcus</i> isolates were subjected to species-level identification by different bioanalytical techniques (PCR, VITEK, MALDI-TOF) and subsequent antimicrobial susceptibility testing. Methicillin-resistant isolates (<i>n</i> = 20) were subjected to whole genome sequencing for further characterization of their antibiotic resistance determinants. Our results showed that there was a good correlation between PCR and MALDI-TOF identification, whereas VITEK showed very divergent results, thus confirming MALDI-TOF as a good alternative for species-level identification of coagulase-positive staphylococci. Notably, <i>S. pseudintermedius</i>, including the epidemic MRSP genotype ST71, was the only SIG species found among canine isolates. In addition, we found a high prevalence of multidrug resistance and resistance to fluoroquinolones, cephalosporins and macrolides. Finally, diverse genes associated with antibiotic resistance were detected among MRSP isolates, although the genetic basis of some of the resistant phenotypes (particularly to fluoroquinolones) could not be determined. In conclusion, our study reveals the circulation of MRSP in the veterinary setting in Spain, thus highlighting the emerging threat posed by this bacterial group and the need for further epidemiological surveillance.https://www.mdpi.com/2079-6382/9/11/752SIG groupMALDI-TOFwhole genome sequencingantimicrobial resistance |
| spellingShingle | Marta Pérez-Sancho Sergio Alvarez-Perez Teresa Garcia-Seco Marta Hernandez David Rodríguez-Lázaro Lucas Domínguez Marta Eulalia García Jose Luis Blanco Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital Antibiotics SIG group MALDI-TOF whole genome sequencing antimicrobial resistance |
| title | Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital |
| title_full | Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital |
| title_fullStr | Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital |
| title_full_unstemmed | Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital |
| title_short | Antimicrobial Resistance of Coagulase-Positive <i>Staphylococcus</i> Isolates Recovered in a Veterinary University Hospital |
| title_sort | antimicrobial resistance of coagulase positive i staphylococcus i isolates recovered in a veterinary university hospital |
| topic | SIG group MALDI-TOF whole genome sequencing antimicrobial resistance |
| url | https://www.mdpi.com/2079-6382/9/11/752 |
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