Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study
The SARS-CoV-2 pandemic led to the development of various vaccines. The BNT162b2 mRNA vaccine was the first approved due to its efficacy in eliciting a humoral immunity response after the second dose. However, a decrease in the antibody concentration was observed over time. Therefore, the administra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/10/6/862 |
| _version_ | 1797481632134856704 |
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| author | Alessandra Panico Giambattista Lobreglio Francesco Bagordo Antonella Zizza Antonella De Donno Chiara Rosato Roberta Lazzari Michele Chicone Floriano Indino Virginia Recchia Pietro Alifano Tiziana Grassi |
| author_facet | Alessandra Panico Giambattista Lobreglio Francesco Bagordo Antonella Zizza Antonella De Donno Chiara Rosato Roberta Lazzari Michele Chicone Floriano Indino Virginia Recchia Pietro Alifano Tiziana Grassi |
| author_sort | Alessandra Panico |
| collection | DOAJ |
| description | The SARS-CoV-2 pandemic led to the development of various vaccines. The BNT162b2 mRNA vaccine was the first approved due to its efficacy in eliciting a humoral immunity response after the second dose. However, a decrease in the antibody concentration was observed over time. Therefore, the administration of a third dose was scheduled, primarily for frail people and workers of essential public activities. The aim of this study was to assess the level of antibodies against the spike (S) RBD of SARS-CoV-2 in healthcare workers before and after the third dose of BNT162b2 vaccine, according to sex, age, and the time interval between vaccine doses and tests. All 37 (12 males, 25 females, 19 < 50 years old, 18 ≥ 50 years old) healthcare workers recruited showed a consistent antibody titer increase after the third dose. Data analysis showed that the antibody concentration before the third dose significantly decreased as the time interval up to the test increased, and a significantly higher level was shown in young than older people. Cluster analysis revealed that young females had a higher antibody level than older females before the third dose (<i>p</i> < 0.05). This study indicated the benefit of the third dose of BNT162b2 vaccine and its effect on leveling up the humoral immune response. |
| first_indexed | 2024-03-09T22:17:21Z |
| format | Article |
| id | doaj.art-84a245cb9b144811b04124edb1d7139f |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T22:17:21Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-84a245cb9b144811b04124edb1d7139f2023-11-23T19:20:28ZengMDPI AGVaccines2076-393X2022-05-0110686210.3390/vaccines10060862Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot StudyAlessandra Panico0Giambattista Lobreglio1Francesco Bagordo2Antonella Zizza3Antonella De Donno4Chiara Rosato5Roberta Lazzari6Michele Chicone7Floriano Indino8Virginia Recchia9Pietro Alifano10Tiziana Grassi11Department of Biological and Environmental Science and Technology, University of Salento, 73100 Lecce, ItalyClinical Pathology and Microbiology Unit, Vito Fazzi General Hospital, 73100 Lecce, ItalyDepartment of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, 70121 Bari, ItalyInstitute of Clinical Physiology, National Research Council, 73100 Lecce, ItalyDepartment of Biological and Environmental Science and Technology, University of Salento, 73100 Lecce, ItalyClinical Pathology and Microbiology Unit, Vito Fazzi General Hospital, 73100 Lecce, ItalyClinical Pathology and Microbiology Unit, Vito Fazzi General Hospital, 73100 Lecce, ItalyClinical Pathology and Microbiology Unit, Vito Fazzi General Hospital, 73100 Lecce, ItalyClinical Pathology and Microbiology Unit, Vito Fazzi General Hospital, 73100 Lecce, ItalyInstitute of Clinical Physiology, National Research Council, 73100 Lecce, ItalyDepartment of Biological and Environmental Science and Technology, University of Salento, 73100 Lecce, ItalyDepartment of Biological and Environmental Science and Technology, University of Salento, 73100 Lecce, ItalyThe SARS-CoV-2 pandemic led to the development of various vaccines. The BNT162b2 mRNA vaccine was the first approved due to its efficacy in eliciting a humoral immunity response after the second dose. However, a decrease in the antibody concentration was observed over time. Therefore, the administration of a third dose was scheduled, primarily for frail people and workers of essential public activities. The aim of this study was to assess the level of antibodies against the spike (S) RBD of SARS-CoV-2 in healthcare workers before and after the third dose of BNT162b2 vaccine, according to sex, age, and the time interval between vaccine doses and tests. All 37 (12 males, 25 females, 19 < 50 years old, 18 ≥ 50 years old) healthcare workers recruited showed a consistent antibody titer increase after the third dose. Data analysis showed that the antibody concentration before the third dose significantly decreased as the time interval up to the test increased, and a significantly higher level was shown in young than older people. Cluster analysis revealed that young females had a higher antibody level than older females before the third dose (<i>p</i> < 0.05). This study indicated the benefit of the third dose of BNT162b2 vaccine and its effect on leveling up the humoral immune response.https://www.mdpi.com/2076-393X/10/6/862SARS-CoV-2BNT162b2 mRNA vaccinehealthcare workersbooster dose |
| spellingShingle | Alessandra Panico Giambattista Lobreglio Francesco Bagordo Antonella Zizza Antonella De Donno Chiara Rosato Roberta Lazzari Michele Chicone Floriano Indino Virginia Recchia Pietro Alifano Tiziana Grassi Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study Vaccines SARS-CoV-2 BNT162b2 mRNA vaccine healthcare workers booster dose |
| title | Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study |
| title_full | Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study |
| title_fullStr | Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study |
| title_full_unstemmed | Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study |
| title_short | Antibody Response in Healthcare Workers before and after the Third Dose of Anti-SARS-CoV-2 Vaccine: A Pilot Study |
| title_sort | antibody response in healthcare workers before and after the third dose of anti sars cov 2 vaccine a pilot study |
| topic | SARS-CoV-2 BNT162b2 mRNA vaccine healthcare workers booster dose |
| url | https://www.mdpi.com/2076-393X/10/6/862 |
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